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Polymersomes prepared from thermoresponsive fluorescent protein-polymer bioconjugates: capture of and report on drug and protein payloads

Polymersomes prepared from thermoresponsive fluorescent protein-polymer bioconjugates: capture of and report on drug and protein payloads
Polymersomes prepared from thermoresponsive fluorescent protein-polymer bioconjugates: capture of and report on drug and protein payloads
Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report where the components are in the polymersomes. To address these twin challenges, we synthesized the protein–polymer bioconjugate PNIPAM-b-amilFP497 composed of thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) and a green-fluorescent protein variant (amilFP497). Above 37?°C, this bioconjugate forms polymersomes that can (co-)encapsulate the fluorescent drug doxorubicin and the fluorescent light-harvesting protein phycoerythrin?545 (PE545). Using fluorescence lifetime imaging microscopy and Förster resonance energy transfer (FLIM-FRET), we can distinguish the co-encapsulated PE545 protein inside the polymersome membrane while doxorubicin is found both in the polymersome core and membrane.
Einkapselung, FRET, Kompartimentierung, Polymersome, Proteinmodifikationen
0044-8249
5407-5412
Wong, Chin Ken
c58e89c8-076a-40da-a3d1-03381f7cbc26
Laos, Alistair J.
b62b0541-3b55-45f3-8eb5-4b2f2dafaa69
Soeriyadi, Alexander H.
93452551-bdd2-44a7-940b-afeedafe4b57
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Curmi, Paul M.G.
7b01816c-9216-4f4f-80c6-638713ab720a
Gooding, J. Justin
e349b903-88e1-4ef6-900f-2b4ec6e834f1
Marquis, Christopher P.
c60a5b09-5b79-412d-afd8-b93998c445c2
Stenzel, Martina H.
b457f4ef-4817-45d1-ac65-397e7c3f9598
Thordarson, Pall
9d3b961d-c277-46ca-9eef-1bc77133fb94
Wong, Chin Ken
c58e89c8-076a-40da-a3d1-03381f7cbc26
Laos, Alistair J.
b62b0541-3b55-45f3-8eb5-4b2f2dafaa69
Soeriyadi, Alexander H.
93452551-bdd2-44a7-940b-afeedafe4b57
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Curmi, Paul M.G.
7b01816c-9216-4f4f-80c6-638713ab720a
Gooding, J. Justin
e349b903-88e1-4ef6-900f-2b4ec6e834f1
Marquis, Christopher P.
c60a5b09-5b79-412d-afd8-b93998c445c2
Stenzel, Martina H.
b457f4ef-4817-45d1-ac65-397e7c3f9598
Thordarson, Pall
9d3b961d-c277-46ca-9eef-1bc77133fb94

Wong, Chin Ken, Laos, Alistair J., Soeriyadi, Alexander H., Wiedenmann, Jörg, Curmi, Paul M.G., Gooding, J. Justin, Marquis, Christopher P., Stenzel, Martina H. and Thordarson, Pall (2015) Polymersomes prepared from thermoresponsive fluorescent protein-polymer bioconjugates: capture of and report on drug and protein payloads. Angewandte Chemie, 127 (18), 5407-5412. (doi:10.1002/ange.201412406).

Record type: Article

Abstract

Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report where the components are in the polymersomes. To address these twin challenges, we synthesized the protein–polymer bioconjugate PNIPAM-b-amilFP497 composed of thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) and a green-fluorescent protein variant (amilFP497). Above 37?°C, this bioconjugate forms polymersomes that can (co-)encapsulate the fluorescent drug doxorubicin and the fluorescent light-harvesting protein phycoerythrin?545 (PE545). Using fluorescence lifetime imaging microscopy and Förster resonance energy transfer (FLIM-FRET), we can distinguish the co-encapsulated PE545 protein inside the polymersome membrane while doxorubicin is found both in the polymersome core and membrane.

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More information

e-pub ahead of print date: 3 March 2015
Published date: 27 April 2015
Keywords: Einkapselung, FRET, Kompartimentierung, Polymersome, Proteinmodifikationen
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 376943
URI: http://eprints.soton.ac.uk/id/eprint/376943
ISSN: 0044-8249
PURE UUID: f5e2e0f6-0b6d-41c0-bd40-44ef0ee9ac05
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

Catalogue record

Date deposited: 08 May 2015 14:37
Last modified: 28 Apr 2022 01:58

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Contributors

Author: Chin Ken Wong
Author: Alistair J. Laos
Author: Alexander H. Soeriyadi
Author: Paul M.G. Curmi
Author: J. Justin Gooding
Author: Christopher P. Marquis
Author: Martina H. Stenzel
Author: Pall Thordarson

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