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Production of siderophore type chelates by mixed bacterioplankton populations in nutrient enriched seawater incubations

Production of siderophore type chelates by mixed bacterioplankton populations in nutrient enriched seawater incubations
Production of siderophore type chelates by mixed bacterioplankton populations in nutrient enriched seawater incubations
Siderophore type chelates were isolated from nutrient enriched seawater collected from coastal and near shore environments and detected using a novel high performance liquid chromatography – electrospray ionisation – mass spectrometric technique. Seawater was enriched with added glucose, ammonia and phosphate, and incubated for four days. Seven different siderophore type compounds were detected in the extracted supernatants and tentatively identified based on mass numbers and spectra. The compounds comprised two groups, the ferrioxamines and the amphibactins. They were produced at typical coastal iron concentrations (total dissolved iron = 2.9 +/- 1.4 nM and 2.2 +/- 0.1 nM) both in the presence and absence of the iron chelating ligand ethylene diamine-N,N-diacetic acid.
iron, siderophores, chelates, ferrioxamines, amphibactins, HPLC-ESI-MS, coastal waters
0304-4203
75-83
Gledhill, M.
da795c1e-1489-4d40-9df1-fc6bde54382d
McCormack, P.
9ea72032-9c03-4b74-88db-42b845df636e
Ussher, S.
25e332ba-ce47-4590-9881-0e01aa6bdc92
Achterberg, E.P.
685ce961-8c45-4503-9f03-50f6561202b9
Mantoura, R.F.C.
fb0c3cf3-3a51-4557-a732-bd623cf703a0
Worsfold, P.
9bfded88-7405-4d69-95b3-ca9bd9513393
Gledhill, M.
da795c1e-1489-4d40-9df1-fc6bde54382d
McCormack, P.
9ea72032-9c03-4b74-88db-42b845df636e
Ussher, S.
25e332ba-ce47-4590-9881-0e01aa6bdc92
Achterberg, E.P.
685ce961-8c45-4503-9f03-50f6561202b9
Mantoura, R.F.C.
fb0c3cf3-3a51-4557-a732-bd623cf703a0
Worsfold, P.
9bfded88-7405-4d69-95b3-ca9bd9513393

Gledhill, M., McCormack, P., Ussher, S., Achterberg, E.P., Mantoura, R.F.C. and Worsfold, P. (2004) Production of siderophore type chelates by mixed bacterioplankton populations in nutrient enriched seawater incubations. Marine Chemistry, 88 (1-2), 75-83. (doi:10.1016/j.marchem.2004.03.003).

Record type: Article

Abstract

Siderophore type chelates were isolated from nutrient enriched seawater collected from coastal and near shore environments and detected using a novel high performance liquid chromatography – electrospray ionisation – mass spectrometric technique. Seawater was enriched with added glucose, ammonia and phosphate, and incubated for four days. Seven different siderophore type compounds were detected in the extracted supernatants and tentatively identified based on mass numbers and spectra. The compounds comprised two groups, the ferrioxamines and the amphibactins. They were produced at typical coastal iron concentrations (total dissolved iron = 2.9 +/- 1.4 nM and 2.2 +/- 0.1 nM) both in the presence and absence of the iron chelating ligand ethylene diamine-N,N-diacetic acid.

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Submitted date: October 2003
Published date: 2004
Keywords: iron, siderophores, chelates, ferrioxamines, amphibactins, HPLC-ESI-MS, coastal waters

Identifiers

Local EPrints ID: 37736
URI: http://eprints.soton.ac.uk/id/eprint/37736
ISSN: 0304-4203
PURE UUID: 09d6eacc-df61-46f9-bc97-939c8241d6d3

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Date deposited: 25 May 2006
Last modified: 15 Mar 2024 08:01

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Contributors

Author: M. Gledhill
Author: P. McCormack
Author: S. Ussher
Author: E.P. Achterberg
Author: R.F.C. Mantoura
Author: P. Worsfold

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