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Structure–function relationships in fluorescent marker proteins of the green fluorescent protein family

Structure–function relationships in fluorescent marker proteins of the green fluorescent protein family
Structure–function relationships in fluorescent marker proteins of the green fluorescent protein family
GFP-like proteins, originally cloned from marine animals, are genetically encoded fluorescence markers that have become indispensable tools for the life sciences. The search for GFP-like proteins with novel and improved properties is ongoing, driven by the persistent need for advanced and specialized fluorescence labels for cellular imaging. The 3D structures of these proteins are overall similar. However, considerable variations have been found in the covalent structures and the stereochemistry of the chromophore, which govern essential optical properties such as the absorption/emission wavelengths. A detailed understanding of the structure and dynamics of GFP-like proteins greatly aids in the rational engineering of advanced fluorescence marker proteins. In this chapter, we summarize the present knowledge of the structural diversity of GFP-like proteins and discuss how structure and dynamics govern their optical properties.
Chromophore, Fluorescent protein, GFP
978-3-642-23371-5
241-263
Springer
Nienhaus, G. Ulrich
64eb2ac6-4fa9-416c-a066-f096d79307cb
Nienhaus, Karin
b05bb626-b88d-48d8-b0ed-cc1624de52ca
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Jung, G.
Nienhaus, G. Ulrich
64eb2ac6-4fa9-416c-a066-f096d79307cb
Nienhaus, Karin
b05bb626-b88d-48d8-b0ed-cc1624de52ca
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Jung, G.

Nienhaus, G. Ulrich, Nienhaus, Karin and Wiedenmann, Jörg (2012) Structure–function relationships in fluorescent marker proteins of the green fluorescent protein family. In, Jung, G. (ed.) Fluorescent Proteins I: From understanding to design. (Springer Series on Fluorescence, 11) Dordrecht, NL. Springer, pp. 241-263. (doi:10.1007/4243_2011_15).

Record type: Book Section

Abstract

GFP-like proteins, originally cloned from marine animals, are genetically encoded fluorescence markers that have become indispensable tools for the life sciences. The search for GFP-like proteins with novel and improved properties is ongoing, driven by the persistent need for advanced and specialized fluorescence labels for cellular imaging. The 3D structures of these proteins are overall similar. However, considerable variations have been found in the covalent structures and the stereochemistry of the chromophore, which govern essential optical properties such as the absorption/emission wavelengths. A detailed understanding of the structure and dynamics of GFP-like proteins greatly aids in the rational engineering of advanced fluorescence marker proteins. In this chapter, we summarize the present knowledge of the structural diversity of GFP-like proteins and discuss how structure and dynamics govern their optical properties.

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More information

Published date: 2012
Keywords: Chromophore, Fluorescent protein, GFP
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 377018
URI: http://eprints.soton.ac.uk/id/eprint/377018
ISBN: 978-3-642-23371-5
PURE UUID: 9d203c1b-659b-40a4-b781-136f7bbed436
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

Catalogue record

Date deposited: 13 May 2015 10:19
Last modified: 12 Dec 2021 03:37

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Contributors

Author: G. Ulrich Nienhaus
Author: Karin Nienhaus
Editor: G. Jung

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