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Multicolor RGB marking allows morphometric and functional analysis of hippocampal granule neurons at the single-cell level

Multicolor RGB marking allows morphometric and functional analysis of hippocampal granule neurons at the single-cell level
Multicolor RGB marking allows morphometric and functional analysis of hippocampal granule neurons at the single-cell level
Identifying and tracking cells in the brain is a challenging task, requiring advanced molecular biology techniques and precise anatomical information. We have applied the principle of RGB marking to the long-term marking and tracking of progenitor and mature cells in the adult brain. Briefly, multicolor RGB marking is based on the simultaneous, lentiviral vector-mediated expression of three genes encoding fluorescent proteins in the three basic colors (red, green, and blue). Here, we show the application of RGB marking to the stable multicolor marking of adult granule cells in the hippocampus. This technique provides each individual cell with a characteristic hue, which facilitates their anatomical identification and spatial tracking. Multicolor RBG marking of mature neurons can provide an effective approach to dissect the function of individual cells, as it allows combination with functional techniques, facilitating the understanding of complex neuronal populations such as that of the dentate gyrus.
1043-0342
Gomez-Nicola, Diego
0680aa66-9dee-47cf-a8d3-e39c988f85b5
Riecken, K.
386307f1-46f2-4774-8d0a-40df7e0fe7d5
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Fehse, B.
42f61f07-238b-4441-89be-aef92d350fef
Gomez-Nicola, Diego
0680aa66-9dee-47cf-a8d3-e39c988f85b5
Riecken, K.
386307f1-46f2-4774-8d0a-40df7e0fe7d5
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Fehse, B.
42f61f07-238b-4441-89be-aef92d350fef

Gomez-Nicola, Diego, Riecken, K., Perry, V. Hugh and Fehse, B. (2015) Multicolor RGB marking allows morphometric and functional analysis of hippocampal granule neurons at the single-cell level. Human Gene Therapy. (doi:10.1089/hum.2015.010). (PMID:25894663)

Record type: Article

Abstract

Identifying and tracking cells in the brain is a challenging task, requiring advanced molecular biology techniques and precise anatomical information. We have applied the principle of RGB marking to the long-term marking and tracking of progenitor and mature cells in the adult brain. Briefly, multicolor RGB marking is based on the simultaneous, lentiviral vector-mediated expression of three genes encoding fluorescent proteins in the three basic colors (red, green, and blue). Here, we show the application of RGB marking to the stable multicolor marking of adult granule cells in the hippocampus. This technique provides each individual cell with a characteristic hue, which facilitates their anatomical identification and spatial tracking. Multicolor RBG marking of mature neurons can provide an effective approach to dissect the function of individual cells, as it allows combination with functional techniques, facilitating the understanding of complex neuronal populations such as that of the dentate gyrus.

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More information

Accepted/In Press date: 2 March 2015
Published date: 20 April 2015
Organisations: Biomedicine

Identifiers

Local EPrints ID: 376446
URI: https://eprints.soton.ac.uk/id/eprint/376446
ISSN: 1043-0342
PURE UUID: 603dc8f9-42c1-41bf-9b12-5be367369995
ORCID for Diego Gomez-Nicola: ORCID iD orcid.org/0000-0002-5316-2682

Catalogue record

Date deposited: 28 Apr 2015 13:21
Last modified: 06 Jun 2018 12:33

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