The University of Southampton
University of Southampton Institutional Repository

Measurements of nitrogen fixation in the oligotrophic North Pacific Subtropical Gyre using a free-drifting submersible incubation device

Measurements of nitrogen fixation in the oligotrophic North Pacific Subtropical Gyre using a free-drifting submersible incubation device
Measurements of nitrogen fixation in the oligotrophic North Pacific Subtropical Gyre using a free-drifting submersible incubation device
One challenge in field-based marine microbial ecology is to achieve sufficient spatial resolution to obtain representative information about microbial distributions and biogeochemical processes. The challenges are exacerbated when conducting rate measurements of biological processes due to potential perturbations during sampling and incubation. Here we present the first application of a robotic microlaboratory, the 4 L-submersible incubation device (SID), for conducting in situ measurements of the rates of biological nitrogen (N2) fixation (BNF). The free-drifting autonomous instrument obtains samples from the water column that are incubated in situ after the addition of 15N2 tracer. After each of up to four consecutive incubation experiments, the 4-L sample is filtered and chemically preserved. Measured BNF rates from two deployments of the SID in the oligotrophic North Pacific ranged from 0.8 to 2.8 nmol N L?1 day?1, values comparable with simultaneous rate measurements obtained using traditional conductivity–temperature–depth (CTD)–rosette sampling followed by on-deck or in situ incubation. Future deployments of the SID will help to better resolve spatial variability of oceanic BNF, particularly in areas where recovery of seawater samples by CTD compromises their integrity, e.g. anoxic habitats.
nitrogen fixation, in situ device, Pacific Ocean, diazotroph
0142-7873
727-739
Bombar, Deniz
38485332-c4a9-4e8a-962d-6730b562dd9b
Taylor, Craig D.
93d2a3f9-9649-4ed5-84fb-184c9d0f4d22
Wilson, Samuel T.
b5b35cff-7d54-4df2-bfef-9cb9dd1114b8
Robidart, Julie C.
1d72ac92-7dca-49a0-9f9e-26798387e66b
Rabines, Ariel
f0caf470-6b79-46ff-86c6-7e9ad18894e7
Turk-Kubo, Kendra A.
25eb3e4c-7080-4841-b795-28ac70efbdb9
Kemp, John N.
cda0a45c-f718-433b-aad6-76867ba08d04
Karl, David M.
c462949a-fd21-40c5-968a-61d5ec967858
Zehr, Jonathan P.
df07e726-0814-4247-8a74-942c07bd23e2
Bombar, Deniz
38485332-c4a9-4e8a-962d-6730b562dd9b
Taylor, Craig D.
93d2a3f9-9649-4ed5-84fb-184c9d0f4d22
Wilson, Samuel T.
b5b35cff-7d54-4df2-bfef-9cb9dd1114b8
Robidart, Julie C.
1d72ac92-7dca-49a0-9f9e-26798387e66b
Rabines, Ariel
f0caf470-6b79-46ff-86c6-7e9ad18894e7
Turk-Kubo, Kendra A.
25eb3e4c-7080-4841-b795-28ac70efbdb9
Kemp, John N.
cda0a45c-f718-433b-aad6-76867ba08d04
Karl, David M.
c462949a-fd21-40c5-968a-61d5ec967858
Zehr, Jonathan P.
df07e726-0814-4247-8a74-942c07bd23e2

Bombar, Deniz, Taylor, Craig D., Wilson, Samuel T., Robidart, Julie C., Rabines, Ariel, Turk-Kubo, Kendra A., Kemp, John N., Karl, David M. and Zehr, Jonathan P. (2015) Measurements of nitrogen fixation in the oligotrophic North Pacific Subtropical Gyre using a free-drifting submersible incubation device. Journal of Plankton Research, 37 (4), 727-739. (doi:10.1093/plankt/fbv049).

Record type: Article

Abstract

One challenge in field-based marine microbial ecology is to achieve sufficient spatial resolution to obtain representative information about microbial distributions and biogeochemical processes. The challenges are exacerbated when conducting rate measurements of biological processes due to potential perturbations during sampling and incubation. Here we present the first application of a robotic microlaboratory, the 4 L-submersible incubation device (SID), for conducting in situ measurements of the rates of biological nitrogen (N2) fixation (BNF). The free-drifting autonomous instrument obtains samples from the water column that are incubated in situ after the addition of 15N2 tracer. After each of up to four consecutive incubation experiments, the 4-L sample is filtered and chemically preserved. Measured BNF rates from two deployments of the SID in the oligotrophic North Pacific ranged from 0.8 to 2.8 nmol N L?1 day?1, values comparable with simultaneous rate measurements obtained using traditional conductivity–temperature–depth (CTD)–rosette sampling followed by on-deck or in situ incubation. Future deployments of the SID will help to better resolve spatial variability of oceanic BNF, particularly in areas where recovery of seawater samples by CTD compromises their integrity, e.g. anoxic habitats.

Full text not available from this repository.

More information

Published date: July 2015
Keywords: nitrogen fixation, in situ device, Pacific Ocean, diazotroph
Organisations: Ocean Technology and Engineering

Identifiers

Local EPrints ID: 382534
URI: http://eprints.soton.ac.uk/id/eprint/382534
ISSN: 0142-7873
PURE UUID: 95a6cd0e-bfc6-4d51-b56e-d158d1cc5ba9

Catalogue record

Date deposited: 06 Oct 2015 15:19
Last modified: 15 Jul 2019 21:02

Export record

Altmetrics

Contributors

Author: Deniz Bombar
Author: Craig D. Taylor
Author: Samuel T. Wilson
Author: Julie C. Robidart
Author: Ariel Rabines
Author: Kendra A. Turk-Kubo
Author: John N. Kemp
Author: David M. Karl
Author: Jonathan P. Zehr

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×