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The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity

The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity
The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity
Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.
sarcolipin, serca, thermogenesis, energy expenditure, obesity, high fat diet
0003-9861
26-31
Butler, John
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Smyth, Neil
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Broadbridge, Robert
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Council, Claire E.
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Lee, Anthony G.
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Stocker, Claire J.
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Hislop, David C.
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Arch, Jonathan R.S.
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Cawthorne, Michael A.
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Malcolm East, J.
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Butler, John
5132f0e2-f28d-412a-be9a-e97963b4c0ee
Smyth, Neil
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Broadbridge, Robert
4f9e9a5c-45ed-4640-92d7-283933e42f60
Council, Claire E.
94b09834-9c03-4a4c-8c5a-01d9d9a0c5f0
Lee, Anthony G.
61ff8691-d4b6-4db4-8401-fc29998607b6
Stocker, Claire J.
852f32d4-5a51-40a7-8e2a-b314ddba1080
Hislop, David C.
c50d3518-a8ef-4cb9-909d-a24b4a23a48d
Arch, Jonathan R.S.
bf915ad2-21c7-4221-8888-ca66735244c3
Cawthorne, Michael A.
60647868-5616-4aba-977c-677697cd7bbe
Malcolm East, J.
9fe7f794-1d89-4935-9a99-b831d786056e

Butler, John, Smyth, Neil, Broadbridge, Robert, Council, Claire E., Lee, Anthony G., Stocker, Claire J., Hislop, David C., Arch, Jonathan R.S., Cawthorne, Michael A. and Malcolm East, J. (2015) The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity. Archives of Biochemistry and Biophysics, 569, 26-31. (doi:10.1016/j.abb.2015.01.027).

Record type: Article

Abstract

Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.

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e-pub ahead of print date: 7 February 2015
Published date: 1 March 2015
Keywords: sarcolipin, serca, thermogenesis, energy expenditure, obesity, high fat diet
Organisations: Faculty of Natural and Environmental Sciences, Centre for Biological Sciences

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Local EPrints ID: 377092
URI: https://eprints.soton.ac.uk/id/eprint/377092
ISSN: 0003-9861
PURE UUID: 00d8176d-e9c6-468e-b3c6-5cf8dcfdf8be

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Date deposited: 19 May 2015 12:41
Last modified: 19 Jul 2019 20:45

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