Jeppesen, C.A., Snape, M.D., Robinson, H.., Gossger, N., John, T.M., Voysey, M., Ladhani, S., Okike, I.O., Oeser, C., Kent, A., Oliver, J., Taylor, Peter K., Morales-Aza, B., Clarke, Stuart C., Casey, M., Martins, F., Kitchin, N.R., Anderson, A.S., Jones, H., Jansen, K.U., Eiden, J., Peneault, L., Heath, P.T., Finn, A., Faust, S.N. and Pollard, A.J. (2015) Meningococcal carriage in adolescents in the United Kingdom to inform timing of an adolescent vaccination strategy. Journal of Infection, 1-10. (doi:10.1016/j.jinf.2015.02.006). (PMID:25709085)
Abstract
Objectives
Recent development of serogroup B meningococcal (MenB) vaccines highlights the importance of pharyngeal carriage data, particularly in adolescents and young adults, to inform implementation strategies. We describe current UK carriage prevalence in this high risk population and compare methods of carriage detection.
Methods
In this multisite study, pharyngeal swabs were collected on 3–4 occasions over 6–12 months, from 1040 school and university students, aged 10–25 years. Meningococcal carriage was detected by standard culture combined with seroagglutination or PCR of cultured isolates, or by direct PCR from swab. The factor H binding protein (fHBP) variants present in meningococcal isolates were determined.
Results
Meningococcal serogroups B and Y were most common, with carriage up to 6.5% and 5.5% respectively, increasing throughout adolescence. Identification by seroagglutination was often unreliable, and the sensitivity of direct PCR detection was 66% compared to culture combined with PCR. Of MenB isolates, 89.1% had subfamily A variants of fHBP. The acquisition rate of MenB carriage was estimated at 2.8 per 1000 person-months.
Conclusions
If vaccination is to precede the adolescent rise in MenB carriage, these data suggest it should take place in early adolescence. Studies assessing vaccine impact should use molecular methods to detect carriage.
This record has no associated files available for download.
More information
Identifiers
Catalogue record
Export record
Altmetrics
Contributors
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.