The University of Southampton
University of Southampton Institutional Repository

Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines

Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines
Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines
Development of synthetic surfaces that are highly reproducible and biocompatible for in vitro cell culture offers potential for development of improved models for studies of cellular physiology and pathology. They may also be useful in tissue engineering by removal of the need for biologically-derived components such as extracellular matrix proteins. We synthesised four types of 2-alkyl-2-oxazoline polymers ranging from the hydrophilic poly(2- methyl-2-oxazoline) to the hydrophobic poly(2-n-butyl-2-oxazoline). The polymers were terminated using amine-functionalised glass coverslips, enabling the synthetic procedure to be reproducible and scaleable. The polymer-coated glass slides were tested for biocompatibility using human epithelial (16HBE 14o-) and fibroblastic (MRC5) cell lines. Differences in adhesion and motility of the two cell types was observed, with the poly(2- isopropyl-2-oxazoline) polymer equally supporting the growth of both cell types, whereas poly(2-n-butyl-2-oxazoline) showed selectivity for fibroblast growth. In summary, 2-alkyl-2- oxazoline polymers may be a useful tool for building in vitro model cell culture models with preferential adhesion of specific cell types.
biocompatibility, cell proliferation, cell adhesion, co-culture, lung, synthetic polymer
0142-9612
26-32
Tait, Angela
91d3e403-54d5-4efd-94c5-8b476e6af283
Fisher, Adam L.
12c568b9-b402-4def-a0d0-b17688e4b1dc
Hartland, Tom
30ca163a-c3bb-40d0-a2bb-8200f69bccb1
Smart, David
3468eafa-5280-40a1-9fda-1bdc07dd914f
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Swindle, Emily J.
fe393c7a-a513-4de4-b02e-27369bd7e84f
Grossel, Martin
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Tait, Angela
91d3e403-54d5-4efd-94c5-8b476e6af283
Fisher, Adam L.
12c568b9-b402-4def-a0d0-b17688e4b1dc
Hartland, Tom
30ca163a-c3bb-40d0-a2bb-8200f69bccb1
Smart, David
3468eafa-5280-40a1-9fda-1bdc07dd914f
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Swindle, Emily J.
fe393c7a-a513-4de4-b02e-27369bd7e84f
Grossel, Martin
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38

Tait, Angela, Fisher, Adam L., Hartland, Tom, Smart, David, Glynne-Jones, Peter, Hill, Martyn, Swindle, Emily J., Grossel, Martin and Davies, Donna E. (2015) Biocompatibility of poly(2-alkyl-2-oxazoline) brush surfaces for adherent lung cell lines. Biomaterials, 61, 26-32. (doi:10.1016/j.biomaterials.2015.04.059). (PMID:25993014)

Record type: Article

Abstract

Development of synthetic surfaces that are highly reproducible and biocompatible for in vitro cell culture offers potential for development of improved models for studies of cellular physiology and pathology. They may also be useful in tissue engineering by removal of the need for biologically-derived components such as extracellular matrix proteins. We synthesised four types of 2-alkyl-2-oxazoline polymers ranging from the hydrophilic poly(2- methyl-2-oxazoline) to the hydrophobic poly(2-n-butyl-2-oxazoline). The polymers were terminated using amine-functionalised glass coverslips, enabling the synthetic procedure to be reproducible and scaleable. The polymer-coated glass slides were tested for biocompatibility using human epithelial (16HBE 14o-) and fibroblastic (MRC5) cell lines. Differences in adhesion and motility of the two cell types was observed, with the poly(2- isopropyl-2-oxazoline) polymer equally supporting the growth of both cell types, whereas poly(2-n-butyl-2-oxazoline) showed selectivity for fibroblast growth. In summary, 2-alkyl-2- oxazoline polymers may be a useful tool for building in vitro model cell culture models with preferential adhesion of specific cell types.

Text
__soton.ac.uk_ude_personalfiles_users_sb24_mydesktop_Tait et al_Biomaterials PostPrint.pdf - Other
Download (376kB)

More information

Accepted/In Press date: 30 April 2015
e-pub ahead of print date: 14 May 2015
Published date: August 2015
Keywords: biocompatibility, cell proliferation, cell adhesion, co-culture, lung, synthetic polymer
Organisations: Chemistry, NIHR Southampton Respiratory Biomedical Research Unit, Mechatronics

Identifiers

Local EPrints ID: 377324
URI: http://eprints.soton.ac.uk/id/eprint/377324
ISSN: 0142-9612
PURE UUID: f8d3c1ce-c442-491e-afb9-1dc4884e1008
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for Emily J. Swindle: ORCID iD orcid.org/0000-0003-3644-7747
ORCID for Martin Grossel: ORCID iD orcid.org/0000-0001-7469-6854
ORCID for Donna E. Davies: ORCID iD orcid.org/0000-0002-5117-2991

Catalogue record

Date deposited: 10 Jun 2015 11:29
Last modified: 18 Feb 2021 17:13

Export record

Altmetrics

Contributors

Author: Angela Tait
Author: Adam L. Fisher
Author: Tom Hartland
Author: David Smart
Author: Martyn Hill ORCID iD
Author: Martin Grossel ORCID iD
Author: Donna E. Davies ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×