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Fatty acid profile of plasma NEFA does not reflect adipose tissue fatty acid profile

Fatty acid profile of plasma NEFA does not reflect adipose tissue fatty acid profile
Fatty acid profile of plasma NEFA does not reflect adipose tissue fatty acid profile
Adipose tissue (AT) fatty acid (FA) composition partly reflects habitual dietary intake. Circulating NEFA are mobilised from AT and might act as a minimally invasive surrogate marker of AT FA profile. Agreement between twenty-eight FA in AT and plasma NEFA was assessed using concordance coefficients in 204 male and female participants in a 12-month intervention using supplements to increase the intake of EPA and DHA. Concordance coefficients generally showed very poor agreement between AT FA and plasma NEFA at baseline SFA: 0·07; MUFA: 0·03; n-6 PUFA: 0·28; n-3 PUFA: 0·01). Participants were randomly divided into training (70 %) and validation (30 %) data sets, and models to predict AT and dietary FA were fitted using data from the training set, and their predictive ability was assessed using data from the validation set. AT n-6 PUFA and SFA were predicted from plasma NEFA with moderate accuracy (mean absolute percentage error n-6 PUFA: 11 % and SFA: 8 %), but predicted values were unable to distinguish between low, medium and high FA values, with only 25 % of n-6 PUFA and 33 % of SFA predicted values correctly assigned to the appropriate tertile group. Despite an association between AT and plasma NEFA EPA (P=0·001) and DHA (P=0·01) at baseline, there was no association after the intervention. To conclude, plasma NEFA are not a suitable surrogate for AT FA
nefa, adipose tissue fatty acids, eha supplementation, dha supplementation, prediction equations, surrogate markers
0007-1145
756-762
Walker, C.G.
612e7ef4-6bf9-42f6-9948-0ad00021fd49
Browning, L.M.
757097ae-415f-4f54-9e81-a129c5bcec83
Stecher, L.
f6c359b2-9e93-441c-a360-04f0e5355233
West, A.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Madden, J.
0771e352-d432-41ea-8a7e-4704c1efca46
Jebb, S.A.
c734720d-52eb-4656-a712-24398ef662f2
Calder, P.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Walker, C.G.
612e7ef4-6bf9-42f6-9948-0ad00021fd49
Browning, L.M.
757097ae-415f-4f54-9e81-a129c5bcec83
Stecher, L.
f6c359b2-9e93-441c-a360-04f0e5355233
West, A.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Madden, J.
0771e352-d432-41ea-8a7e-4704c1efca46
Jebb, S.A.
c734720d-52eb-4656-a712-24398ef662f2
Calder, P.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Walker, C.G., Browning, L.M., Stecher, L., West, A., Madden, J., Jebb, S.A. and Calder, P. (2015) Fatty acid profile of plasma NEFA does not reflect adipose tissue fatty acid profile. British Journal of Nutrition, 114 (5), 756-762. (doi:10.1017/S0007114515002251). (PMID:26205910)

Record type: Article

Abstract

Adipose tissue (AT) fatty acid (FA) composition partly reflects habitual dietary intake. Circulating NEFA are mobilised from AT and might act as a minimally invasive surrogate marker of AT FA profile. Agreement between twenty-eight FA in AT and plasma NEFA was assessed using concordance coefficients in 204 male and female participants in a 12-month intervention using supplements to increase the intake of EPA and DHA. Concordance coefficients generally showed very poor agreement between AT FA and plasma NEFA at baseline SFA: 0·07; MUFA: 0·03; n-6 PUFA: 0·28; n-3 PUFA: 0·01). Participants were randomly divided into training (70 %) and validation (30 %) data sets, and models to predict AT and dietary FA were fitted using data from the training set, and their predictive ability was assessed using data from the validation set. AT n-6 PUFA and SFA were predicted from plasma NEFA with moderate accuracy (mean absolute percentage error n-6 PUFA: 11 % and SFA: 8 %), but predicted values were unable to distinguish between low, medium and high FA values, with only 25 % of n-6 PUFA and 33 % of SFA predicted values correctly assigned to the appropriate tertile group. Despite an association between AT and plasma NEFA EPA (P=0·001) and DHA (P=0·01) at baseline, there was no association after the intervention. To conclude, plasma NEFA are not a suitable surrogate for AT FA

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Accepted/In Press date: 29 May 2015
Published date: September 2015
Keywords: nefa, adipose tissue fatty acids, eha supplementation, dha supplementation, prediction equations, surrogate markers
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 382261
URI: http://eprints.soton.ac.uk/id/eprint/382261
ISSN: 0007-1145
PURE UUID: 8536bbad-7de0-4a68-8e00-1f483179a73c
ORCID for P. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 22 Oct 2015 14:08
Last modified: 15 Mar 2024 02:50

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Contributors

Author: C.G. Walker
Author: L.M. Browning
Author: L. Stecher
Author: A. West
Author: J. Madden
Author: S.A. Jebb
Author: P. Calder ORCID iD

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