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Phosphite utilization by the globally important marine diazotroph Trichodesmium

Phosphite utilization by the globally important marine diazotroph Trichodesmium
Phosphite utilization by the globally important marine diazotroph Trichodesmium
Species belonging to the filamentous cyanobacterial genus Trichodesmium are responsible for a significant fraction of oceanic nitrogen fixation. The availability of phosphorus (P) likely constrains the growth of Trichodesmium in certain regions of the ocean. Moreover, Trichodesmium species have recently been shown to play a role in an emerging oceanic phosphorus redox cycle, further highlighting the key role these microbes play in many biogeochemical processes in the contemporary ocean. Here, we show that Trichodesmium erythraeum?IMS101 can grow on the reduced inorganic compound phosphite as its sole source of P. The components responsible for phosphite utilization are identified through heterologous expression of the T. erythraeum?IMS101 Tery_0365–0368 genes, encoding a putative adenosine triphosphate (ATP)-binding cassette transporter and nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenase, in the model cyanobacteria Synechocystis sp. PCC6803. We demonstrate that only combined expression of both the transporter and the dehydrogenase enables Synechocystis to utilize phosphite, confirming the function of Tery_0365-0367 as a phosphite uptake system (PtxABC) and Tery_0368 as a phosphite dehydrogenase (PtxD). Our findings suggest that reported uptake of phosphite by Trichodesmium consortia in the field likely reflects an active biological process by Trichodesmium. These results highlight the diversity of phosphorus sources available to Trichodesmium in a resource-limited ocean.
1758-2229
824-830
Polyviou, Despo
7fcaf51c-0615-4967-a180-f5405f7b8070
Hitchcock, Andrew
aeca86f3-e8cf-47d1-9338-e82630fb868e
Baylay, Alison J.
08ea69f8-9910-4c8b-86a9-602e45b8a44e
Moore, C. Mark
7ec80b7b-bedc-4dd5-8924-0f5d01927b12
Bibby, Thomas S.
e04ea079-dd90-4ead-9840-00882de27ebd
Polyviou, Despo
7fcaf51c-0615-4967-a180-f5405f7b8070
Hitchcock, Andrew
aeca86f3-e8cf-47d1-9338-e82630fb868e
Baylay, Alison J.
08ea69f8-9910-4c8b-86a9-602e45b8a44e
Moore, C. Mark
7ec80b7b-bedc-4dd5-8924-0f5d01927b12
Bibby, Thomas S.
e04ea079-dd90-4ead-9840-00882de27ebd

Polyviou, Despo, Hitchcock, Andrew, Baylay, Alison J., Moore, C. Mark and Bibby, Thomas S. (2015) Phosphite utilization by the globally important marine diazotroph Trichodesmium. Environmental Microbiology Reports, 7 (6), 824-830. (doi:10.1111/1758-2229.12308).

Record type: Article

Abstract

Species belonging to the filamentous cyanobacterial genus Trichodesmium are responsible for a significant fraction of oceanic nitrogen fixation. The availability of phosphorus (P) likely constrains the growth of Trichodesmium in certain regions of the ocean. Moreover, Trichodesmium species have recently been shown to play a role in an emerging oceanic phosphorus redox cycle, further highlighting the key role these microbes play in many biogeochemical processes in the contemporary ocean. Here, we show that Trichodesmium erythraeum?IMS101 can grow on the reduced inorganic compound phosphite as its sole source of P. The components responsible for phosphite utilization are identified through heterologous expression of the T. erythraeum?IMS101 Tery_0365–0368 genes, encoding a putative adenosine triphosphate (ATP)-binding cassette transporter and nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenase, in the model cyanobacteria Synechocystis sp. PCC6803. We demonstrate that only combined expression of both the transporter and the dehydrogenase enables Synechocystis to utilize phosphite, confirming the function of Tery_0365-0367 as a phosphite uptake system (PtxABC) and Tery_0368 as a phosphite dehydrogenase (PtxD). Our findings suggest that reported uptake of phosphite by Trichodesmium consortia in the field likely reflects an active biological process by Trichodesmium. These results highlight the diversity of phosphorus sources available to Trichodesmium in a resource-limited ocean.

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More information

e-pub ahead of print date: 23 July 2015
Published date: 23 July 2015
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 379605
URI: http://eprints.soton.ac.uk/id/eprint/379605
ISSN: 1758-2229
PURE UUID: 2b5dc752-c5c1-422b-ada9-09da16d22158
ORCID for Alison J. Baylay: ORCID iD orcid.org/0000-0002-0814-0740
ORCID for C. Mark Moore: ORCID iD orcid.org/0000-0002-9541-6046

Catalogue record

Date deposited: 24 Jul 2015 09:34
Last modified: 27 Apr 2022 02:01

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Contributors

Author: Despo Polyviou
Author: Andrew Hitchcock
Author: C. Mark Moore ORCID iD
Author: Thomas S. Bibby

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