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Investigating biomechanical noise in neuroblastoma cells using the quartz crystal microbalance

Investigating biomechanical noise in neuroblastoma cells using the quartz crystal microbalance
Investigating biomechanical noise in neuroblastoma cells using the quartz crystal microbalance
Quantifying cellular behaviour by motility and morphology changes is increasingly important in formulating an understanding of fundamental physiological phenomena and cellular mechanisms of disease. However, cells are complex biological units, which often respond to external environmental factors by manifesting subtle responses that may be difficult to interpret using conventional biophysical measurements. This paper describes the adaptation of the quartz crystal microbalance (QCM) to monitor neuroblastoma cells undergoing environmental stress wherein the frequency stability of the device can be correlated to changes in cellular state. By employing time domain analysis of the resulting frequency fluctuations, it is possible to study the variations in cellular motility and distinguish between different cell states induced by applied external heat stress. The changes in the frequency fluctuation data are correlated to phenotypical physical response recorded using optical microscopy under identical conditions of environmental stress. This technique, by probing the associated biomechanical noise, paves the way for its use in monitoring cell activity, and intrinsic motility and morphology changes, as well as the modulation resulting from the action of drugs, toxins and environmental stress
20141389-20141389
Prasad, A.
6e22ff68-5a7e-46c7-8fd4-ab562f7e9752
Huefner, Anna
135d7fc3-aff0-4135-b98b-2c070e795ac0
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Seshia, A.A.
a2825499-d051-44ef-b545-4a312c64cf59
Prasad, A.
6e22ff68-5a7e-46c7-8fd4-ab562f7e9752
Huefner, Anna
135d7fc3-aff0-4135-b98b-2c070e795ac0
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Seshia, A.A.
a2825499-d051-44ef-b545-4a312c64cf59

Prasad, A., Huefner, Anna, Mahajan, Sumeet and Seshia, A.A. (2015) Investigating biomechanical noise in neuroblastoma cells using the quartz crystal microbalance. Journal of the Royal Society Interface, 12 (106), 20141389-20141389. (doi:10.1098/rsif.2014.1389).

Record type: Article

Abstract

Quantifying cellular behaviour by motility and morphology changes is increasingly important in formulating an understanding of fundamental physiological phenomena and cellular mechanisms of disease. However, cells are complex biological units, which often respond to external environmental factors by manifesting subtle responses that may be difficult to interpret using conventional biophysical measurements. This paper describes the adaptation of the quartz crystal microbalance (QCM) to monitor neuroblastoma cells undergoing environmental stress wherein the frequency stability of the device can be correlated to changes in cellular state. By employing time domain analysis of the resulting frequency fluctuations, it is possible to study the variations in cellular motility and distinguish between different cell states induced by applied external heat stress. The changes in the frequency fluctuation data are correlated to phenotypical physical response recorded using optical microscopy under identical conditions of environmental stress. This technique, by probing the associated biomechanical noise, paves the way for its use in monitoring cell activity, and intrinsic motility and morphology changes, as well as the modulation resulting from the action of drugs, toxins and environmental stress

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MotilityandMorphologyUsingQCM-Interface - final accepted manuscript.pdf - Accepted Manuscript
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Accepted/In Press date: 13 March 2015
Published date: 15 April 2015
Organisations: Faculty, Institute for Life Sciences, Chemical Biology Group

Identifiers

Local EPrints ID: 378255
URI: http://eprints.soton.ac.uk/id/eprint/378255
PURE UUID: 4e3d9827-ec95-4ac4-aa42-ff4c6dcc5b91
ORCID for Sumeet Mahajan: ORCID iD orcid.org/0000-0001-8923-6666

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Date deposited: 29 Jun 2015 10:59
Last modified: 21 Nov 2021 02:59

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Contributors

Author: A. Prasad
Author: Anna Huefner
Author: Sumeet Mahajan ORCID iD
Author: A.A. Seshia

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