The University of Southampton
University of Southampton Institutional Repository

Determination of phytochelatins and glutathione in phytoplankton from natural waters using HPLC with fluorescence detection

Kawakami, S.K., Gledhill, M. and Achterberg, E.P. (2006) Determination of phytochelatins and glutathione in phytoplankton from natural waters using HPLC with fluorescence detection Trends in Analytical Chemistry, 25, (2), pp. 133-142. (doi:10.1016/j.trac.2005.06.005).

Record type: Article

Abstract

Phytochelatins (PCs) and glutathione (GSH) are –SH-containing compounds produced by a range of organisms for intracellular functions, such as protection against oxidative stress, metal detoxification and regulation of intracellular metal concentrations. These compounds, particularly PCs, have a potential use as metal-stress indicators for phytoplankton in natural waters. Despite their important roles, there is a paucity of data on intracellular GSH and PCs produced by natural phytoplankton assemblages. Current analytical methods for the determination of these compounds in phytoplankton from natural waters are based on high-performance liquid chromatography (HPLC) with detection of fluorescent derivatives, and comprise multi-step protocols. In this article, we discuss the analytical methods for HPLC determination of PCs and GSH, as there are limitations and practical challenges when they are applied to environmental studies.

Full text not available from this repository.

More information

Published date: 2006
Keywords: glutathione, HPLC, metal detoxification, phytochelatin, thiol

Identifiers

Local EPrints ID: 38077
URI: http://eprints.soton.ac.uk/id/eprint/38077
ISSN: 0165-9936
PURE UUID: a93015a6-1344-47e1-bf2b-8dc67dc4d923

Catalogue record

Date deposited: 01 Jun 2006
Last modified: 17 Jul 2017 15:40

Export record

Altmetrics

Contributors

Author: S.K. Kawakami
Author: M. Gledhill
Author: E.P. Achterberg

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×