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Characterization of the prokaryotic sodium channel NavSp pore with a microfluidic bilayer platform

Characterization of the prokaryotic sodium channel NavSp pore with a microfluidic bilayer platform
Characterization of the prokaryotic sodium channel NavSp pore with a microfluidic bilayer platform
This paper describes the use of a newly-developed micro-chip bilayer platform to examine the electrophysiological properties of the prokaryotic voltage-gated sodium channel pore (NavSp) from Silicibacter pomeroyi. The platform allows up to 6 bilayers to be analysed simultaneously. Proteoliposomes were incorporated into suspended lipid bilayers formed within the microfluidic bilayer chips. The chips provide access to bilayers from either side, enabling the fast and controlled titration of compounds. Dose-dependent modulation of the opening probability by the channel blocking drug nifedipine was measured and its IC50 determined.
1932-6203
1-9
Saha, Shimul C.
e2c8a09a-21af-4a31-bd25-26945094e03a
Henderson, Alexander J.
f71b1eaf-66f6-494e-a4cb-d141353f6395
Powl, Andrew M.
79b77ec3-767b-42ad-991c-d843e29af8e3
Wallace, B.A.
ec92996f-4630-4e2a-a9b0-852fed8cdd9d
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Saha, Shimul C.
e2c8a09a-21af-4a31-bd25-26945094e03a
Henderson, Alexander J.
f71b1eaf-66f6-494e-a4cb-d141353f6395
Powl, Andrew M.
79b77ec3-767b-42ad-991c-d843e29af8e3
Wallace, B.A.
ec92996f-4630-4e2a-a9b0-852fed8cdd9d
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Saha, Shimul C., Henderson, Alexander J., Powl, Andrew M., Wallace, B.A., de Planque, Maurits R.R. and Morgan, Hywel (2015) Characterization of the prokaryotic sodium channel NavSp pore with a microfluidic bilayer platform. PLoS ONE, 10 (7), 1-9. (doi:10.1371/journal.pone.0131286). (PMID:26147601)

Record type: Article

Abstract

This paper describes the use of a newly-developed micro-chip bilayer platform to examine the electrophysiological properties of the prokaryotic voltage-gated sodium channel pore (NavSp) from Silicibacter pomeroyi. The platform allows up to 6 bilayers to be analysed simultaneously. Proteoliposomes were incorporated into suspended lipid bilayers formed within the microfluidic bilayer chips. The chips provide access to bilayers from either side, enabling the fast and controlled titration of compounds. Dose-dependent modulation of the opening probability by the channel blocking drug nifedipine was measured and its IC50 determined.

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Accepted/In Press date: 27 April 2015
Published date: 6 July 2015
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 379187
URI: https://eprints.soton.ac.uk/id/eprint/379187
ISSN: 1932-6203
PURE UUID: e6718b8b-862e-4dfd-b5df-3b34219c0876
ORCID for Maurits R.R. de Planque: ORCID iD orcid.org/0000-0002-8787-0513
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 15 Jul 2015 11:43
Last modified: 20 Jul 2019 00:59

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Contributors

Author: Shimul C. Saha
Author: Alexander J. Henderson
Author: Andrew M. Powl
Author: B.A. Wallace
Author: Hywel Morgan ORCID iD

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