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Two apple repetitive sequence elements: characterisation and potential use as genetic markers

Two apple repetitive sequence elements: characterisation and potential use as genetic markers
Two apple repetitive sequence elements: characterisation and potential use as genetic markers


Within the 5? flanking regions of two ripening-related genes from apple (Malus pumila), two short repeat sequences were identified and named Ars1 andArs2 (Apple repetitivesequences). Both elements were present in the promoter of the Md-ACS1-1 ACC synthase gene and Ars2was also found in the promoter of a gene homologous to the ABG1 ?-galactosidase gene. Database searches revealed other examples of each element in apple genomic sequences and a partial Ars2element in a sequence from Asian pear (Pyrus pyrifolia). DNA dot blot analysis demonstrated that both elements are present in high numbers in the apple genome. Ars1 is thought to represent a MITEminiature inverted-repeat transposable element) related to the MITE Alien, while the identity ofArs2, though it is undoubtedly a transposable element, remains undetermined. DNA dot blot analysis and PCR amplification using primers designed from Ars1and Ars2 showed that elements similar to Ars1 and Ars2 are present across the Rosaceae. The amplification patterns obtained using Ars1- and Ars2-specific primers with genomic DNA from different cultivars or mapping populations suggest that these elements could be used for finger printing and segregation analysis in Rosaceae. Evidence from DNA database searches suggests that they may be applicable to genetic studies in a wide range of other organisms.
apple, fingerprinting, genetic markers, Rosaceae, transposable elements
0014-2336
177-187
Hadonou, A.M.
5476f2ca-1f5a-4330-bf80-fca732fda98d
Gittins, J.R.
c4d269cc-aae0-4182-bc81-78dc724f7d95
Hiles, E.R.
96d62a46-66a8-48d8-a8d7-722778d46e6c
James, J.D.
e670d7a5-2cfa-47a2-8b4f-f569ca95c26e
Hadonou, A.M.
5476f2ca-1f5a-4330-bf80-fca732fda98d
Gittins, J.R.
c4d269cc-aae0-4182-bc81-78dc724f7d95
Hiles, E.R.
96d62a46-66a8-48d8-a8d7-722778d46e6c
James, J.D.
e670d7a5-2cfa-47a2-8b4f-f569ca95c26e

Hadonou, A.M., Gittins, J.R., Hiles, E.R. and James, J.D. (2003) Two apple repetitive sequence elements: characterisation and potential use as genetic markers. Euphytica, 131 (2), 177-187. (doi:10.1023/A:1023927230958).

Record type: Article

Abstract



Within the 5? flanking regions of two ripening-related genes from apple (Malus pumila), two short repeat sequences were identified and named Ars1 andArs2 (Apple repetitivesequences). Both elements were present in the promoter of the Md-ACS1-1 ACC synthase gene and Ars2was also found in the promoter of a gene homologous to the ABG1 ?-galactosidase gene. Database searches revealed other examples of each element in apple genomic sequences and a partial Ars2element in a sequence from Asian pear (Pyrus pyrifolia). DNA dot blot analysis demonstrated that both elements are present in high numbers in the apple genome. Ars1 is thought to represent a MITEminiature inverted-repeat transposable element) related to the MITE Alien, while the identity ofArs2, though it is undoubtedly a transposable element, remains undetermined. DNA dot blot analysis and PCR amplification using primers designed from Ars1and Ars2 showed that elements similar to Ars1 and Ars2 are present across the Rosaceae. The amplification patterns obtained using Ars1- and Ars2-specific primers with genomic DNA from different cultivars or mapping populations suggest that these elements could be used for finger printing and segregation analysis in Rosaceae. Evidence from DNA database searches suggests that they may be applicable to genetic studies in a wide range of other organisms.

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More information

Published date: 2003
Keywords: apple, fingerprinting, genetic markers, Rosaceae, transposable elements
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 380341
URI: http://eprints.soton.ac.uk/id/eprint/380341
ISSN: 0014-2336
PURE UUID: 84c76457-03c9-44e9-a4c4-553dc07cfc1a

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Date deposited: 12 Aug 2015 15:03
Last modified: 14 Mar 2024 20:58

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Contributors

Author: A.M. Hadonou
Author: J.R. Gittins
Author: E.R. Hiles
Author: J.D. James

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