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Transcriptome sequencing and marker development for four underutilized legumes

Transcriptome sequencing and marker development for four underutilized legumes
Transcriptome sequencing and marker development for four underutilized legumes
PREMISE OF THE STUDY:

Combating threats to food and nutrition security in the context of climate change and global population increase is one of the highest priorities of major international organizations. Hundreds of species are grown on a small scale in some of the most drought/flood-prone regions of the world and as such may harbor some of the most environmentally tolerant crops (and alleles).

METHODS AND RESULTS:

In this study, transcriptomes were sequenced, assembled, and annotated for four underutilized legume crops. Microsatellite markers were identified in each species, as well as a conserved orthologous set of markers for cross-family phylogenetics and comparative mapping, which were ground-truthed on a panel of diverse legume germplasm.

CONCLUSIONS:

An understanding of these underutilized legumes will inform crop selection and breeding by allowing the investigation of genetic variation and the genetic basis of adaptive traits to be established.
beans, drought, legumes, microsatellites, simple sequence repeats (SSRs), transcriptome
2168-0450
1400111-[6pp]
Chapman, Mark A.
8bac4a92-bfa7-4c3c-af29-9af852ef6383
Chapman, Mark A.
8bac4a92-bfa7-4c3c-af29-9af852ef6383

Chapman, Mark A. (2015) Transcriptome sequencing and marker development for four underutilized legumes. Applications in Plant Sciences, 3 (2), 1400111-[6pp]. (doi:10.3732/apps.1400111). (PMID:25699221)

Record type: Article

Abstract

PREMISE OF THE STUDY:

Combating threats to food and nutrition security in the context of climate change and global population increase is one of the highest priorities of major international organizations. Hundreds of species are grown on a small scale in some of the most drought/flood-prone regions of the world and as such may harbor some of the most environmentally tolerant crops (and alleles).

METHODS AND RESULTS:

In this study, transcriptomes were sequenced, assembled, and annotated for four underutilized legume crops. Microsatellite markers were identified in each species, as well as a conserved orthologous set of markers for cross-family phylogenetics and comparative mapping, which were ground-truthed on a panel of diverse legume germplasm.

CONCLUSIONS:

An understanding of these underutilized legumes will inform crop selection and breeding by allowing the investigation of genetic variation and the genetic basis of adaptive traits to be established.

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More information

Published date: 10 February 2015
Keywords: beans, drought, legumes, microsatellites, simple sequence repeats (SSRs), transcriptome
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 375378
URI: https://eprints.soton.ac.uk/id/eprint/375378
ISSN: 2168-0450
PURE UUID: f055b4bd-762c-4f9c-a9cd-335b74b37e50
ORCID for Mark A. Chapman: ORCID iD orcid.org/0000-0002-7151-723X

Catalogue record

Date deposited: 23 Mar 2015 11:57
Last modified: 20 Jul 2019 00:39

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