The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity
The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity
Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.
sarcolipin, serca, thermogenesis, energy expenditure, obesity, high fat diet
26-31
Butler, John
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Smyth, Neil
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Broadbridge, Robert
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Council, Claire E.
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Lee, Anthony G.
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Stocker, Claire J.
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Hislop, David C.
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Arch, Jonathan R.S.
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Cawthorne, Michael A.
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East, J. Malcolm
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1 March 2015
Butler, John
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Smyth, Neil
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Broadbridge, Robert
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Council, Claire E.
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Lee, Anthony G.
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Stocker, Claire J.
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Hislop, David C.
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Arch, Jonathan R.S.
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Cawthorne, Michael A.
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East, J. Malcolm
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Butler, John, Smyth, Neil, Broadbridge, Robert, Council, Claire E., Lee, Anthony G., Stocker, Claire J., Hislop, David C., Arch, Jonathan R.S., Cawthorne, Michael A. and East, J. Malcolm
(2015)
The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity.
Archives of Biochemistry and Biophysics, 569, .
(doi:10.1016/j.abb.2015.01.027).
Abstract
Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.
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e-pub ahead of print date: 7 February 2015
Published date: 1 March 2015
Keywords:
sarcolipin, serca, thermogenesis, energy expenditure, obesity, high fat diet
Organisations:
Faculty of Natural and Environmental Sciences, Centre for Biological Sciences
Identifiers
Local EPrints ID: 377092
URI: http://eprints.soton.ac.uk/id/eprint/377092
ISSN: 0003-9861
PURE UUID: 00d8176d-e9c6-468e-b3c6-5cf8dcfdf8be
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Date deposited: 19 May 2015 12:41
Last modified: 20 Jun 2024 01:53
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Contributors
Author:
Robert Broadbridge
Author:
Claire E. Council
Author:
Anthony G. Lee
Author:
Claire J. Stocker
Author:
David C. Hislop
Author:
Jonathan R.S. Arch
Author:
Michael A. Cawthorne
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