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Penetration of gold nanoparticles through the skin

Penetration of gold nanoparticles through the skin
Penetration of gold nanoparticles through the skin
The assessment of nanoparticle penetration through skin is of increasing importance not only to evaluate the toxicity associated with occupational or environmental exposure to nanoparticles, but also to design rules for the fabrication of new types of transdermal drug delivery or diagnostics
approaches. While these have been the subject of much research, the lack of a systematic approach in the penetration experiments has created controversial results regarding whether nanoparticles do or not penetrate the skin.

The aim of the research presented in the thesis is to investigate the penetration of gold nanoparticles through human and mouse skin, focusing on the effect of surface charge, morphology and specific functionalisation. To study this, a penetration protocol using organ culture was designed and tested to assure the maintenance of the skin integrity in the course of our experiments. Skin samples incubated with gold nanoparticles were characterized for penetration by NPs using inductively coupled plasma atomic emission spectroscopy, transmission electron microscopy, energy-dispersive Xray spectroscopy and photoluminescence microscopy. Furthermore, epithelial cell monolayers were exposed to the gold nanoparticles to evaluate the transport through the cellular barrier. Transmission electron microscopy, light microscopy and trans epithelial electric resistance were used to characterise the cell monolayers exposed to gold nanoparticles.

Results obtained are important to enhance our understanding of the interaction of gold nanoparticles with skin, providing valuable information for the design of new nanoparticle-based transdermal delivery systems.
Fernandes, Rute Fabiana Martins
848317d8-1eb0-48ad-aefc-012ab19d9f52
Fernandes, Rute Fabiana Martins
848317d8-1eb0-48ad-aefc-012ab19d9f52
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7

Fernandes, Rute Fabiana Martins (2014) Penetration of gold nanoparticles through the skin. University of Southampton, Physical Sciences and Engineering, Doctoral Thesis, 180pp.

Record type: Thesis (Doctoral)

Abstract

The assessment of nanoparticle penetration through skin is of increasing importance not only to evaluate the toxicity associated with occupational or environmental exposure to nanoparticles, but also to design rules for the fabrication of new types of transdermal drug delivery or diagnostics
approaches. While these have been the subject of much research, the lack of a systematic approach in the penetration experiments has created controversial results regarding whether nanoparticles do or not penetrate the skin.

The aim of the research presented in the thesis is to investigate the penetration of gold nanoparticles through human and mouse skin, focusing on the effect of surface charge, morphology and specific functionalisation. To study this, a penetration protocol using organ culture was designed and tested to assure the maintenance of the skin integrity in the course of our experiments. Skin samples incubated with gold nanoparticles were characterized for penetration by NPs using inductively coupled plasma atomic emission spectroscopy, transmission electron microscopy, energy-dispersive Xray spectroscopy and photoluminescence microscopy. Furthermore, epithelial cell monolayers were exposed to the gold nanoparticles to evaluate the transport through the cellular barrier. Transmission electron microscopy, light microscopy and trans epithelial electric resistance were used to characterise the cell monolayers exposed to gold nanoparticles.

Results obtained are important to enhance our understanding of the interaction of gold nanoparticles with skin, providing valuable information for the design of new nanoparticle-based transdermal delivery systems.

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More information

Published date: September 2014
Organisations: University of Southampton, Physics & Astronomy

Identifiers

Local EPrints ID: 381281
URI: http://eprints.soton.ac.uk/id/eprint/381281
PURE UUID: 73eb9ad6-3be1-49b6-98f4-512a347533c3
ORCID for Antonios Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 13 Oct 2015 13:45
Last modified: 06 Jun 2018 12:37

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