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Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research

Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research
Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research
Lack of reproducibility is an ongoing problem in some areas of the biomedical sciences. Poor experimental design and a failure to engage with experienced statisticians at key stages in the design and analysis of experiments are two factors that contribute to this problem. The RIPOSTE (Reducing IrreProducibility in labOratory STudiEs) framework has been developed to support early and regular discussions between scientists and statisticians in order to improve the design, conduct and analysis of laboratory studies and, therefore, reduce irreproducibility. The framework is intended for using during the early stages of a research project, when specific questions or hypotheses are proposed. The essential points within the framework are explained and illustrated using three examples (a medical equipment test, a macrophage study and a gene expression study). Sound study design minimises the possibility of bias being introduced into experiments and leads to higher quality research with more reproducible results.
2050-084X
1-49
Masca, Nicholas G.D.
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Hensor, Elizabeth M.A.
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Cornelius, Victoria R.
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Buffa, Francesca M.
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Marriott, Helen M.
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Eales, James M.
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Messenger, Michael P.
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Anderson, Amy E.
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Boot, Chris
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Bunce, Catey
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Goldin, Robert D.
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Harris, Jessica
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Hinchliffe, Rod F.
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Junaid, Hiba
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Kingston, Shaun
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Martin-Ruiz, Carmen
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Nelson, Christopher P.
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Peacock, Janet
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Seed, Paul T.
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Shinkins, Bethany
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Staples, Karl J.
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Toombs, Jamie
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Wright, Adam K.A.
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Teare, M. Dawn
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Masca, Nicholas G.D.
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Hensor, Elizabeth M.A.
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Cornelius, Victoria R.
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Buffa, Francesca M.
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Marriott, Helen M.
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Eales, James M.
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Messenger, Michael P.
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Anderson, Amy E.
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Boot, Chris
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Bunce, Catey
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Goldin, Robert D.
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Harris, Jessica
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Hinchliffe, Rod F.
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Junaid, Hiba
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Kingston, Shaun
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Martin-Ruiz, Carmen
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Nelson, Christopher P.
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Peacock, Janet
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Seed, Paul T.
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Shinkins, Bethany
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Staples, Karl J.
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Toombs, Jamie
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Wright, Adam K.A.
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Teare, M. Dawn
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Masca, Nicholas G.D., Hensor, Elizabeth M.A., Cornelius, Victoria R., Buffa, Francesca M., Marriott, Helen M., Eales, James M., Messenger, Michael P., Anderson, Amy E., Boot, Chris, Bunce, Catey, Goldin, Robert D., Harris, Jessica, Hinchliffe, Rod F., Junaid, Hiba, Kingston, Shaun, Martin-Ruiz, Carmen, Nelson, Christopher P., Peacock, Janet, Seed, Paul T., Shinkins, Bethany, Staples, Karl J., Toombs, Jamie, Wright, Adam K.A. and Teare, M. Dawn (2015) Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research. eLife, 4, 1-49. (doi:10.7554/eLife.05519). (PMID:25951517)

Record type: Article

Abstract

Lack of reproducibility is an ongoing problem in some areas of the biomedical sciences. Poor experimental design and a failure to engage with experienced statisticians at key stages in the design and analysis of experiments are two factors that contribute to this problem. The RIPOSTE (Reducing IrreProducibility in labOratory STudiEs) framework has been developed to support early and regular discussions between scientists and statisticians in order to improve the design, conduct and analysis of laboratory studies and, therefore, reduce irreproducibility. The framework is intended for using during the early stages of a research project, when specific questions or hypotheses are proposed. The essential points within the framework are explained and illustrated using three examples (a medical equipment test, a macrophage study and a gene expression study). Sound study design minimises the possibility of bias being introduced into experiments and leads to higher quality research with more reproducible results.

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eLife.05519.full.pdf - Accepted Manuscript
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Published date: 7 May 2015
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 376964
URI: https://eprints.soton.ac.uk/id/eprint/376964
ISSN: 2050-084X
PURE UUID: e7b90686-9c23-4482-bad3-92f2eee39122
ORCID for Karl J. Staples: ORCID iD orcid.org/0000-0003-3844-6457

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Date deposited: 11 May 2015 14:33
Last modified: 06 Jun 2018 12:39

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Contributors

Author: Nicholas G.D. Masca
Author: Elizabeth M.A. Hensor
Author: Victoria R. Cornelius
Author: Francesca M. Buffa
Author: Helen M. Marriott
Author: James M. Eales
Author: Michael P. Messenger
Author: Amy E. Anderson
Author: Chris Boot
Author: Catey Bunce
Author: Robert D. Goldin
Author: Jessica Harris
Author: Rod F. Hinchliffe
Author: Hiba Junaid
Author: Shaun Kingston
Author: Carmen Martin-Ruiz
Author: Christopher P. Nelson
Author: Janet Peacock
Author: Paul T. Seed
Author: Bethany Shinkins
Author: Karl J. Staples ORCID iD
Author: Jamie Toombs
Author: Adam K.A. Wright
Author: M. Dawn Teare

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