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Iron deficiency induces a chlorophyll d-binding Pcb antenna system around Photosystem I in Acaryochloris marina.

Iron deficiency induces a chlorophyll d-binding Pcb antenna system around Photosystem I in Acaryochloris marina.
Iron deficiency induces a chlorophyll d-binding Pcb antenna system around Photosystem I in Acaryochloris marina.
The prochlorophyte-like cyanobacterium Acaryochloris marina contains two pcb genes, pcbA and pcbC, which encode chlorophyll (Chl) d-binding antenna proteins PcbA and PcbC, respectively. Using real-time reverse transcriptase polymerase chain reaction (RT-PCR), it is shown that when Acaryochloris cells are grown in an iron-deficient medium, the transcription of the pcbC gene is up-regulated compared to that of pcbA. Biochemical and immunological analyses indicated that under the same iron-deficient conditions, the level of Photosystem I (PSI) decreased compared with that of Photosystem II (PSII). Electron microscopy revealed that concomitant with these changes was the formation of Pcb-PSI supercomplexes which, in their largest form, were composed of 18 Pcb subunits forming a ring around the trimeric PSI reaction centre core. Mass spectrometry indicated that the PcbC protein is the main constituent of this outer PSI antenna system. It is therefore concluded that in Acaryochloris, the PcbC protein forms an antenna for PSI when iron levels become limiting and in this way compensates for the drop in the level of PSI relative to PSII which occurs under these conditions.
Acaryochloris, iron stress, photosynthesis, PCB protein, photosystem, gene transcript level
0005-2728
367-374
Chen, M.
bd112357-061f-4e59-9db8-648cd8fdde32
Bibby, T.S.
e04ea079-dd90-4ead-9840-00882de27ebd
Nield, J.
db2193b3-41b9-4db7-841f-81511e56effb
Larkum, A.
b6d82511-51c8-411b-aa77-ef17575aa3af
Barber, J.
a130af2c-9cc2-42f5-8a2b-0febcbdc6e2c
Chen, M.
bd112357-061f-4e59-9db8-648cd8fdde32
Bibby, T.S.
e04ea079-dd90-4ead-9840-00882de27ebd
Nield, J.
db2193b3-41b9-4db7-841f-81511e56effb
Larkum, A.
b6d82511-51c8-411b-aa77-ef17575aa3af
Barber, J.
a130af2c-9cc2-42f5-8a2b-0febcbdc6e2c

Chen, M., Bibby, T.S., Nield, J., Larkum, A. and Barber, J. (2005) Iron deficiency induces a chlorophyll d-binding Pcb antenna system around Photosystem I in Acaryochloris marina. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1708 (3), 367-374. (doi:10.1016/j.bbabio.2005.05.007).

Record type: Article

Abstract

The prochlorophyte-like cyanobacterium Acaryochloris marina contains two pcb genes, pcbA and pcbC, which encode chlorophyll (Chl) d-binding antenna proteins PcbA and PcbC, respectively. Using real-time reverse transcriptase polymerase chain reaction (RT-PCR), it is shown that when Acaryochloris cells are grown in an iron-deficient medium, the transcription of the pcbC gene is up-regulated compared to that of pcbA. Biochemical and immunological analyses indicated that under the same iron-deficient conditions, the level of Photosystem I (PSI) decreased compared with that of Photosystem II (PSII). Electron microscopy revealed that concomitant with these changes was the formation of Pcb-PSI supercomplexes which, in their largest form, were composed of 18 Pcb subunits forming a ring around the trimeric PSI reaction centre core. Mass spectrometry indicated that the PcbC protein is the main constituent of this outer PSI antenna system. It is therefore concluded that in Acaryochloris, the PcbC protein forms an antenna for PSI when iron levels become limiting and in this way compensates for the drop in the level of PSI relative to PSII which occurs under these conditions.

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More information

Published date: July 2005
Keywords: Acaryochloris, iron stress, photosynthesis, PCB protein, photosystem, gene transcript level

Identifiers

Local EPrints ID: 37557
URI: https://eprints.soton.ac.uk/id/eprint/37557
ISSN: 0005-2728
PURE UUID: 30d35ded-996b-4131-abd9-29bcfc08d146

Catalogue record

Date deposited: 23 May 2006
Last modified: 17 Jul 2017 15:42

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