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Using heterologous and homologous promoters to study tissue specific transgene expression in fruit crops

Using heterologous and homologous promoters to study tissue specific transgene expression in fruit crops
Using heterologous and homologous promoters to study tissue specific transgene expression in fruit crops
It is desirable that the expression of transgenes in genetically improved crops is restricted to the tissue requiring the encoded activity. To this end, the ability of several heterologous and homologous gene promoters to drive expression of the ?-glucuronidase (gusA) marker gene in the vegetative tissues of transgenic apple (Malus pumila Mill.) and commercial strawberry (Fragaria x ananassa) was tested. These promoters originally drove expression in leaves (Rubisco) small subunit (SSU), RBCS3CP from tomato, SRS1P from soybean, vascular tissue, rolCP, and CoYMVP and tissue root, extAP (Brassica) and PsMTAP (Pisum). Homologous promoters from apple were cloned for expression in fruit and from strawberry for expression in stamens, petals and roots. Transgenic lines were produced by Agrobacterium-mediated transformation and the level of gusA expression in the vegetative tissues of young plants was compared with that using the CaMV 35S promoter. Quantitative GUS data were related to the copy number of transgene loci assessed by Southern blotting. The precise location of GUS activity in each tissue was identified by staining of whole leaves and tissue sections with the chromogenic substrate X-Gluc. Light-regulation and patterns of expression are recorderd in various vegetative tissues of apple.
Transgenic fruit, apple, strawberry, tissue-specific expression, gusA gene, heterologous, homologous promoters
560
55-62
International Society for Horticultural Science
James, D.J.
2c41d97c-8486-4379-b253-e5896572e81a
Gittins, J.R.
c4d269cc-aae0-4182-bc81-78dc724f7d95
Massiah, A.J.
1b0b1ba4-b70f-4ab4-bd1c-b8bc45890197
Pellny, T.K.
a8fb9a4f-dac2-4dae-89aa-461424893847
Hiles, E.R
3f8fd757-ce2f-4611-afad-20ab84225777
Biricolti, S.
9c5e9011-40a4-4ad5-bbb1-c7e54aedc460
Passey, A.J.
317f1697-2dfd-4018-a9ab-1161e93420fc
Vaughan, S.P.
8ddf1b4e-81cc-4374-b495-469dc9cf75fe
Sorvari, S.
Karhu, S.
Kanervo, E.
Pihakaski, S.
James, D.J.
2c41d97c-8486-4379-b253-e5896572e81a
Gittins, J.R.
c4d269cc-aae0-4182-bc81-78dc724f7d95
Massiah, A.J.
1b0b1ba4-b70f-4ab4-bd1c-b8bc45890197
Pellny, T.K.
a8fb9a4f-dac2-4dae-89aa-461424893847
Hiles, E.R
3f8fd757-ce2f-4611-afad-20ab84225777
Biricolti, S.
9c5e9011-40a4-4ad5-bbb1-c7e54aedc460
Passey, A.J.
317f1697-2dfd-4018-a9ab-1161e93420fc
Vaughan, S.P.
8ddf1b4e-81cc-4374-b495-469dc9cf75fe
Sorvari, S.
Karhu, S.
Kanervo, E.
Pihakaski, S.

James, D.J., Gittins, J.R., Massiah, A.J., Pellny, T.K., Hiles, E.R, Biricolti, S., Passey, A.J. and Vaughan, S.P. (2001) Using heterologous and homologous promoters to study tissue specific transgene expression in fruit crops. In, Sorvari, S., Karhu, S., Kanervo, E. and Pihakaski, S. (eds.) Acta Horticulturae IV International Symposium on In Vitro Culture and Horticultural Breeding. (Acta Horticulturae, 560) Leuven, BE. International Society for Horticultural Science, pp. 55-62.

Record type: Book Section

Abstract

It is desirable that the expression of transgenes in genetically improved crops is restricted to the tissue requiring the encoded activity. To this end, the ability of several heterologous and homologous gene promoters to drive expression of the ?-glucuronidase (gusA) marker gene in the vegetative tissues of transgenic apple (Malus pumila Mill.) and commercial strawberry (Fragaria x ananassa) was tested. These promoters originally drove expression in leaves (Rubisco) small subunit (SSU), RBCS3CP from tomato, SRS1P from soybean, vascular tissue, rolCP, and CoYMVP and tissue root, extAP (Brassica) and PsMTAP (Pisum). Homologous promoters from apple were cloned for expression in fruit and from strawberry for expression in stamens, petals and roots. Transgenic lines were produced by Agrobacterium-mediated transformation and the level of gusA expression in the vegetative tissues of young plants was compared with that using the CaMV 35S promoter. Quantitative GUS data were related to the copy number of transgene loci assessed by Southern blotting. The precise location of GUS activity in each tissue was identified by staining of whole leaves and tissue sections with the chromogenic substrate X-Gluc. Light-regulation and patterns of expression are recorderd in various vegetative tissues of apple.

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More information

Published date: 2001
Keywords: Transgenic fruit, apple, strawberry, tissue-specific expression, gusA gene, heterologous, homologous promoters
Organisations: Ocean and Earth Science

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Local EPrints ID: 380347
URI: https://eprints.soton.ac.uk/id/eprint/380347
PURE UUID: d9b1ffa1-1710-4992-b212-c2f7f9b18c81

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Date deposited: 12 Aug 2015 15:36
Last modified: 20 Sep 2018 16:31

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Contributors

Author: D.J. James
Author: J.R. Gittins
Author: A.J. Massiah
Author: T.K. Pellny
Author: E.R Hiles
Author: S. Biricolti
Author: A.J. Passey
Author: S.P. Vaughan
Editor: S. Sorvari
Editor: S. Karhu
Editor: E. Kanervo
Editor: S. Pihakaski

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