The University of Southampton
University of Southampton Institutional Repository

Are you also what your mother eats? Distinct proteomic portrait as a result of maternal high-fat diet in the cerebral cortex of the adult mouse

Are you also what your mother eats? Distinct proteomic portrait as a result of maternal high-fat diet in the cerebral cortex of the adult mouse
Are you also what your mother eats? Distinct proteomic portrait as a result of maternal high-fat diet in the cerebral cortex of the adult mouse
Epidemiological studies suggest an association between maternal obesity and adverse neurodevelopmental outcomes in offspring. Our aim was to compare the global proteomic portrait in the cerebral cortex between mice born to mothers on a high-fat or control diet who themselves were fed a high-fat or control diet. Male mice born to dams fed a control (C) or high-fat (H) diet 4 weeks before conception and during gestation, and lactation were assigned to either C or H diet at weaning. Mice were killed at 19 weeks and their cerebral cortices were analysed using a two-dimensional liquid chromatography-mass spectrometry methodology. In total, 6?695 proteins were identified (q<0.01), 10% of which were modulated in at least one of the groups relative to controls. In silico analysis revealed that mice clustered based on the diet of the mother and not their own diet and that maternal high-fat diet was significantly associated with response to hypoxia/oxidative stress and apoptosis in the cerebral cortex of the adult offspring. Maternal high-fat diet resulted in distinct endophenotypic changes of the adult offspring cerebral cortex independent of its current diet. The identified proteins could represent novel therapeutic targets for the prevention of neuropathological features resulting from maternal obesity.
0307-0565
Manousopoulou, A.
ba55d059-97b3-4981-9ced-7ed3b6284be0
Woo, J.
d919a72d-6b27-4f79-98e7-f68ffc51248a
Woelk, C.H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Johnston, H.
8d4500ff-fec8-47ef-b766-798689948cd6
Singhania, A.
9a5f2c6b-fc46-4223-bcae-de70bf14da6b
Hawkes, C.
1afc9e85-d3d9-4daf-b0fd-b508e60817f2
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Carare, Roxana-Octavia
0478c197-b0c1-4206-acae-54e88c8f21fa
Manousopoulou, A.
ba55d059-97b3-4981-9ced-7ed3b6284be0
Woo, J.
d919a72d-6b27-4f79-98e7-f68ffc51248a
Woelk, C.H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Johnston, H.
8d4500ff-fec8-47ef-b766-798689948cd6
Singhania, A.
9a5f2c6b-fc46-4223-bcae-de70bf14da6b
Hawkes, C.
1afc9e85-d3d9-4daf-b0fd-b508e60817f2
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Carare, Roxana-Octavia
0478c197-b0c1-4206-acae-54e88c8f21fa

Manousopoulou, A., Woo, J., Woelk, C.H., Johnston, H., Singhania, A., Hawkes, C., Garbis, Spiros D. and Carare, Roxana-Octavia (2015) Are you also what your mother eats? Distinct proteomic portrait as a result of maternal high-fat diet in the cerebral cortex of the adult mouse. International Journal of Obesity. (doi:10.1038/ijo.2015.35).

Record type: Article

Abstract

Epidemiological studies suggest an association between maternal obesity and adverse neurodevelopmental outcomes in offspring. Our aim was to compare the global proteomic portrait in the cerebral cortex between mice born to mothers on a high-fat or control diet who themselves were fed a high-fat or control diet. Male mice born to dams fed a control (C) or high-fat (H) diet 4 weeks before conception and during gestation, and lactation were assigned to either C or H diet at weaning. Mice were killed at 19 weeks and their cerebral cortices were analysed using a two-dimensional liquid chromatography-mass spectrometry methodology. In total, 6?695 proteins were identified (q<0.01), 10% of which were modulated in at least one of the groups relative to controls. In silico analysis revealed that mice clustered based on the diet of the mother and not their own diet and that maternal high-fat diet was significantly associated with response to hypoxia/oxidative stress and apoptosis in the cerebral cortex of the adult offspring. Maternal high-fat diet resulted in distinct endophenotypic changes of the adult offspring cerebral cortex independent of its current diet. The identified proteins could represent novel therapeutic targets for the prevention of neuropathological features resulting from maternal obesity.

PDF
ijo201535a.pdf - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 6 January 2015
e-pub ahead of print date: 23 March 2015
Published date: 21 April 2015
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 379215
URI: https://eprints.soton.ac.uk/id/eprint/379215
ISSN: 0307-0565
PURE UUID: 384956d9-139c-4e28-a535-58b002046a5e
ORCID for Spiros D. Garbis: ORCID iD orcid.org/0000-0002-1050-0805

Catalogue record

Date deposited: 18 Jul 2015 15:08
Last modified: 07 Jun 2019 00:32

Export record

Altmetrics

Contributors

Author: A. Manousopoulou
Author: J. Woo
Author: C.H. Woelk
Author: H. Johnston
Author: A. Singhania
Author: C. Hawkes

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×