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The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja

The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja
The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja
Extant deep-sea invertebrate fauna represent both ancient and recent invasions from shallow-water habitats. Hydrostatic pressure may present a significant physiological challenge to organisms seeking to colonise deeper waters or migrate ontogenetically. Pressure may be a key factor contributing to bottlenecks in the radiation of taxa and potentially drive speciation. Here, we assess shifts in the tolerance of hydrostatic pressure through early ontogeny of the northern stone crab Lithodes maja, which occupies a depth range of 4-790 m in the North Atlantic. The zoea I, megalopa, and crab I stages were exposed to hydrostatic pressures up to 30.0 MPa (equivalent of 3000 m depth), and the relative fold change of genes putatively coding for the N-methyl-D-aspartate receptor-regulated protein 1 (narg gene), two heat shock protein 70kDa (HSP70) isoforms, and mitochondrial Citrate Synthase (CS gene) were measured. This study finds a significant increase in the relative expression of the CS and hsp70a genes with increased hydrostatic pressure in the zoea I stage, and an increase in the relative expression of all genes with increased hydrostatic pressure in the megalopa and crab I stages. Transcriptional responses are corroborated by patterns in respiratory rates in response to hydrostatic pressure in all stages. These results suggest a decrease in the acute high-pressure tolerance limit as ontogeny advances, as reflected by a shift in the hydrostatic pressure at which significant differences are observed.
deep sea, evolution, species radiation, Lithodidae, physiological bottleneck, hydrostatic pressure
0962-8452
50577
Munro, Catriona
c1f6933c-d4ad-47b5-8f7b-fccdd47d5b29
Morris, James P.
a25b0b80-d09e-4ec8-81bb-a9b659a539a6
Brown, Alastair
909f34db-bc9c-403f-ba8f-31aee1c00161
Hauton, Chris
7706f6ba-4497-42b2-8c6d-00df81676331
Thatje, Sven
f1011fe3-1048-40c0-97c1-e93b796e6533
Munro, Catriona
c1f6933c-d4ad-47b5-8f7b-fccdd47d5b29
Morris, James P.
a25b0b80-d09e-4ec8-81bb-a9b659a539a6
Brown, Alastair
909f34db-bc9c-403f-ba8f-31aee1c00161
Hauton, Chris
7706f6ba-4497-42b2-8c6d-00df81676331
Thatje, Sven
f1011fe3-1048-40c0-97c1-e93b796e6533

Munro, Catriona, Morris, James P., Brown, Alastair, Hauton, Chris and Thatje, Sven (2015) The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja. Proceedings of the Royal Society B: Biological Sciences, 282 (1809), 50577. (doi:10.1098/rspb.2015.0577).

Record type: Article

Abstract

Extant deep-sea invertebrate fauna represent both ancient and recent invasions from shallow-water habitats. Hydrostatic pressure may present a significant physiological challenge to organisms seeking to colonise deeper waters or migrate ontogenetically. Pressure may be a key factor contributing to bottlenecks in the radiation of taxa and potentially drive speciation. Here, we assess shifts in the tolerance of hydrostatic pressure through early ontogeny of the northern stone crab Lithodes maja, which occupies a depth range of 4-790 m in the North Atlantic. The zoea I, megalopa, and crab I stages were exposed to hydrostatic pressures up to 30.0 MPa (equivalent of 3000 m depth), and the relative fold change of genes putatively coding for the N-methyl-D-aspartate receptor-regulated protein 1 (narg gene), two heat shock protein 70kDa (HSP70) isoforms, and mitochondrial Citrate Synthase (CS gene) were measured. This study finds a significant increase in the relative expression of the CS and hsp70a genes with increased hydrostatic pressure in the zoea I stage, and an increase in the relative expression of all genes with increased hydrostatic pressure in the megalopa and crab I stages. Transcriptional responses are corroborated by patterns in respiratory rates in response to hydrostatic pressure in all stages. These results suggest a decrease in the acute high-pressure tolerance limit as ontogeny advances, as reflected by a shift in the hydrostatic pressure at which significant differences are observed.

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More information

Published date: 3 June 2015
Keywords: deep sea, evolution, species radiation, Lithodidae, physiological bottleneck, hydrostatic pressure
Organisations: Ocean and Earth Science, Southampton Marine & Maritime Institute

Identifiers

Local EPrints ID: 375806
URI: https://eprints.soton.ac.uk/id/eprint/375806
ISSN: 0962-8452
PURE UUID: b74db84a-dfc7-4abe-9e02-11169c1c68b2
ORCID for Chris Hauton: ORCID iD orcid.org/0000-0002-2313-4226

Catalogue record

Date deposited: 09 Apr 2015 10:49
Last modified: 06 Jun 2018 13:03

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