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Separation and detection of siderophores produced by marine bacterioplankton using high-performance liquid chromatography with electrospray ionization mass spectrometry

Separation and detection of siderophores produced by marine bacterioplankton using high-performance liquid chromatography with electrospray ionization mass spectrometry
Separation and detection of siderophores produced by marine bacterioplankton using high-performance liquid chromatography with electrospray ionization mass spectrometry
High-performance liquid chromatography-electrospray ionization mass spectrometry was applied to the detection of the iron(III) complexes of the hydroxamate siderophores rhodotoluric acid, deferrioxamine B, and deferrichrome. Separation of the iron(III) complexes was obtained using a polystyrene-divinylbenzene stationary phase. The retention and responses of ferrioxamine and ferrichrome were optimal when a gradient elution program with methanol and 0.1% (v/v) formic acid as the mobile phases was used. These conditions were also suitable for the retention and separation of the uncomplexed ligands. Retention of iron(III) rhodotoluate was improved when formic acid was replaced by the ion-pairing reagent heptafluorobutyric acid (0.1%). Detection limits for the ferric complexes, defined as 3 SD of the lowest determined standard, were 26 nM for iron(III) rhodotoluate, 0.23 nM for ferrioxamine, and 0.40 nM for ferrichrome. A protocol for the solid-phase extraction of these hydroxamate siderophores from seawater was developed and applied to the extraction of siderophores from enriched incubated seawater samples.
equatorial pacific-ocean, phytoplankton bloom, iron fertilization' desferrioxamine, speciation, sediments, seawater, bacteria
0003-2700
2647-2652
McCormack, Paul
8193aa6e-165e-4c86-8b38-34ef7cddf83d
Worsfold, Paul J.
27675f89-7eee-45c5-821e-a381d8db9693
Gledhill, Martha
da795c1e-1489-4d40-9df1-fc6bde54382d
McCormack, Paul
8193aa6e-165e-4c86-8b38-34ef7cddf83d
Worsfold, Paul J.
27675f89-7eee-45c5-821e-a381d8db9693
Gledhill, Martha
da795c1e-1489-4d40-9df1-fc6bde54382d

McCormack, Paul, Worsfold, Paul J. and Gledhill, Martha (2003) Separation and detection of siderophores produced by marine bacterioplankton using high-performance liquid chromatography with electrospray ionization mass spectrometry. Analytical Chemistry, 75 (11), 2647-2652. (doi:10.1021/ac0340105).

Record type: Article

Abstract

High-performance liquid chromatography-electrospray ionization mass spectrometry was applied to the detection of the iron(III) complexes of the hydroxamate siderophores rhodotoluric acid, deferrioxamine B, and deferrichrome. Separation of the iron(III) complexes was obtained using a polystyrene-divinylbenzene stationary phase. The retention and responses of ferrioxamine and ferrichrome were optimal when a gradient elution program with methanol and 0.1% (v/v) formic acid as the mobile phases was used. These conditions were also suitable for the retention and separation of the uncomplexed ligands. Retention of iron(III) rhodotoluate was improved when formic acid was replaced by the ion-pairing reagent heptafluorobutyric acid (0.1%). Detection limits for the ferric complexes, defined as 3 SD of the lowest determined standard, were 26 nM for iron(III) rhodotoluate, 0.23 nM for ferrioxamine, and 0.40 nM for ferrichrome. A protocol for the solid-phase extraction of these hydroxamate siderophores from seawater was developed and applied to the extraction of siderophores from enriched incubated seawater samples.

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More information

Published date: 1 June 2003
Keywords: equatorial pacific-ocean, phytoplankton bloom, iron fertilization' desferrioxamine, speciation, sediments, seawater, bacteria

Identifiers

Local EPrints ID: 37741
URI: https://eprints.soton.ac.uk/id/eprint/37741
ISSN: 0003-2700
PURE UUID: 0852c896-0ab1-4a1d-898c-3161c1606339

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Date deposited: 25 May 2006
Last modified: 26 Feb 2019 17:31

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