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A low-noise transimpedance amplifier for BLM-based ion channel recording

A low-noise transimpedance amplifier for BLM-based ion channel recording
A low-noise transimpedance amplifier for BLM-based ion channel recording
High-throughput screening (HTS) using ion channel recording is a powerful drug discovery technique in pharmacology. Ion channel recording with planar bilayer lipid membranes (BLM) is scalable and has very high sensitivity. A HTS system based on BLM ion channel recording faces three main challenges: (i) design of scalable microfluidic devices; (ii) design of compact ultra-low-noise transimpedance amplifiers able to detect currents in the pA range with bandwidth >10 kHz; (iii) design of compact, robust and scalable systems that integrate these two elements. This paper presents a low-noise transimpedance amplifier with integrated A/D conversion realized in CMOS 0.35 µm technology. The CMOS amplifier acquires currents in the range ±200 pA and ±20 nA, with 100 kHz bandwidth while dissipating 41 mW. An integrated digital offset compensation loop balances any voltage offsets from Ag/AgCl electrodes. The measured open-input input-referred noise current is as low as 4 fA/Root Hz at ±200 pA range. The current amplifier is embedded in an integrated platform, together with a microfluidic device, for current recording from ion channels. Gramicidin-A, alpha-haemolysin and KcsA potassium channels have been used to prove both the platform and the current-to-digital converter.
transimpedance amplifier, current sensing circuit, low-noise amplifier, low-noise current sensor, noise, ion channel recording, bilayer lipid membranes, electrophysiology
1424-8220
1-20
Crescentini, Marco
568e8046-74bd-449f-8794-889643147059
Bennati, Marco
dd4ae84d-21e3-414b-a8c1-c4abbbf12c3e
Saha, Shimul C.
e2c8a09a-21af-4a31-bd25-26945094e03a
Ivica, Josip
aacf91f9-3876-4a95-8914-98e94cc524d7
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Tartagni, Marco
665c139f-e76b-49e2-8cf7-199478891464
Crescentini, Marco
568e8046-74bd-449f-8794-889643147059
Bennati, Marco
dd4ae84d-21e3-414b-a8c1-c4abbbf12c3e
Saha, Shimul C.
e2c8a09a-21af-4a31-bd25-26945094e03a
Ivica, Josip
aacf91f9-3876-4a95-8914-98e94cc524d7
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Tartagni, Marco
665c139f-e76b-49e2-8cf7-199478891464

Crescentini, Marco, Bennati, Marco, Saha, Shimul C., Ivica, Josip, de Planque, Maurits R.R., Morgan, Hywel and Tartagni, Marco (2016) A low-noise transimpedance amplifier for BLM-based ion channel recording. Sensors, 16 (5, 709), 1-20. (doi:10.3390/s16050709).

Record type: Article

Abstract

High-throughput screening (HTS) using ion channel recording is a powerful drug discovery technique in pharmacology. Ion channel recording with planar bilayer lipid membranes (BLM) is scalable and has very high sensitivity. A HTS system based on BLM ion channel recording faces three main challenges: (i) design of scalable microfluidic devices; (ii) design of compact ultra-low-noise transimpedance amplifiers able to detect currents in the pA range with bandwidth >10 kHz; (iii) design of compact, robust and scalable systems that integrate these two elements. This paper presents a low-noise transimpedance amplifier with integrated A/D conversion realized in CMOS 0.35 µm technology. The CMOS amplifier acquires currents in the range ±200 pA and ±20 nA, with 100 kHz bandwidth while dissipating 41 mW. An integrated digital offset compensation loop balances any voltage offsets from Ag/AgCl electrodes. The measured open-input input-referred noise current is as low as 4 fA/Root Hz at ±200 pA range. The current amplifier is embedded in an integrated platform, together with a microfluidic device, for current recording from ion channels. Gramicidin-A, alpha-haemolysin and KcsA potassium channels have been used to prove both the platform and the current-to-digital converter.

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More information

Submitted date: 11 December 2015
Accepted/In Press date: 5 May 2016
e-pub ahead of print date: 19 May 2016
Published date: 19 May 2016
Keywords: transimpedance amplifier, current sensing circuit, low-noise amplifier, low-noise current sensor, noise, ion channel recording, bilayer lipid membranes, electrophysiology
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 375224
URI: https://eprints.soton.ac.uk/id/eprint/375224
ISSN: 1424-8220
PURE UUID: 55342bbb-1f76-4f3b-a6cd-2cdee8dc98cd
ORCID for Maurits R.R. de Planque: ORCID iD orcid.org/0000-0002-8787-0513
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 16 Mar 2015 15:20
Last modified: 20 Jul 2019 00:59

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