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Exposure to welding fumes and lower airway infection with Streptococcus pneumoniae

Exposure to welding fumes and lower airway infection with Streptococcus pneumoniae
Exposure to welding fumes and lower airway infection with Streptococcus pneumoniae
Background

Welders are at increased risk of pneumococcal pneumonia. The mechanism for this association is not known. The capacity of pneumococci to adhere to and infect lower airway cells is mediated by host-expressed platelet-activating factor receptor (PAFR).

Objective

We sought to assess the effect of mild steel welding fumes (MS-WF) on PAFR-dependent pneumococcal adhesion and infection to human airway cells in vitro and on pneumococcal airway infection in a mouse model.

Methods

The oxidative potential of MS-WF was assessed by their capacity to reduce antioxidants in vitro. Pneumococcal adhesion and infection of A549, BEAS-2B, and primary human bronchial airway cells were assessed by means of quantitative bacterial culture and expressed as colony-forming units (CFU). After intranasal instillation of MS-WF, mice were infected with Streptococcus pneumoniae, and bronchoalveolar lavage fluid (BALF) and lung CFU values were determined. PAFR protein levels were assessed by using immunofluorescence and immunohistochemistry, and PAFR mRNA expression was assessed by using quantitative PCR. PAFR was blocked by CV-3988, and oxidative stress was attenuated by N-acetylcysteine.

Results

MS-WF exhibited high oxidative potential. In A549 and BEAS-2B cells MS-WF increased pneumococcal adhesion and infection and PAFR protein expression. Both CV-3988 and N-acetylcysteine reduced MS-WF–stimulated pneumococcal adhesion and infection of airway cells. MS-WF increased mouse lung PAFR mRNA expression and increased BALF and lung pneumococcal CFU values. In MS-WF–exposed mice CV-3988 reduced BALF CFU values.

Conclusions

Hypersusceptibility of welders to pneumococcal pneumonia is in part mediated by the capacity of welding fumes to increase PAFR-dependent pneumococcal adhesion and infection of lower airway cells.
occupational disease, welding fumes, platelet-activating factor receptor, streptococcus pneumonia, pneumonia, bacterial adhesion and infection
0091-6749
1-15
Suri, Reetika
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Periselneris, Jimstan
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Lanone, Sophie
7ef9c528-e91b-4d1b-80e7-dde9216fb05c
Zeidler-Erdely, Patti C.
08b83073-983a-4997-af3d-0cdff96e8421
Melton, Geoffrey
7d1080b8-b926-4b8c-99b3-89eb30c31d71
Palmer, Keith T.
0cfe63f0-1d33-40ff-ae8c-6c33601df850
Andujar, Pascal
d9894d5e-fc2d-4ebd-9727-2ad4fd9db302
Antonini, James M.
61188c4a-ff1a-42ab-a416-abd3e979deb9
Cohignac, Vanessa
dc784747-efac-4e60-be44-031b97f2c592
Erdely, Aaron
45aa92da-bf53-4d8b-a322-a31c799db555
Jose, Ricardo J.
730be71e-7845-4b72-b2a9-46e4d4213034
Mudway, Ian
2921e747-b61a-4979-95a0-f08b2527474a
Brown, Jeremy
19418121-0e51-483c-9eb5-6c0f711f5f75
Grigg, Jonathan
587e2313-7a36-4d61-8010-45447565cfcd
Suri, Reetika
32d2b357-5e64-4ace-9b48-e226e8f91206
Periselneris, Jimstan
76e7d509-817c-4c6a-b913-2efb048159fd
Lanone, Sophie
7ef9c528-e91b-4d1b-80e7-dde9216fb05c
Zeidler-Erdely, Patti C.
08b83073-983a-4997-af3d-0cdff96e8421
Melton, Geoffrey
7d1080b8-b926-4b8c-99b3-89eb30c31d71
Palmer, Keith T.
0cfe63f0-1d33-40ff-ae8c-6c33601df850
Andujar, Pascal
d9894d5e-fc2d-4ebd-9727-2ad4fd9db302
Antonini, James M.
61188c4a-ff1a-42ab-a416-abd3e979deb9
Cohignac, Vanessa
dc784747-efac-4e60-be44-031b97f2c592
Erdely, Aaron
45aa92da-bf53-4d8b-a322-a31c799db555
Jose, Ricardo J.
730be71e-7845-4b72-b2a9-46e4d4213034
Mudway, Ian
2921e747-b61a-4979-95a0-f08b2527474a
Brown, Jeremy
19418121-0e51-483c-9eb5-6c0f711f5f75
Grigg, Jonathan
587e2313-7a36-4d61-8010-45447565cfcd

