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Stretching of single DNA molecules under pressure-driven flow in straight and curved microfluidic channels

Stretching of single DNA molecules under pressure-driven flow in straight and curved microfluidic channels
Stretching of single DNA molecules under pressure-driven flow in straight and curved microfluidic channels
Microfluidic channels are investigated here with a view to unravel and deliver long genomic DNA strands (>20µm) into channels for subsequent optical interrogation. Previous studies have focused on devices where strands are stretched under sudden shear and elongational microflows, but unfortunately there is a potential for strand fragmentation. We investigate differently shaped microchannels to deliver intact strands for subsequent genome mapping.
microfluidics, DNA mapping, fluid dynamics, brownian motion
Horak, P.
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Noual, A.
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Humphreys, T.
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Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Horak, P.
520489b5-ccc7-4d29-bb30-c1e36436ea03
Noual, A.
5c5d5d71-3417-4e61-93fa-4b2c2bdc3513
Humphreys, T.
fc0792e4-e186-4fcd-a170-0ec11732d776
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Horak, P., Noual, A., Humphreys, T. and Melvin, T. (2015) Stretching of single DNA molecules under pressure-driven flow in straight and curved microfluidic channels. Trends in Optical Micromanipulation III, Obergurgl, Austria. 25 - 30 Jan 2015. 1 pp .

Record type: Conference or Workshop Item (Paper)

Abstract

Microfluidic channels are investigated here with a view to unravel and deliver long genomic DNA strands (>20µm) into channels for subsequent optical interrogation. Previous studies have focused on devices where strands are stretched under sudden shear and elongational microflows, but unfortunately there is a potential for strand fragmentation. We investigate differently shaped microchannels to deliver intact strands for subsequent genome mapping.

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More information

Published date: January 2015
Venue - Dates: Trends in Optical Micromanipulation III, Obergurgl, Austria, 2015-01-25 - 2015-01-30
Keywords: microfluidics, DNA mapping, fluid dynamics, brownian motion
Organisations: Optoelectronics Research Centre

Identifiers

Local EPrints ID: 379317
URI: https://eprints.soton.ac.uk/id/eprint/379317
PURE UUID: b5a9d4d9-a6f8-49fd-a1e4-a716be422f3f
ORCID for P. Horak: ORCID iD orcid.org/0000-0002-8710-8764

Catalogue record

Date deposited: 21 Jul 2015 11:08
Last modified: 30 Nov 2018 01:34

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