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Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs

Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs
Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs
The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM. The virus employed in this model is based on an engineered vector incapable of replication after initial infection. We show that despite this strategy, replication competent viral particles are released into the culture medium due to recombination between overlapping sequences of the env deleted HIV genome that is co-transfected with intact env. This finding emphasizes the need for careful data analysis and interpretation if similar constructs are employed and urges for additional caution during laboratory work.
1742-4690
70
Bonczkowski, P.
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De Spiegelaere, W.
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Bosque, A.
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White, C.H.
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Van Nuffel, A.
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Malatinkova, E.
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Kiselinova, M.
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Trypsteen, W.
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Witkowski, W.
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Vermeire, J.
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Verhasselt, B.
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Martins, L.
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Woelk, C.H.
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Planelles, V.
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Vndekerckhove, L.
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Bonczkowski, P.
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De Spiegelaere, W.
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Bosque, A.
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White, C.H.
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Van Nuffel, A.
8945ec59-945c-483e-8a2a-74e989c6ea53
Malatinkova, E.
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Kiselinova, M.
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Trypsteen, W.
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Witkowski, W.
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Vermeire, J.
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Verhasselt, B.
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Martins, L.
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Woelk, C.H.
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Planelles, V.
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Vndekerckhove, L.
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Bonczkowski, P., De Spiegelaere, W., Bosque, A., White, C.H., Van Nuffel, A., Malatinkova, E., Kiselinova, M., Trypsteen, W., Witkowski, W., Vermeire, J., Verhasselt, B., Martins, L., Woelk, C.H., Planelles, V. and Vndekerckhove, L. (2014) Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs. Retrovirology, 11 (70), 70. (doi:10.1186/s12977-014-0070-3). (PMID:25142072)

Record type: Article

Abstract

The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM. The virus employed in this model is based on an engineered vector incapable of replication after initial infection. We show that despite this strategy, replication competent viral particles are released into the culture medium due to recombination between overlapping sequences of the env deleted HIV genome that is co-transfected with intact env. This finding emphasizes the need for careful data analysis and interpretation if similar constructs are employed and urges for additional caution during laboratory work.

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More information

Accepted/In Press date: 31 July 2014
e-pub ahead of print date: 21 August 2014
Published date: 21 August 2014
Organisations: Human Development & Health, Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 379204
URI: https://eprints.soton.ac.uk/id/eprint/379204
ISSN: 1742-4690
PURE UUID: a6e892a8-0b5e-4772-b1c0-558fe443f1a5

Catalogue record

Date deposited: 18 Jul 2015 14:25
Last modified: 02 Jul 2018 16:31

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