Suri, Reetika, Periselneris, Jimstan, Lanone, Sophie, Zeidler-Erdely, Patti C., Melton, Geoffrey, Palmer, Keith T., Andujar, Pascal, Antonini, James M., Cohignac, Vanessa, Erdely, Aaron, Jose, Ricardo J., Mudway, Ian, Brown, Jeremy and Grigg, Jonathan (2015) Exposure to welding fumes and lower airway infection with Streptococcus pneumoniae. Journal of Allergy and Clinical Immunology, 1-15. (doi:10.1016/j.jaci.2015.06.033). (PMID:26277596)

Record type: Article

Abstract

Background

Welders are at increased risk of pneumococcal pneumonia. The mechanism for this association is not known. The capacity of pneumococci to adhere to and infect lower airway cells is mediated by host-expressed platelet-activating factor receptor (PAFR).

Objective

We sought to assess the effect of mild steel welding fumes (MS-WF) on PAFR-dependent pneumococcal adhesion and infection to human airway cells in vitro and on pneumococcal airway infection in a mouse model.

Methods

The oxidative potential of MS-WF was assessed by their capacity to reduce antioxidants in vitro. Pneumococcal adhesion and infection of A549, BEAS-2B, and primary human bronchial airway cells were assessed by means of quantitative bacterial culture and expressed as colony-forming units (CFU). After intranasal instillation of MS-WF, mice were infected with Streptococcus pneumoniae, and bronchoalveolar lavage fluid (BALF) and lung CFU values were determined. PAFR protein levels were assessed by using immunofluorescence and immunohistochemistry, and PAFR mRNA expression was assessed by using quantitative PCR. PAFR was blocked by CV-3988, and oxidative stress was attenuated by N-acetylcysteine.

Results

MS-WF exhibited high oxidative potential. In A549 and BEAS-2B cells MS-WF increased pneumococcal adhesion and infection and PAFR protein expression. Both CV-3988 and N-acetylcysteine reduced MS-WF–stimulated pneumococcal adhesion and infection of airway cells. MS-WF increased mouse lung PAFR mRNA expression and increased BALF and lung pneumococcal CFU values. In MS-WF–exposed mice CV-3988 reduced BALF CFU values.

Conclusions

Hypersusceptibility of welders to pneumococcal pneumonia is in part mediated by the capacity of welding fumes to increase PAFR-dependent pneumococcal adhesion and infection of lower airway cells.

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More information

Accepted/In Press date: 29 June 2015
e-pub ahead of print date: 12 August 2015
Keywords: occupational disease, welding fumes, platelet-activating factor receptor, streptococcus pneumonia, pneumonia, bacterial adhesion and infection
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 382457
URI: http://eprints.soton.ac.uk/id/eprint/382457
ISSN: 0091-6749
PURE UUID: e7526332-9f36-4e8f-b699-a5bd156ed834

Catalogue record

Date deposited: 05 Oct 2015 11:27
Last modified: 14 Mar 2024 21:28

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Contributors

Author: Reetika Suri
Author: Jimstan Periselneris
Author: Sophie Lanone
Author: Patti C. Zeidler-Erdely
Author: Geoffrey Melton
Author: Keith T. Palmer
Author: Pascal Andujar
Author: James M. Antonini
Author: Vanessa Cohignac
Author: Aaron Erdely
Author: Ricardo J. Jose
Author: Ian Mudway
Author: Jeremy Brown
Author: Jonathan Grigg

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