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Rhamnolipid production from waste cooking oil using Pseudomonas SWP-4

Rhamnolipid production from waste cooking oil using Pseudomonas SWP-4
Rhamnolipid production from waste cooking oil using Pseudomonas SWP-4
The present work aims to produce rhamnolipid from waste cooking oil (WCO) using a newly isolated bacterium named Pseudomonas SWP-4. SWP-4 was a high-yield strain that could accumulate rhamnolipid steadily even in decline phase and gave a maximum rhamnolipid yield of 13.93 g/L and WCO utilization percent around 88%. The critical micelle concentration of the produced rhamnolipid was only 27 mg/L and its emulsification index against n-hexadecane reached around 59%. Moreover, it reduced the surface tension of water from 71.8 mN/m to 24.1 mN/m and the interfacial tension against n-hexadecane from 29.4 mN/m to 0.9 mN/m. Results of biosurfactant stability show the rhamnolipid was effective when the salinity was lower than 8% and pH value ranged from 4 to 10, and it was quite thermostable based on thermal gravity analysis. Furthermore, it maintained high surface activity even after incubation under extreme conditions i.e. pH of 4.0, salinity of 8% and temperature of 80°C for half a month. Based on free fatty acids metabolism analysis, Pseudomonas SWP-4 consumed palmitic acid, oleic acid and linoleic acid chiefly. All these characteristics demonstrate bioconversion and biodegradation of WCO by Pseudomonas SWP-4 is a promising and commercial way of rhamnolipid production and waste treatment.
rhamnolipid, waste cooking oil, production kinetics, bioconversion, biodegradation, waste treatment
1369-703X
44-54
Lan, Guihong
0ae877a8-10a5-4c24-add4-c4c9c8f0ec43
Fan, Qiang
e3c612b3-bd58-42c9-ba1d-415d133aa566
Liu, Yongqiang
75adc6f8-aa83-484e-9e87-6c8442e344fa
Chen, Chao
22c894ec-9b6f-4c4d-91cc-ee710112e582
Li, Guixiang
8da4a8ea-1d6d-45d8-9d55-f5535cdeadad
Liu, Yu
7aa0a5b8-dca4-4f81-b6c9-7332b1beb25c
Yin, Xiaobo
122fe1d9-e108-4a42-821f-29f0a5906acb
Lan, Guihong
0ae877a8-10a5-4c24-add4-c4c9c8f0ec43
Fan, Qiang
e3c612b3-bd58-42c9-ba1d-415d133aa566
Liu, Yongqiang
75adc6f8-aa83-484e-9e87-6c8442e344fa
Chen, Chao
22c894ec-9b6f-4c4d-91cc-ee710112e582
Li, Guixiang
8da4a8ea-1d6d-45d8-9d55-f5535cdeadad
Liu, Yu
7aa0a5b8-dca4-4f81-b6c9-7332b1beb25c
Yin, Xiaobo
122fe1d9-e108-4a42-821f-29f0a5906acb

Lan, Guihong, Fan, Qiang, Liu, Yongqiang, Chen, Chao, Li, Guixiang, Liu, Yu and Yin, Xiaobo (2015) Rhamnolipid production from waste cooking oil using Pseudomonas SWP-4. Biochemical Engineering Journal, 101, 44-54. (doi:10.1016/j.bej.2015.05.001).

Record type: Article

Abstract

The present work aims to produce rhamnolipid from waste cooking oil (WCO) using a newly isolated bacterium named Pseudomonas SWP-4. SWP-4 was a high-yield strain that could accumulate rhamnolipid steadily even in decline phase and gave a maximum rhamnolipid yield of 13.93 g/L and WCO utilization percent around 88%. The critical micelle concentration of the produced rhamnolipid was only 27 mg/L and its emulsification index against n-hexadecane reached around 59%. Moreover, it reduced the surface tension of water from 71.8 mN/m to 24.1 mN/m and the interfacial tension against n-hexadecane from 29.4 mN/m to 0.9 mN/m. Results of biosurfactant stability show the rhamnolipid was effective when the salinity was lower than 8% and pH value ranged from 4 to 10, and it was quite thermostable based on thermal gravity analysis. Furthermore, it maintained high surface activity even after incubation under extreme conditions i.e. pH of 4.0, salinity of 8% and temperature of 80°C for half a month. Based on free fatty acids metabolism analysis, Pseudomonas SWP-4 consumed palmitic acid, oleic acid and linoleic acid chiefly. All these characteristics demonstrate bioconversion and biodegradation of WCO by Pseudomonas SWP-4 is a promising and commercial way of rhamnolipid production and waste treatment.

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More information

Accepted/In Press date: 2 May 2015
e-pub ahead of print date: 5 May 2015
Published date: 15 September 2015
Keywords: rhamnolipid, waste cooking oil, production kinetics, bioconversion, biodegradation, waste treatment
Organisations: Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 376918
URI: http://eprints.soton.ac.uk/id/eprint/376918
ISSN: 1369-703X
PURE UUID: 3921f92a-fbda-4ad3-b926-12d62a2c7905

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Date deposited: 13 May 2015 08:49
Last modified: 10 Dec 2019 06:38

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Contributors

Author: Guihong Lan
Author: Qiang Fan
Author: Yongqiang Liu
Author: Chao Chen
Author: Guixiang Li
Author: Yu Liu
Author: Xiaobo Yin

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