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Maternal high-fat diet and offspring expression levels of vitamin K-dependent proteins

Maternal high-fat diet and offspring expression levels of vitamin K-dependent proteins
Maternal high-fat diet and offspring expression levels of vitamin K-dependent proteins
Studies suggest that bone growth and development and susceptibility to vascular disease in later life are influenced by maternal nutrition during intrauterine and early postnatal life. There is evidence for a role of vitamin K-dependent proteins (VKDPs) including osteocalcin, matrix Gla protein, periostin, and growth-arrest specific- protein 6, in both bone and vascular development. We have examined whether there are alterations in these VKDPs in bone and vascular tissue from offspring of mothers subjected to a nutritional challenge: a high-fat diet during pregnancy and postnatally, using 6-week-old mouse offspring. Bone site-specific and sex-specific differences across femoral and vertebral bone in male and female offspring were observed. Overall a high-fat maternal diet and offspring diet exacerbated the bone changes observed. Sex-specific differences and tissue-specific differences were observed in VKDP levels in aorta tissue from high-fat diet-fed female offspring from high-fat diet-fed mothers displaying increased levels of Gas6 and Ggcx compared with those of female controls. In contrast, differences were seen in VKDP levels in femoral bone of female offspring with lower expression levels of Mgp in offspring of mothers fed a high-fat diet compared with those of controls. We observed a significant correlation in Mgp expression levels within the femur to measures of bone structure of the femur and vertebra, particularly in the male offspring cohort. In summary, the current study has highlighted the importance of maternal nutrition on offspring bone development and the correlation of VKDPs to bone structure.
0013-7227
4749-4761
Lanham, S.A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Cagampang, F.R.
7cf57d52-4a65-4554-8306-ed65226bc50e
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Lanham, S.A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Cagampang, F.R.
7cf57d52-4a65-4554-8306-ed65226bc50e
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Lanham, S.A., Cagampang, F.R. and Oreffo, R.O.C. (2014) Maternal high-fat diet and offspring expression levels of vitamin K-dependent proteins. Endocrinology, 155 (12), 4749-4761. (doi:10.1210/en.2014-1188). (PMID:25279792)

Record type: Article

Abstract

Studies suggest that bone growth and development and susceptibility to vascular disease in later life are influenced by maternal nutrition during intrauterine and early postnatal life. There is evidence for a role of vitamin K-dependent proteins (VKDPs) including osteocalcin, matrix Gla protein, periostin, and growth-arrest specific- protein 6, in both bone and vascular development. We have examined whether there are alterations in these VKDPs in bone and vascular tissue from offspring of mothers subjected to a nutritional challenge: a high-fat diet during pregnancy and postnatally, using 6-week-old mouse offspring. Bone site-specific and sex-specific differences across femoral and vertebral bone in male and female offspring were observed. Overall a high-fat maternal diet and offspring diet exacerbated the bone changes observed. Sex-specific differences and tissue-specific differences were observed in VKDP levels in aorta tissue from high-fat diet-fed female offspring from high-fat diet-fed mothers displaying increased levels of Gas6 and Ggcx compared with those of female controls. In contrast, differences were seen in VKDP levels in femoral bone of female offspring with lower expression levels of Mgp in offspring of mothers fed a high-fat diet compared with those of controls. We observed a significant correlation in Mgp expression levels within the femur to measures of bone structure of the femur and vertebra, particularly in the male offspring cohort. In summary, the current study has highlighted the importance of maternal nutrition on offspring bone development and the correlation of VKDPs to bone structure.

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Accepted/In Press date: 26 September 2014
e-pub ahead of print date: 3 October 2014
Published date: December 2014
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 382859
URI: http://eprints.soton.ac.uk/id/eprint/382859
ISSN: 0013-7227
PURE UUID: 9a973dc5-3b17-400d-a960-0e94b0d07330
ORCID for S.A. Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for F.R. Cagampang: ORCID iD orcid.org/0000-0003-4404-9853
ORCID for R.O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 03 Nov 2015 15:34
Last modified: 15 Mar 2024 03:14

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Contributors

Author: S.A. Lanham ORCID iD
Author: F.R. Cagampang ORCID iD
Author: R.O.C. Oreffo ORCID iD

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