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The predominant species of nonstructural protein 4B in hepatitis C virus-replicating cells is not palmitoylated

The predominant species of nonstructural protein 4B in hepatitis C virus-replicating cells is not palmitoylated
The predominant species of nonstructural protein 4B in hepatitis C virus-replicating cells is not palmitoylated
Hepatitis C virus (HCV) represents a significant global health burden. Viral replication is thought to occur in close association with remodelled host cell membranes with non-structural protein 4B (NS4B) being a key player in this process. NS4B is a poorly characterized integral membrane protein, which has been reported to be palmitoylated at its carboxy-terminal end. In order to extend this observation and to establish a functional role for NS4B palmitolylation, we sought to determine the status of this post-translational modification when the protein was expressed as part of a functional viral replicase. We performed direct metabolic labelling and polyethylene glycol-maleimide palmitoylation reporter assays on NS4B expressed in cells containing subgenomic replicons and infectious viral RNA. In a vaccinia virus-based expression system NS4B palmitoylation was detected in a genotype-dependent manner. However, in spite of the high sensitivity of the methods used, no NS4B palmitoylation was found in physiologically more relevant systems. Thus, NS4B palmitoylation is most likely dispensable for HCV RNA replication
0022-1317
1-15
Paul, David
3e159fbc-1caf-482f-a05f-7666f2c70443
Bartenschlager, Ralf
dadd023f-a584-48eb-bde0-d7213bc59ae9
McCormick, Christopher J.
0fce14bf-2f67-4d08-991f-114dd1e7f0bd
Paul, David
3e159fbc-1caf-482f-a05f-7666f2c70443
Bartenschlager, Ralf
dadd023f-a584-48eb-bde0-d7213bc59ae9
McCormick, Christopher J.
0fce14bf-2f67-4d08-991f-114dd1e7f0bd

Paul, David, Bartenschlager, Ralf and McCormick, Christopher J. (2015) The predominant species of nonstructural protein 4B in hepatitis C virus-replicating cells is not palmitoylated. Journal of General Virology, 1-15. (doi:10.1099/vir.0.000111). (PMID:25740959)

Record type: Article

Abstract

Hepatitis C virus (HCV) represents a significant global health burden. Viral replication is thought to occur in close association with remodelled host cell membranes with non-structural protein 4B (NS4B) being a key player in this process. NS4B is a poorly characterized integral membrane protein, which has been reported to be palmitoylated at its carboxy-terminal end. In order to extend this observation and to establish a functional role for NS4B palmitolylation, we sought to determine the status of this post-translational modification when the protein was expressed as part of a functional viral replicase. We performed direct metabolic labelling and polyethylene glycol-maleimide palmitoylation reporter assays on NS4B expressed in cells containing subgenomic replicons and infectious viral RNA. In a vaccinia virus-based expression system NS4B palmitoylation was detected in a genotype-dependent manner. However, in spite of the high sensitivity of the methods used, no NS4B palmitoylation was found in physiologically more relevant systems. Thus, NS4B palmitoylation is most likely dispensable for HCV RNA replication

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JGV NS4B palmitoylation paper.pdf - Accepted Manuscript
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Accepted/In Press date: 1 March 2015
e-pub ahead of print date: 4 March 2015
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 375093
URI: https://eprints.soton.ac.uk/id/eprint/375093
ISSN: 0022-1317
PURE UUID: 7f4fad37-3bc5-42bf-89e7-2a0ffb57f130

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Date deposited: 12 Mar 2015 09:52
Last modified: 17 Jul 2017 21:20

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Author: David Paul
Author: Ralf Bartenschlager

University divisions

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