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Molecular monitoring of chronic myeloid leukemia: principles and interlaboratory standardization

Molecular monitoring of chronic myeloid leukemia: principles and interlaboratory standardization
Molecular monitoring of chronic myeloid leukemia: principles and interlaboratory standardization
Serial quantification of BCR-ABL1 messenger RNA (mRNA) is an important therapeutic indicator for patients with chronic myeloid leukemia, but historically, there has been substantial variation in results reported by different laboratories. To help improve the comparability of results, an international scale (IS) for BCR-ABL1 was proposed which is being implemented by testing laboratories worldwide. This is being achieved most commonly by the derivation of laboratory-specific conversion factors, but increasingly by the use of kits or reagents that are calibrated to the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL1 mRNA. Recent attention has focused on the need to define and validate levels of deeper molecular response (MR) within the context of the IS. While there has been substantial progress in the alignment of results, BCR-ABL1 measurement is technically challenging and standardization is an ongoing process.











chronic myeloid leukemia, bcr-abl1 mrna, bcr-abl1 measurement
219-225
Cross, Nicholas C. P.
f87650da-b908-4a34-b31b-d62c5f186fe4
Hochhaus, Andreas
b37b9b7d-85ff-455e-994d-fcc2adf94088
Müller, Martin C.
ea37982c-00bb-44b5-bc73-f4b5bd0825e9
Cross, Nicholas C. P.
f87650da-b908-4a34-b31b-d62c5f186fe4
Hochhaus, Andreas
b37b9b7d-85ff-455e-994d-fcc2adf94088
Müller, Martin C.
ea37982c-00bb-44b5-bc73-f4b5bd0825e9

Cross, Nicholas C. P., Hochhaus, Andreas and Müller, Martin C. (2015) Molecular monitoring of chronic myeloid leukemia: principles and interlaboratory standardization. Annals of Hematology, 94 (S2), 219-225. (doi:10.1007/s00277-015-2315-1).

Record type: Article

Abstract

Serial quantification of BCR-ABL1 messenger RNA (mRNA) is an important therapeutic indicator for patients with chronic myeloid leukemia, but historically, there has been substantial variation in results reported by different laboratories. To help improve the comparability of results, an international scale (IS) for BCR-ABL1 was proposed which is being implemented by testing laboratories worldwide. This is being achieved most commonly by the derivation of laboratory-specific conversion factors, but increasingly by the use of kits or reagents that are calibrated to the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL1 mRNA. Recent attention has focused on the need to define and validate levels of deeper molecular response (MR) within the context of the IS. While there has been substantial progress in the alignment of results, BCR-ABL1 measurement is technically challenging and standardization is an ongoing process.











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More information

Published date: April 2015
Keywords: chronic myeloid leukemia, bcr-abl1 mrna, bcr-abl1 measurement
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 377000
URI: http://eprints.soton.ac.uk/id/eprint/377000
PURE UUID: 8e71e388-cb17-444d-b200-657372b7829d
ORCID for Nicholas C. P. Cross: ORCID iD orcid.org/0000-0001-5481-2555

Catalogue record

Date deposited: 18 May 2015 12:24
Last modified: 15 Mar 2024 03:11

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Contributors

Author: Andreas Hochhaus
Author: Martin C. Müller

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