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Iron deficiency induces a chlorophyll d-binding Pcb antenna system around Photosystem I in Acaryochloris marina.

Chen, M., Bibby, T.S., Nield, J., Larkum, A. and Barber, J. (2005) Iron deficiency induces a chlorophyll d-binding Pcb antenna system around Photosystem I in Acaryochloris marina. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1708, (3), pp. 367-374. (doi:10.1016/j.bbabio.2005.05.007).

Record type: Article

Abstract

The prochlorophyte-like cyanobacterium Acaryochloris marina contains two pcb genes, pcbA and pcbC, which encode chlorophyll (Chl) d-binding antenna proteins PcbA and PcbC, respectively. Using real-time reverse transcriptase polymerase chain reaction (RT-PCR), it is shown that when Acaryochloris cells are grown in an iron-deficient medium, the transcription of the pcbC gene is up-regulated compared to that of pcbA. Biochemical and immunological analyses indicated that under the same iron-deficient conditions, the level of Photosystem I (PSI) decreased compared with that of Photosystem II (PSII). Electron microscopy revealed that concomitant with these changes was the formation of Pcb-PSI supercomplexes which, in their largest form, were composed of 18 Pcb subunits forming a ring around the trimeric PSI reaction centre core. Mass spectrometry indicated that the PcbC protein is the main constituent of this outer PSI antenna system. It is therefore concluded that in Acaryochloris, the PcbC protein forms an antenna for PSI when iron levels become limiting and in this way compensates for the drop in the level of PSI relative to PSII which occurs under these conditions.

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More information

Published date: July 2005
Keywords: Acaryochloris, iron stress, photosynthesis, PCB protein, photosystem, gene transcript level

Identifiers

Local EPrints ID: 37557
URI: http://eprints.soton.ac.uk/id/eprint/37557
ISSN: 0005-2728
PURE UUID: 30d35ded-996b-4131-abd9-29bcfc08d146

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Date deposited: 23 May 2006
Last modified: 17 Jul 2017 15:42

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Contributors

Author: M. Chen
Author: T.S. Bibby
Author: J. Nield
Author: A. Larkum
Author: J. Barber

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