Droplet interfaced parallel and quantitative microfluidic-based separations
Droplet interfaced parallel and quantitative microfluidic-based separations
High-throughput, quantitative, and rapid microfluidic-based separations has been a long-sought goal for applications in proteomics, genomics, biomarker discovery, and clinical diagnostics. Using droplet-interfaced microchip electrophoresis (MCE) techniques, we have developed a novel parallel MCE platform, based on the concept of combining the Slipchip principle with a newly developed “Gelchip”. The platform consists of two plastic plates, with droplet wells on one plate and separation channels with preloaded/cured gel in the other. A single relative movement of one plate enables generation and then loading of multiple sample droplets in parallel into the separation channels, allowing electrophoretic separation of biomolecules in the droplets in parallel and with high-throughput. As proof of concept, we demonstrated the separation of 30 sub-nL sample droplets containing fluorescent dyes or DNA fragments.
3895-3901
Hassan, Sammer-ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
7 April 2015
Hassan, Sammer-ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
Hassan, Sammer-ul, Morgan, Hywel, Zhang, Xunli and Niu, Xize
(2015)
Droplet interfaced parallel and quantitative microfluidic-based separations.
Analytical Chemistry, 87 (7), .
(doi:10.1021/ac504695w).
Abstract
High-throughput, quantitative, and rapid microfluidic-based separations has been a long-sought goal for applications in proteomics, genomics, biomarker discovery, and clinical diagnostics. Using droplet-interfaced microchip electrophoresis (MCE) techniques, we have developed a novel parallel MCE platform, based on the concept of combining the Slipchip principle with a newly developed “Gelchip”. The platform consists of two plastic plates, with droplet wells on one plate and separation channels with preloaded/cured gel in the other. A single relative movement of one plate enables generation and then loading of multiple sample droplets in parallel into the separation channels, allowing electrophoretic separation of biomolecules in the droplets in parallel and with high-throughput. As proof of concept, we demonstrated the separation of 30 sub-nL sample droplets containing fluorescent dyes or DNA fragments.
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Accepted/In Press date: 16 March 2015
e-pub ahead of print date: 25 March 2015
Published date: 7 April 2015
Organisations:
Mechatronics
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Local EPrints ID: 375496
URI: http://eprints.soton.ac.uk/id/eprint/375496
ISSN: 0003-2700
PURE UUID: 2f3356db-693d-4575-a90f-e1089a5aaec6
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Date deposited: 27 Mar 2015 11:48
Last modified: 15 Mar 2024 03:29
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Author:
Hywel Morgan
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