Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs
Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs
The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM. The virus employed in this model is based on an engineered vector incapable of replication after initial infection. We show that despite this strategy, replication competent viral particles are released into the culture medium due to recombination between overlapping sequences of the env deleted HIV genome that is co-transfected with intact env. This finding emphasizes the need for careful data analysis and interpretation if similar constructs are employed and urges for additional caution during laboratory work.
70
Bonczkowski, P.
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De Spiegelaere, W.
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Bosque, A.
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White, C.H.
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Van Nuffel, A.
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Malatinkova, E.
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Kiselinova, M.
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Trypsteen, W.
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Witkowski, W.
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Vermeire, J.
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Verhasselt, B.
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Martins, L.
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Woelk, C.H.
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Planelles, V.
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Vndekerckhove, L.
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21 August 2014
Bonczkowski, P.
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De Spiegelaere, W.
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Bosque, A.
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White, C.H.
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Van Nuffel, A.
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Malatinkova, E.
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Kiselinova, M.
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Trypsteen, W.
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Witkowski, W.
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Vermeire, J.
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Verhasselt, B.
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Martins, L.
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Woelk, C.H.
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Planelles, V.
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Vndekerckhove, L.
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Bonczkowski, P., De Spiegelaere, W., Bosque, A., White, C.H., Van Nuffel, A., Malatinkova, E., Kiselinova, M., Trypsteen, W., Witkowski, W., Vermeire, J., Verhasselt, B., Martins, L., Woelk, C.H., Planelles, V. and Vndekerckhove, L.
(2014)
Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs.
Retrovirology, 11 (70), .
(doi:10.1186/s12977-014-0070-3).
(PMID:25142072)
Abstract
The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM. The virus employed in this model is based on an engineered vector incapable of replication after initial infection. We show that despite this strategy, replication competent viral particles are released into the culture medium due to recombination between overlapping sequences of the env deleted HIV genome that is co-transfected with intact env. This finding emphasizes the need for careful data analysis and interpretation if similar constructs are employed and urges for additional caution during laboratory work.
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Accepted/In Press date: 31 July 2014
e-pub ahead of print date: 21 August 2014
Published date: 21 August 2014
Organisations:
Human Development & Health, Clinical & Experimental Sciences
Identifiers
Local EPrints ID: 379204
URI: http://eprints.soton.ac.uk/id/eprint/379204
ISSN: 1742-4690
PURE UUID: a6e892a8-0b5e-4772-b1c0-558fe443f1a5
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Date deposited: 18 Jul 2015 14:25
Last modified: 14 Mar 2024 20:35
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Contributors
Author:
P. Bonczkowski
Author:
W. De Spiegelaere
Author:
A. Bosque
Author:
C.H. White
Author:
A. Van Nuffel
Author:
E. Malatinkova
Author:
M. Kiselinova
Author:
W. Trypsteen
Author:
W. Witkowski
Author:
J. Vermeire
Author:
B. Verhasselt
Author:
L. Martins
Author:
C.H. Woelk
Author:
V. Planelles
Author:
L. Vndekerckhove
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