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Biorealistic cardiac cell culture platforms with integrated monitoring of extracellular action potentials

Biorealistic cardiac cell culture platforms with integrated monitoring of extracellular action potentials
Biorealistic cardiac cell culture platforms with integrated monitoring of extracellular action potentials
Current platforms for in vitro drug development utilize confluent, unorganized monolayers of heart cells to study the effect on action potential propagation. However, standard cell cultures are of limited use in cardiac research, as they do not preserve important structural and functional properties of the myocardium. Here we present a method to integrate a scaffolding technology with multi-electrode arrays and deliver a compact, off-the-shelf monitoring platform for growing biomimetic cardiac tissue. Our approach produces anisotropic cultures with conduction velocity (CV) profiles that closer resemble native heart tissue; the fastest impulse propagation is along the long axis of the aligned cardiomyocytes (CVL) and the slowest propagation is perpendicular (CVT), in contrast to standard cultures where action potential propagates isotropically (CVL???CVT). The corresponding anisotropy velocity ratios (CVL/CVT?=?1.38?–?2.22) are comparable with values for healthy adult rat ventricles (1.98?–?3.63). The main advantages of this approach are that (i) it provides ultimate pattern control, (ii) it is compatible with automated manufacturing steps and (iii) it is utilized through standard cell culturing protocols. Our platform is compatible with existing read-out equipment and comprises a prompt method for more reliable CV studies.
1-13
Trantidou, T.
1a2705ad-366d-432b-be5f-523d1303930c
Terracciano, C.M.
79bb2862-f4c4-42b1-929c-3043b5bc8794
Kontziampasis, D.
0bffea5c-c766-4f82-b153-e5c00ac3df33
Humphrey, E.J.
d728a181-61c5-4b44-9783-e94c6c95a5c6
Prodromakis, T.
d58c9c10-9d25-4d22-b155-06c8437acfbf
Trantidou, T.
1a2705ad-366d-432b-be5f-523d1303930c
Terracciano, C.M.
79bb2862-f4c4-42b1-929c-3043b5bc8794
Kontziampasis, D.
0bffea5c-c766-4f82-b153-e5c00ac3df33
Humphrey, E.J.
d728a181-61c5-4b44-9783-e94c6c95a5c6
Prodromakis, T.
d58c9c10-9d25-4d22-b155-06c8437acfbf

Trantidou, T., Terracciano, C.M., Kontziampasis, D., Humphrey, E.J. and Prodromakis, T. (2015) Biorealistic cardiac cell culture platforms with integrated monitoring of extracellular action potentials. Scientific Reports, 5 (11067), 1-13. (doi:10.1038/srep11067). (PMID:26053434)

Record type: Article

Abstract

Current platforms for in vitro drug development utilize confluent, unorganized monolayers of heart cells to study the effect on action potential propagation. However, standard cell cultures are of limited use in cardiac research, as they do not preserve important structural and functional properties of the myocardium. Here we present a method to integrate a scaffolding technology with multi-electrode arrays and deliver a compact, off-the-shelf monitoring platform for growing biomimetic cardiac tissue. Our approach produces anisotropic cultures with conduction velocity (CV) profiles that closer resemble native heart tissue; the fastest impulse propagation is along the long axis of the aligned cardiomyocytes (CVL) and the slowest propagation is perpendicular (CVT), in contrast to standard cultures where action potential propagates isotropically (CVL???CVT). The corresponding anisotropy velocity ratios (CVL/CVT?=?1.38?–?2.22) are comparable with values for healthy adult rat ventricles (1.98?–?3.63). The main advantages of this approach are that (i) it provides ultimate pattern control, (ii) it is compatible with automated manufacturing steps and (iii) it is utilized through standard cell culturing protocols. Our platform is compatible with existing read-out equipment and comprises a prompt method for more reliable CV studies.

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More information

Accepted/In Press date: 13 May 2015
Published date: 8 June 2015
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 377542
URI: https://eprints.soton.ac.uk/id/eprint/377542
PURE UUID: 11c0fd10-d55c-4f39-a6bf-8438bb595ed0
ORCID for T. Prodromakis: ORCID iD orcid.org/0000-0002-6267-6909

Catalogue record

Date deposited: 09 Jun 2015 11:05
Last modified: 06 Jun 2018 12:24

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