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Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide

Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide
Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide
In this work we demonstrate a DNA biosensor based on fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er nanoparticles and graphene oxide (GO). Monodisperse NaYF4:Yb,Er nanoparticles with a mean diameter of 29.1 ± 2.2 nm were synthesized and coated with a SiO2 shell of 11 nm, which allowed the attachment of single strands of DNA. When these DNA-functionalized NaYF4:Yb,Er@SiO2 nanoparticles were in the proximity of the GO surface, the ?–? stacking interaction between the nucleobases of the DNA and the sp2 carbons of the GO induced a FRET fluorescence quenching due to the overlap of the fluorescence emission of the NaYF4:Yb,Er@SiO2 and the absorption spectrum of GO. By contrast, in the presence of the complementary DNA strands, the hybridization leads to double-stranded DNA that does not interact with the GO surface, and thus the NaYF4:Yb,Er@SiO2 nanoparticles remain unquenched and fluorescent. The high sensitivity and specificity of this sensor introduces a new method for the detection of DNA with a detection limit of 5 pM.
1944-8244
1-8
Alonso-Cristobal, P.
8badd4be-b605-40c9-8610-cb04cd326177
Vilela, P.
5948c49e-b217-49e6-b689-4e41264b4449
El-Sagheer, A.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Lopez-Cabarcos, E.
b717ce52-ba79-4727-82d7-e3c52d7a9cd0
Brown, T.
65b220ab-5839-4e03-b923-97694339baaf
Muskens, O.L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Rubio-Retama, J.
10c1f28b-f66b-4d66-aec8-0879414031bc
Kanaras, A.G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Alonso-Cristobal, P.
8badd4be-b605-40c9-8610-cb04cd326177
Vilela, P.
5948c49e-b217-49e6-b689-4e41264b4449
El-Sagheer, A.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Lopez-Cabarcos, E.
b717ce52-ba79-4727-82d7-e3c52d7a9cd0
Brown, T.
65b220ab-5839-4e03-b923-97694339baaf
Muskens, O.L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Rubio-Retama, J.
10c1f28b-f66b-4d66-aec8-0879414031bc
Kanaras, A.G.
667ecfdc-7647-4bd8-be03-a47bf32504c7

Alonso-Cristobal, P., Vilela, P., El-Sagheer, A., Lopez-Cabarcos, E., Brown, T., Muskens, O.L., Rubio-Retama, J. and Kanaras, A.G. (2015) Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide. ACS Applied Materials & Interfaces, 1-8. (doi:10.1021/am507591u).

Record type: Article

Abstract

In this work we demonstrate a DNA biosensor based on fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er nanoparticles and graphene oxide (GO). Monodisperse NaYF4:Yb,Er nanoparticles with a mean diameter of 29.1 ± 2.2 nm were synthesized and coated with a SiO2 shell of 11 nm, which allowed the attachment of single strands of DNA. When these DNA-functionalized NaYF4:Yb,Er@SiO2 nanoparticles were in the proximity of the GO surface, the ?–? stacking interaction between the nucleobases of the DNA and the sp2 carbons of the GO induced a FRET fluorescence quenching due to the overlap of the fluorescence emission of the NaYF4:Yb,Er@SiO2 and the absorption spectrum of GO. By contrast, in the presence of the complementary DNA strands, the hybridization leads to double-stranded DNA that does not interact with the GO surface, and thus the NaYF4:Yb,Er@SiO2 nanoparticles remain unquenched and fluorescent. The high sensitivity and specificity of this sensor introduces a new method for the detection of DNA with a detection limit of 5 pM.

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Upconversion-DNA corrections R3N.docx - Other
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More information

Accepted/In Press date: 15 January 2015
Published date: 27 January 2015
Organisations: Quantum, Light & Matter Group

Identifiers

Local EPrints ID: 377404
URI: https://eprints.soton.ac.uk/id/eprint/377404
ISSN: 1944-8244
PURE UUID: c8b1a3cb-2764-4c0a-bdca-0521fcb687f1
ORCID for O.L. Muskens: ORCID iD orcid.org/0000-0003-0693-5504
ORCID for A.G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 11 Jun 2015 14:11
Last modified: 06 Jun 2018 12:37

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