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Effects of metal combinations on the production of phytochelatins and glutathione by the marine diatom Phaeodactylum tricornutum

Kawakami, S.K., Gledhill, M. and Achterberg, E.P. (2006) Effects of metal combinations on the production of phytochelatins and glutathione by the marine diatom Phaeodactylum tricornutum BioMetals, 19, (1), pp. 51-60. (doi:10.1007/s10534-005-5115-6).

Record type: Article

Abstract

Copper, Cd and Zn can be found at elevated concentrations in contaminated estuarine and coastal waters and have potential toxic effects on phytoplankton species. In this study, the effects of these metals on the intracellular production of the polypeptides phytochelatin and glutathione by the marine diatom Phaeodactylum tricornutum were examined in laboratory cultures. Single additions of Cu and Cd (0.4 ?M Cu2 and 0.45 ?M Cd2+) to the culture medium induced the production of short-chained phytochelatins ((?-Glu-Cys)n-Gly where n = 2–5), whereas a single addition of Zn (2.2 ?M Zn2+) did not stimulate phytochelatin production. Combination of Zn with Cu resulted in a similar phytochelatin production compared with a single Cu addition. The simultaneous exposure to Zn and Cd led to an antagonistic effect on phytochelatin production, which was probably caused by metal competition for cellular binding sites. Glutathione concentrations were affected only upon exposure to Cd (85% increase) or the combination of Cd with Zn (65% decrease), relative to the control experiment. Ratios of phytochelatins to glutathione indicated a pronounced metal stress in response to exposures to Cu or Cd combined with Zn. This study indicates that variabilities in phytochelatin and glutathione production in the field can be explained in part by metal competition for cellular binding sites.

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Published date: 2006
Keywords: glutathione, metal toxicity, metal stress, Phaeodactylum tricornutum, phytochelatins, thiols

Identifiers

Local EPrints ID: 38082
URI: http://eprints.soton.ac.uk/id/eprint/38082
ISSN: 0966-0844
PURE UUID: ec4c90f5-fb6b-4866-9af0-0a14e7c29f06

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Date deposited: 01 Jun 2006
Last modified: 17 Jul 2017 15:40

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Contributors

Author: S.K. Kawakami
Author: M. Gledhill
Author: E.P. Achterberg

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