The University of Southampton
University of Southampton Institutional Repository

Bacteriophage and phenotypic variation in Pseudomonas aeruginosa biofilm development

Record type: Article

A current question in biofilm research is whether biofilm-specific genetic processes can lead to differentiation in physiology and function among biofilm cells. In Pseudomonas aeruginosa, phenotypic variants which exhibit a small-colony phenotype on agar media and a markedly accelerated pattern of biofilm development compared to that of the parental strain are often isolated from biofilms. We grew P. aeruginosa biofilms in glass flow cell reactors and observed that the emergence of small-colony variants (SCVs) in the effluent runoff from the biofilms correlated with the emergence of plaque-forming Pf1-like filamentous phage (designated Pf4) from the biofilm. Because several recent studies have shown that bacteriophage genes are among the most highly upregulated groups of genes during biofilm development, we investigated whether Pf4 plays a role in SCV formation during P. aeruginosa biofilm development. We carried out immunoelectron microscopy using anti- Pf4 antibodies and observed that SCV cells, but not parental-type cells, exhibited high densities of Pf4 filaments on the cell surface and that these filaments were often tightly interwoven into complex latticeworks surrounding the cells. Moreover, infection of P. aeruginosa planktonic cultures with Pf4 caused the emergence of SCVs within the culture. These SCVs exhibited enhanced attachment, accelerated biofilm development, and large regions of dead and lysed cells inside microcolonies in a manner identical to that of SCVs obtained from biofilms. We concluded that Pf4 can mediate phenotypic variation in P. aeruginosa biofilms. We also performed partial sequencing and analysis of the Pf4 replicative form and identified a number of open reading frames not previously recognized in the genome of P. aeruginosa, including a putative postsegregational killing operon.

PDF Webb_et_al_04,_J_Bacteriol.pdf - Version of Record
Restricted to Registered users only
Download (355kB)

Citation

Webb, Jeremy S., Lau, Mathew and Kjelleberg, Staffan (2004) Bacteriophage and phenotypic variation in Pseudomonas aeruginosa biofilm development Journal of Bacteriology, 186, (23), pp. 8066-8073. (doi:10.1128/JB.186.23.8066-8073.2004).

More information

Published date: December 2004

Identifiers

Local EPrints ID: 37683
URI: http://eprints.soton.ac.uk/id/eprint/37683
ISSN: 0021-9193
PURE UUID: fe785318-90f1-46a4-860d-51ce52eaae05
ORCID for Jeremy S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

Catalogue record

Date deposited: 25 May 2006
Last modified: 17 Jul 2017 15:41

Export record

Altmetrics

Contributors

Author: Jeremy S. Webb ORCID iD
Author: Mathew Lau
Author: Staffan Kjelleberg

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×