Placental amino acid transport may be regulated by maternal vitamin D and vitamin D-binding protein: results from the Southampton Women’s Survey
Placental amino acid transport may be regulated by maternal vitamin D and vitamin D-binding protein: results from the Southampton Women’s Survey
Both maternal 25-hydroxyvitamin D [25(OH)D] concentrations during pregnancy and placental amino acid transporter gene expression have been associated with development of the offspring in terms of body composition and bone structure. Several amino acid transporter genes have vitamin D response elements in their promoters suggesting the possible linkage of these two mechanisms. We aimed to establish whether maternal 25(OH)D and vitamin D-binding protein (DBP) levels relate to expression of placental amino acid transporters. RNA was extracted from 102 placental samples collected in the Southampton Women’s Survey (SWS) and gene expression was analysed using quantitative real-time PCR. Gene expression data were normalised to the geometric mean of three housekeeping genes and related to maternal factors and childhood body composition. Maternal serum 25(OH)D and DBP levels were measured by radioimmunoassay. Maternal 25(OH)D and DBP levels were positively associated with placental expression of specific genes involved in amino acid transport. Maternal 25(OH)D and DBP concentrations were correlated with the expression of specific placental amino acid transporters and thus may be involved in the regulation of amino acid transfer to the fetus. The positive correlation of DBP levels and placental transporter expression suggests that delivery of vitamin D to the placenta may be important. This exploratory study identifies placental amino acid transporters which may be altered in response to modifiable maternal factors and provides a basis for further studies.
1903-1910
Cleal, J.K.
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Day, P.E.L.
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Simner, C.L.
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Barton, Sheila J.
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Mahon, Pamela A.
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Inskip, H.M.
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Godfrey, K.M.
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Hanson, M.A.
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Cooper, C.
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Lewis, Rohan M.
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Harvey, Nicholas C.
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28 June 2015
Cleal, J.K.
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Day, P.E.L.
c5db77dc-3829-43ed-ba46-ece124d323d3
Simner, C.L.
82600725-9c0f-4030-b978-1699abb7a1f5
Barton, Sheila J.
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Mahon, Pamela A.
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Inskip, H.M.
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Godfrey, K.M.
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Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Cooper, C.
e05f5612-b493-4273-9b71-9e0ce32bdad6
Lewis, Rohan M.
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Harvey, Nicholas C.
ce487fb4-d360-4aac-9d17-9466d6cba145
Cleal, J.K., Day, P.E.L. and Simner, C.L. et al.
(2015)
Placental amino acid transport may be regulated by maternal vitamin D and vitamin D-binding protein: results from the Southampton Women’s Survey.
British Journal of Nutrition, 113 (12), .
(doi:10.1017/S0007114515001178).
Abstract
Both maternal 25-hydroxyvitamin D [25(OH)D] concentrations during pregnancy and placental amino acid transporter gene expression have been associated with development of the offspring in terms of body composition and bone structure. Several amino acid transporter genes have vitamin D response elements in their promoters suggesting the possible linkage of these two mechanisms. We aimed to establish whether maternal 25(OH)D and vitamin D-binding protein (DBP) levels relate to expression of placental amino acid transporters. RNA was extracted from 102 placental samples collected in the Southampton Women’s Survey (SWS) and gene expression was analysed using quantitative real-time PCR. Gene expression data were normalised to the geometric mean of three housekeeping genes and related to maternal factors and childhood body composition. Maternal serum 25(OH)D and DBP levels were measured by radioimmunoassay. Maternal 25(OH)D and DBP levels were positively associated with placental expression of specific genes involved in amino acid transport. Maternal 25(OH)D and DBP concentrations were correlated with the expression of specific placental amino acid transporters and thus may be involved in the regulation of amino acid transfer to the fetus. The positive correlation of DBP levels and placental transporter expression suggests that delivery of vitamin D to the placenta may be important. This exploratory study identifies placental amino acid transporters which may be altered in response to modifiable maternal factors and provides a basis for further studies.
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Accepted/In Press date: 16 March 2015
e-pub ahead of print date: 5 May 2015
Published date: 28 June 2015
Organisations:
Human Development & Health
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Local EPrints ID: 376709
URI: http://eprints.soton.ac.uk/id/eprint/376709
ISSN: 0007-1145
PURE UUID: c3200e41-a29c-4c87-af93-80dc9d575b81
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Date deposited: 06 May 2015 15:42
Last modified: 18 Mar 2024 02:58
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Author:
P.E.L. Day
Author:
C.L. Simner
Author:
Pamela A. Mahon
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