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Imaging the interaction of roots and phosphate fertiliser granules using 4D X-ray tomography

Imaging the interaction of roots and phosphate fertiliser granules using 4D X-ray tomography
Imaging the interaction of roots and phosphate fertiliser granules using 4D X-ray tomography
Plant root system architecture adapts to the prevailing soil environment and the distribution of nutrients. Many species respond to localised regions of high nutrient supply, found in the vicinity of fertiliser granules, by elevating branching density in these areas. However, observation of these adaptations are frequently limited to plants cultured in idealised materials (e.g. hydrogels) which have a structure-less, homogenous matrix, or rhizotrons, which are spatially limited and provide only 2D data that are not fully quantitative.

Methods

In this study, in vivo, time resolved, non-destructive, micro-focus X-ray CT imaging (?CT) in 3D was used to visualise, quantify and assess root/fertiliser interactions of wheat plants in an agricultural soil during the entire plant life cycle. Two contrasting fertilisers [Triple superphosphate (TSP) and struvite (Crystal Green®)] were applied according to 3 different treatments, each providing an equivalent of 80 kg P2O5 ha-1 (struvite only, TSP only and a 50:50 mixture) to each plant. ?CT scans (60 ?m spatial resolution) of the plant roots were obtained over 14 weeks.

Results

This is the first time that in situ root/soil/fertiliser interactions have been visualised in 3D from plant germination through to maturity. Results show that lateral roots tend to pass within a few millimetres of the phosphorus (P) source. At this length scale, roots are able to access the P diffusing from the granule.

Conclusions

Quantitative analysis of root/fertiliser interactions has shown that rooting density correlates with granule volume-loss for a slow release, struvite fertiliser.
125-134
Ahmed, Sharif
ddc6bab1-9d76-4391-b7ea-ae68d6f3924d
Naugler Klassen, Trudy
f3e9db6a-f834-460c-97fa-49013cea54ea
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
Daly, Michael
e7b47d2a-a10e-4672-ae9f-2f2b3444dc86
Jones, David L.
3a1eb348-2f7c-44a6-ab80-b30b847ff933
Mavrogordato, Mark N.
f3e0879b-118a-463a-a130-1c890e9ab547
Sinclair, Ian
6005f6c1-f478-434e-a52d-d310c18ade0d
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Ahmed, Sharif
ddc6bab1-9d76-4391-b7ea-ae68d6f3924d
Naugler Klassen, Trudy
f3e9db6a-f834-460c-97fa-49013cea54ea
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
Daly, Michael
e7b47d2a-a10e-4672-ae9f-2f2b3444dc86
Jones, David L.
3a1eb348-2f7c-44a6-ab80-b30b847ff933
Mavrogordato, Mark N.
f3e0879b-118a-463a-a130-1c890e9ab547
Sinclair, Ian
6005f6c1-f478-434e-a52d-d310c18ade0d
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Ahmed, Sharif, Naugler Klassen, Trudy, Keyes, Samuel, Daly, Michael, Jones, David L., Mavrogordato, Mark N., Sinclair, Ian and Roose, Tiina (2016) Imaging the interaction of roots and phosphate fertiliser granules using 4D X-ray tomography. Plant and Soil, 401 (1), 125-134. (doi:10.1007/s11104-015-2425-5).

Record type: Article

Abstract

Plant root system architecture adapts to the prevailing soil environment and the distribution of nutrients. Many species respond to localised regions of high nutrient supply, found in the vicinity of fertiliser granules, by elevating branching density in these areas. However, observation of these adaptations are frequently limited to plants cultured in idealised materials (e.g. hydrogels) which have a structure-less, homogenous matrix, or rhizotrons, which are spatially limited and provide only 2D data that are not fully quantitative.

Methods

In this study, in vivo, time resolved, non-destructive, micro-focus X-ray CT imaging (?CT) in 3D was used to visualise, quantify and assess root/fertiliser interactions of wheat plants in an agricultural soil during the entire plant life cycle. Two contrasting fertilisers [Triple superphosphate (TSP) and struvite (Crystal Green®)] were applied according to 3 different treatments, each providing an equivalent of 80 kg P2O5 ha-1 (struvite only, TSP only and a 50:50 mixture) to each plant. ?CT scans (60 ?m spatial resolution) of the plant roots were obtained over 14 weeks.

Results

This is the first time that in situ root/soil/fertiliser interactions have been visualised in 3D from plant germination through to maturity. Results show that lateral roots tend to pass within a few millimetres of the phosphorus (P) source. At this length scale, roots are able to access the P diffusing from the granule.

Conclusions

Quantitative analysis of root/fertiliser interactions has shown that rooting density correlates with granule volume-loss for a slow release, struvite fertiliser.

Text
Imaging the interaction of roots and phosphate fertiliser granules using 4D X-ray tomography.pdf - Accepted Manuscript
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More information

Accepted/In Press date: 19 February 2015
e-pub ahead of print date: 13 March 2015
Published date: April 2016
Organisations: Bioengineering Group

Identifiers

Local EPrints ID: 374594
URI: https://eprints.soton.ac.uk/id/eprint/374594
PURE UUID: 1f292293-570c-46de-86c4-b1636809cc4a
ORCID for Samuel Keyes: ORCID iD orcid.org/0000-0002-4129-2228
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 25 Feb 2015 14:59
Last modified: 06 Jun 2018 12:36

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