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Effect of model ligands on iron redox speciation in natural waters using flow injection with luminol chemiluminescence detection

Ussher, S.J., Yaqoob, M., Achterberg, E.P., Nabi, A. and Worsfold, P.J. (2005) Effect of model ligands on iron redox speciation in natural waters using flow injection with luminol chemiluminescence detection Analytical Chemistry, 77, (7), pp. 1971-1978. (doi:10.1021/ac048850a).

Record type: Article


The effects of dissolved organic compounds on the determination of nanomolar concentrations of Fe(II) have been compared using two luminol-based flow injection chemiluminescence (FI-CL) methods. One used the direct injection of sample into the luminol reagent stream, and the other incorporated on-line solid-phase extraction of the analyte on an 8-hydroxyquinoline microcolumn. The CL signals from analyses of dissolved iron species (Fe(II) and Fe(III)) with model ligands and organic compounds were examined in high-purity water and seawater. The organic compounds included natural reducing agents (e.g., ascorbic acid), nitrogen -donor/-acceptor compounds (e.g., 1,4-dipyridine, protoporphyrin IX), aromatic compounds (e.g., 1,4-dihydroxybenzene), synthetic iron chelators (e.g., EDTA), and natural iron binding compounds (e.g., desferrioxamine B, ferrichrome A). Fe(II) determinations for both luminol FI-CL methods were affected by submicromolar concentrations of redox-active compounds, strong iron binding ligands (i.e., log KFeL > 6), and compounds with electron-donating functional groups in both high-purity water and seawater. This was due to reactions between organic molecules and iron species before and during analysis, rather than chemiluminescence caused by the individual organic compounds. In addition, the effects of strong ligands and size speciation on Fe(II) recoveries from seawater following acidification (pH 2) and reduction (100 M sodium sulfite) were investigated.

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Published date: April 2005


Local EPrints ID: 38095
ISSN: 0003-2700
PURE UUID: 96785a9b-01f4-430b-9120-bd5a47f323d6

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Date deposited: 01 Jun 2006
Last modified: 17 Jul 2017 15:40

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Author: S.J. Ussher
Author: M. Yaqoob
Author: E.P. Achterberg
Author: A. Nabi
Author: P.J. Worsfold

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