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Local origins impart conserved bone type-related differences in human osteoblast behaviour

Local origins impart conserved bone type-related differences in human osteoblast behaviour
Local origins impart conserved bone type-related differences in human osteoblast behaviour
Osteogenic behaviour of osteoblasts from trabecular, cortical and subchondral bone were examined to determine any bone type-selective differences in samples from both osteoarthritic (OA) and osteoporotic (OP) patients. Cell growth, differentiation; alkaline phosphatase (TNAP) mRNA and activity, Runt-related transcription factor-2 (RUNX2), SP7-transcription factor (SP7), bone sialoprotein-II (BSP-II), osteocalcin/bone gamma-carboxyglutamate (BGLAP), osteoprotegerin (OPG, TNFRSF11B), receptor activator of nuclear factor-?? ligand (RANKL, TNFSF11) mRNA levels and proangiogenic vascular endothelial growth factor-A (VEGF-A) mRNA and protein release were assessed in osteoblasts from paired humeral head samples from age-matched, human OA/OP (n = 5/4) patients. Initial outgrowth and increase in cell number were significantly faster (p < 0.01) in subchondral and cortical than trabecular osteoblasts, in OA and OP, and this bone type-related differences were conserved despite consistently faster growth in OA. RUNX2/SP7 levels and TNAP mRNA and protein activity were, however, greater in trabecular than subchondral and cortical osteoblasts in OA and OP. BSP-II levels were significantly greater in trabecular and lowest in cortical osteoblasts in both OA and OP. In contrast, BGLAP levels showed divergent bone type-selective behaviour; highest in osteoblasts from subchondral origins in OA and trabecular origins in OP. We found virtually identical bone type-related differences, however, in TNFRSF11B:TNFSF11 in OA and OP, consistent with greater potential for paracrine effects on osteoclasts in trabecular osteoblasts. Subchondral osteoblasts (OA) exhibited highest VEGF-A mRNA levels and release. Our data indicate that human osteoblasts in trabecular, subchondral and cortical bone have inherent, programmed diversity, with specific bone type-related differences in growth, differentiation and pro-angiogenic potential in vitro.
in vitro, human osteoblasts, shoulder arthroplasty, vasculature, subchondral, trabecular, cortical, osteogenic differentiation
155-176
Shah, M.
c89fdf39-3923-475c-9365-d51d86888d6c
Gburcik, V.
74e42800-3075-44f0-ba0d-01d0e5b1b290
Reilly, P.
c6b3c706-23e1-45ac-83f6-475052ee7ec2
Sankey, R.A.
3a768eec-3a62-466c-a1c7-56cd4796acfb
Emery, R.J.
5e119a17-dbd0-45a6-82b4-e47b9051ac7c
Clarkin, C.E.
05cd2a88-1127-41aa-a29b-7ac323b4f3c9
Pitsillides, A.A.
0181cd36-d160-4955-8b4b-06bc96bba25d
Shah, M.
c89fdf39-3923-475c-9365-d51d86888d6c
Gburcik, V.
74e42800-3075-44f0-ba0d-01d0e5b1b290
Reilly, P.
c6b3c706-23e1-45ac-83f6-475052ee7ec2
Sankey, R.A.
3a768eec-3a62-466c-a1c7-56cd4796acfb
Emery, R.J.
5e119a17-dbd0-45a6-82b4-e47b9051ac7c
Clarkin, C.E.
05cd2a88-1127-41aa-a29b-7ac323b4f3c9
Pitsillides, A.A.
0181cd36-d160-4955-8b4b-06bc96bba25d

Shah, M., Gburcik, V., Reilly, P., Sankey, R.A., Emery, R.J., Clarkin, C.E. and Pitsillides, A.A. (2015) Local origins impart conserved bone type-related differences in human osteoblast behaviour. European Cells & Materials, 4 (29), 155-176. (doi:10.22203/eCM). (PMID:25738584)

Record type: Article

Abstract

Osteogenic behaviour of osteoblasts from trabecular, cortical and subchondral bone were examined to determine any bone type-selective differences in samples from both osteoarthritic (OA) and osteoporotic (OP) patients. Cell growth, differentiation; alkaline phosphatase (TNAP) mRNA and activity, Runt-related transcription factor-2 (RUNX2), SP7-transcription factor (SP7), bone sialoprotein-II (BSP-II), osteocalcin/bone gamma-carboxyglutamate (BGLAP), osteoprotegerin (OPG, TNFRSF11B), receptor activator of nuclear factor-?? ligand (RANKL, TNFSF11) mRNA levels and proangiogenic vascular endothelial growth factor-A (VEGF-A) mRNA and protein release were assessed in osteoblasts from paired humeral head samples from age-matched, human OA/OP (n = 5/4) patients. Initial outgrowth and increase in cell number were significantly faster (p < 0.01) in subchondral and cortical than trabecular osteoblasts, in OA and OP, and this bone type-related differences were conserved despite consistently faster growth in OA. RUNX2/SP7 levels and TNAP mRNA and protein activity were, however, greater in trabecular than subchondral and cortical osteoblasts in OA and OP. BSP-II levels were significantly greater in trabecular and lowest in cortical osteoblasts in both OA and OP. In contrast, BGLAP levels showed divergent bone type-selective behaviour; highest in osteoblasts from subchondral origins in OA and trabecular origins in OP. We found virtually identical bone type-related differences, however, in TNFRSF11B:TNFSF11 in OA and OP, consistent with greater potential for paracrine effects on osteoclasts in trabecular osteoblasts. Subchondral osteoblasts (OA) exhibited highest VEGF-A mRNA levels and release. Our data indicate that human osteoblasts in trabecular, subchondral and cortical bone have inherent, programmed diversity, with specific bone type-related differences in growth, differentiation and pro-angiogenic potential in vitro.

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More information

Published date: 4 March 2015
Keywords: in vitro, human osteoblasts, shoulder arthroplasty, vasculature, subchondral, trabecular, cortical, osteogenic differentiation
Organisations: Biomedicine

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Local EPrints ID: 378348
URI: https://eprints.soton.ac.uk/id/eprint/378348
PURE UUID: 65df3b38-8611-4b2b-a9ac-166112773e4a

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Date deposited: 30 Jun 2015 10:14
Last modified: 15 Aug 2019 16:30

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