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Size-fractionated diversity of eukaryotic microbial communities in the Eastern Tropical North Pacific oxygen minimum zone

Size-fractionated diversity of eukaryotic microbial communities in the Eastern Tropical North Pacific oxygen minimum zone
Size-fractionated diversity of eukaryotic microbial communities in the Eastern Tropical North Pacific oxygen minimum zone
Oxygen Minimum Zones (OMZs) caused by water column stratification appear to expand in parts of the world's ocean, with consequences for marine biogeochemical cycles. OMZ formation is often fueled by high surface primary production, and sinking organic particles can be hotspots of interactions and activity within microbial communities. This study investigated the diversity of OMZ protist communities in two biomass size fractions (>30 and 30–1.6 ?m filters) from the world's largest permanent OMZ in the Eastern Tropical North Pacific. Diversity was quantified via Illumina MiSeq sequencing of V4 region of 18S SSU rRNA genes in samples spanning oxygen gradients at two stations. Alveolata and Rhizaria dominated the two size fractions at both sites along the oxygen gradient. Community composition at finer taxonomic levels was partially shaped by oxygen concentration, as communities associated with vs. anoxic waters shared only ?32% of OTU (97% sequence identity) composition. Overall, only 9.7% of total OTUs were recovered at both stations and under all oxygen conditions sampled, implying structuring of the eukaryotic community in this area. Size-fractionated communities exhibited different taxonomical features (e.g. Syndiniales Group I in the 1.6–30?m fraction) that could be explained by the microniches created on the surface-originated sinking particles.
protist diversity, 18S SSU rRNA, particle-associated, water-column
fiv037
Duret, M.T.
e9f43140-067d-45d4-b7ea-68dd432798ea
Pachiadaki, M.G.
f59f9202-fb6b-460c-9fb4-e6761ee443ac
Stewart, F.J.
8fea21c9-2716-4484-b80f-bb00ce8d0e23
Sarode, N.
9567cb9f-39d8-4f05-9985-b40da292f6e0
Christaki, U.
c00e99d1-4c85-4f7e-9b81-78ede7bb10e5
Monchy, S.
f966787e-ea86-44be-8a87-eaf38a287197
Edgcomb, V.P.
27dda2a9-203b-44db-b39d-d473e247aed7
Duret, M.T.
e9f43140-067d-45d4-b7ea-68dd432798ea
Pachiadaki, M.G.
f59f9202-fb6b-460c-9fb4-e6761ee443ac
Stewart, F.J.
8fea21c9-2716-4484-b80f-bb00ce8d0e23
Sarode, N.
9567cb9f-39d8-4f05-9985-b40da292f6e0
Christaki, U.
c00e99d1-4c85-4f7e-9b81-78ede7bb10e5
Monchy, S.
f966787e-ea86-44be-8a87-eaf38a287197
Edgcomb, V.P.
27dda2a9-203b-44db-b39d-d473e247aed7

Duret, M.T., Pachiadaki, M.G., Stewart, F.J., Sarode, N., Christaki, U., Monchy, S. and Edgcomb, V.P. (2015) Size-fractionated diversity of eukaryotic microbial communities in the Eastern Tropical North Pacific oxygen minimum zone. FEMS Microbiology Ecology, 91 (5), fiv037. (doi:10.1093/femsec/fiv037).

Record type: Article

Abstract

Oxygen Minimum Zones (OMZs) caused by water column stratification appear to expand in parts of the world's ocean, with consequences for marine biogeochemical cycles. OMZ formation is often fueled by high surface primary production, and sinking organic particles can be hotspots of interactions and activity within microbial communities. This study investigated the diversity of OMZ protist communities in two biomass size fractions (>30 and 30–1.6 ?m filters) from the world's largest permanent OMZ in the Eastern Tropical North Pacific. Diversity was quantified via Illumina MiSeq sequencing of V4 region of 18S SSU rRNA genes in samples spanning oxygen gradients at two stations. Alveolata and Rhizaria dominated the two size fractions at both sites along the oxygen gradient. Community composition at finer taxonomic levels was partially shaped by oxygen concentration, as communities associated with vs. anoxic waters shared only ?32% of OTU (97% sequence identity) composition. Overall, only 9.7% of total OTUs were recovered at both stations and under all oxygen conditions sampled, implying structuring of the eukaryotic community in this area. Size-fractionated communities exhibited different taxonomical features (e.g. Syndiniales Group I in the 1.6–30?m fraction) that could be explained by the microniches created on the surface-originated sinking particles.

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More information

e-pub ahead of print date: 2 April 2015
Keywords: protist diversity, 18S SSU rRNA, particle-associated, water-column
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 376032
URI: http://eprints.soton.ac.uk/id/eprint/376032
PURE UUID: adb53eed-0d46-4149-b16e-030950d05923
ORCID for M.T. Duret: ORCID iD orcid.org/0000-0002-0922-4372

Catalogue record

Date deposited: 13 Apr 2015 12:40
Last modified: 24 Jun 2022 21:59

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Contributors

Author: M.T. Duret ORCID iD
Author: M.G. Pachiadaki
Author: F.J. Stewart
Author: N. Sarode
Author: U. Christaki
Author: S. Monchy
Author: V.P. Edgcomb

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