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Towards chemiluminescence detection in micro-sequential injection lab-on-valve format: A proof of concept based on the reaction between Fe(II) and luminol in seawater

Towards chemiluminescence detection in micro-sequential injection lab-on-valve format: A proof of concept based on the reaction between Fe(II) and luminol in seawater
Towards chemiluminescence detection in micro-sequential injection lab-on-valve format: A proof of concept based on the reaction between Fe(II) and luminol in seawater
Micro-sequential injection lab-on-valve (µSI-LOV) is a well-established analytical platform for absorbance and fluorescence based assays but its applicability to chemiluminescence detection remains largely unexplored. In this work, we describe a novel fluidic protocol and two distinct strategies for photon collection that enable chemiluminescence detection using µSI-LOV for the first time. To illustrate this proof of concept, we selected the reaction between Fe(II) and luminol and developed a preliminary protocol for Fe(II) determinations in acidified seawater. The optimized fluidic strategy consists of holding 100 µL of the luminol reagent in a confined zone of the LOV and then displacing it with 50 µL of sample while monitoring the chemiluminescent product. Detection is achieved using two strategies: one based on a bifurcated optical fiber and the other based on a customized detection window created by mounting a photomultiplier tube atop of the LOV device. We show that detection is possible using both strategies but that the window strategy yields significantly enhanced sensitivity (355×) due to the larger detection area. In our final experimental conditions and using window detection, it was possible to achieve a limit of detection (LOD) of 1 nmol L?1 and to quantify Fe(II) in acidified seawater samples up to 20.00 nmol L?1 with high precision (RSD<6%). These analytical features combined with the long-term stability of luminol solution and the full automation and low reagent consumption make this approach a promising analytical tool for shipboard analysis of Fe(II). The intrinsic capacity of the LOV to operate at a low microliter level and to handle solid phases also opens up a new avenue for chemiluminescence applications. Moreover, this contribution shows that LOV can be a universal platform for optical detection, capable of absorbance, fluorescence and luminescence measurements in a single instrument setup.
Chemiluminescence, Lab-on-valve, Iron, Luminol, Seawater
0039-9140
107-111
Oliveira, Hugo M.
766848b7-8a07-4dfa-a972-576cd987b46d
Grand, Maxime M.
659acbde-d639-42b7-9fab-52fa1b3655ff
Ruzicka, Jaromir
c8f7b9f2-c2c0-40b2-8e02-e992b30ef953
Measures, Christopher I.
bee0ab0a-cbec-491e-bcfa-e0cec9df67bb
Oliveira, Hugo M.
766848b7-8a07-4dfa-a972-576cd987b46d
Grand, Maxime M.
659acbde-d639-42b7-9fab-52fa1b3655ff
Ruzicka, Jaromir
c8f7b9f2-c2c0-40b2-8e02-e992b30ef953
Measures, Christopher I.
bee0ab0a-cbec-491e-bcfa-e0cec9df67bb

Oliveira, Hugo M., Grand, Maxime M., Ruzicka, Jaromir and Measures, Christopher I. (2015) Towards chemiluminescence detection in micro-sequential injection lab-on-valve format: A proof of concept based on the reaction between Fe(II) and luminol in seawater. [in special issue: 18TH ICFIA (2013)] Talanta, 133, 107-111. (doi:10.1016/j.talanta.2014.06.076).

Record type: Article

Abstract

Micro-sequential injection lab-on-valve (µSI-LOV) is a well-established analytical platform for absorbance and fluorescence based assays but its applicability to chemiluminescence detection remains largely unexplored. In this work, we describe a novel fluidic protocol and two distinct strategies for photon collection that enable chemiluminescence detection using µSI-LOV for the first time. To illustrate this proof of concept, we selected the reaction between Fe(II) and luminol and developed a preliminary protocol for Fe(II) determinations in acidified seawater. The optimized fluidic strategy consists of holding 100 µL of the luminol reagent in a confined zone of the LOV and then displacing it with 50 µL of sample while monitoring the chemiluminescent product. Detection is achieved using two strategies: one based on a bifurcated optical fiber and the other based on a customized detection window created by mounting a photomultiplier tube atop of the LOV device. We show that detection is possible using both strategies but that the window strategy yields significantly enhanced sensitivity (355×) due to the larger detection area. In our final experimental conditions and using window detection, it was possible to achieve a limit of detection (LOD) of 1 nmol L?1 and to quantify Fe(II) in acidified seawater samples up to 20.00 nmol L?1 with high precision (RSD<6%). These analytical features combined with the long-term stability of luminol solution and the full automation and low reagent consumption make this approach a promising analytical tool for shipboard analysis of Fe(II). The intrinsic capacity of the LOV to operate at a low microliter level and to handle solid phases also opens up a new avenue for chemiluminescence applications. Moreover, this contribution shows that LOV can be a universal platform for optical detection, capable of absorbance, fluorescence and luminescence measurements in a single instrument setup.

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More information

Accepted/In Press date: 15 June 2014
Published date: February 2015
Keywords: Chemiluminescence, Lab-on-valve, Iron, Luminol, Seawater
Organisations: Geochemistry

Identifiers

Local EPrints ID: 379890
URI: http://eprints.soton.ac.uk/id/eprint/379890
ISSN: 0039-9140
PURE UUID: 113f0e07-0bd3-430d-aaad-69b5ef3d3b2b
ORCID for Maxime M. Grand: ORCID iD orcid.org/0000-0001-9338-694X

Catalogue record

Date deposited: 03 Aug 2015 13:26
Last modified: 15 Jul 2019 21:10

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