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Biochemical and molecular changes associated with heteroxylan biosynthesis in Neolamarckia cadamba (Rubiaceae) during xylogenesis

Biochemical and molecular changes associated with heteroxylan biosynthesis in Neolamarckia cadamba (Rubiaceae) during xylogenesis
Biochemical and molecular changes associated with heteroxylan biosynthesis in Neolamarckia cadamba (Rubiaceae) during xylogenesis
Wood, derived from plant secondary growth, is a commercially important material. Both cellulose and lignin assembly have been well studied during wood formation (xylogenesis), but heteroxylan biosynthesis is less well defined. Elucidation of the heteroxylan biosynthetic pathway is crucial to understand the mechanism of wood formation. Here, we use Neolamarckia cadamba, a fast-growing tropical tree, as a sample to analyze heteroxylan formation at the biochemical and molecular levels during wood formation. Analysis of the non-cellulosic polysaccharides isolated from N. cadamba stems shows that heteroxylans dominate non-cellulosic polysaccharides and increase with xylogenesis. Microsomes isolated from stems of 1-year-old N. cadamba exhibited UDP-Xyl synthase and xylosyltransferase activities with the highest activity present in the middle and basal stem regions. To further understand the genetic basis of heteroxylan synthesis, RNA sequencing (RNA-seq) was used to generate transcriptomes of N. cadamba during xylogenesis. The RNA-seq results showed that genes related to heteroxylan synthesis had higher expression levels in the middle and basal part of the stem compared to the apical part. Our results describe the heteroxylan distribution and heteroxylan synthesis trait in N. cadamba and give a new example for understanding the mechanism of heteroxylan synthesis in tropical tree species in future
1664-462X
602
Zhao, Xianhai
06147a38-fe45-4a9e-869b-48b4341a756d
Ouyang, Kunxi
f67b2a6b-6a72-435a-bae3-03f685c645e7
Gan, Siming
63d8cc98-4b46-4cb6-a290-97b44c1e46b5
Zeng, Wei
41945831-4e12-44fe-aa5f-8d817dff8553
Song, Lili
8a7e8f36-310f-49e9-a7b7-5834766f6ecb
Zhao, Shuai
465082f4-30c7-4398-8cab-b64f17f7fa96
Li, Juncheng
9390a88c-6ebb-42d0-89be-32cdefb8e54d
Doblin, Monika S.
f08a429c-41d3-4e02-949c-ab44929b481d
Bacic, Antony
f0631c18-03f4-48be-83ce-0f840da2b67a
Chen, Xiao-Yang
21a6d271-cbee-49ce-a761-7771545b891b
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Deng, Xiaomei
28afc492-f857-4f70-aac7-d32855aced77
Wu, Ai-Min
03895642-6808-4ba4-8def-3fbc9935aee0
Zhao, Xianhai
06147a38-fe45-4a9e-869b-48b4341a756d
Ouyang, Kunxi
f67b2a6b-6a72-435a-bae3-03f685c645e7
Gan, Siming
63d8cc98-4b46-4cb6-a290-97b44c1e46b5
Zeng, Wei
41945831-4e12-44fe-aa5f-8d817dff8553
Song, Lili
8a7e8f36-310f-49e9-a7b7-5834766f6ecb
Zhao, Shuai
465082f4-30c7-4398-8cab-b64f17f7fa96
Li, Juncheng
9390a88c-6ebb-42d0-89be-32cdefb8e54d
Doblin, Monika S.
f08a429c-41d3-4e02-949c-ab44929b481d
Bacic, Antony
f0631c18-03f4-48be-83ce-0f840da2b67a
Chen, Xiao-Yang
21a6d271-cbee-49ce-a761-7771545b891b
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Deng, Xiaomei
28afc492-f857-4f70-aac7-d32855aced77
Wu, Ai-Min
03895642-6808-4ba4-8def-3fbc9935aee0

Zhao, Xianhai, Ouyang, Kunxi, Gan, Siming, Zeng, Wei, Song, Lili, Zhao, Shuai, Li, Juncheng, Doblin, Monika S., Bacic, Antony, Chen, Xiao-Yang, Marchant, Alan, Deng, Xiaomei and Wu, Ai-Min (2014) Biochemical and molecular changes associated with heteroxylan biosynthesis in Neolamarckia cadamba (Rubiaceae) during xylogenesis. Frontiers in Plant Science, 5, 602. (doi:10.3389/fpls.2014.00602). (PMID:25426124)

Record type: Article

Abstract

Wood, derived from plant secondary growth, is a commercially important material. Both cellulose and lignin assembly have been well studied during wood formation (xylogenesis), but heteroxylan biosynthesis is less well defined. Elucidation of the heteroxylan biosynthetic pathway is crucial to understand the mechanism of wood formation. Here, we use Neolamarckia cadamba, a fast-growing tropical tree, as a sample to analyze heteroxylan formation at the biochemical and molecular levels during wood formation. Analysis of the non-cellulosic polysaccharides isolated from N. cadamba stems shows that heteroxylans dominate non-cellulosic polysaccharides and increase with xylogenesis. Microsomes isolated from stems of 1-year-old N. cadamba exhibited UDP-Xyl synthase and xylosyltransferase activities with the highest activity present in the middle and basal stem regions. To further understand the genetic basis of heteroxylan synthesis, RNA sequencing (RNA-seq) was used to generate transcriptomes of N. cadamba during xylogenesis. The RNA-seq results showed that genes related to heteroxylan synthesis had higher expression levels in the middle and basal part of the stem compared to the apical part. Our results describe the heteroxylan distribution and heteroxylan synthesis trait in N. cadamba and give a new example for understanding the mechanism of heteroxylan synthesis in tropical tree species in future

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Accepted/In Press date: 16 October 2014
Published date: 7 November 2014
Organisations: Centre for Biological Sciences

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Local EPrints ID: 377481
URI: http://eprints.soton.ac.uk/id/eprint/377481
ISSN: 1664-462X
PURE UUID: f957941f-5534-47f8-9bdf-683662f07583

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Date deposited: 08 Jun 2015 12:30
Last modified: 16 Sep 2019 18:28

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Contributors

Author: Xianhai Zhao
Author: Kunxi Ouyang
Author: Siming Gan
Author: Wei Zeng
Author: Lili Song
Author: Shuai Zhao
Author: Juncheng Li
Author: Monika S. Doblin
Author: Antony Bacic
Author: Xiao-Yang Chen
Author: Alan Marchant
Author: Xiaomei Deng
Author: Ai-Min Wu

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