Development of peptide nucleic acid probes to detect Helicobacter pylori in diverse species potable water biofilms
Development of peptide nucleic acid probes to detect Helicobacter pylori in diverse species potable water biofilms
The application of novel technology peptide nucleic acid (PNA) molecular probes for the visualization of Helicobacter pylori in biofilms has been assessed. The probes selected were highly specific for the pathogen and did not hybridize with any of the autochthonous bacteria isolated or with any other component from the water biofilm. The use of this technique made the pathogen detection quick and reliable however, the presence of autofluorescent microorganisms and stacks in the biofilm samples implied that care had to be taken not to misidentify the bacteria. H. pylori can successfully incorporate within biofilms and all three different types of morphology were identified. PNA probes appear to be a promising new technique for the in situ visualization of microorganisms in biofilms and a further development of this technique may allow the simultaneous detection of different species.
0-9545756-0-1
105-112
Azevedo, Nuno
0ae641ab-d4ea-4fcd-b17b-b28bd01a7593
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577
Keevil, Charles
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
1 July 2003
Azevedo, Nuno
0ae641ab-d4ea-4fcd-b17b-b28bd01a7593
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577
Keevil, Charles
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Azevedo, Nuno, Vieira, M.J. and Keevil, Charles
(2003)
Development of peptide nucleic acid probes to detect Helicobacter pylori in diverse species potable water biofilms.
Walker, Jimmy, McBain, Andrew, Allison, David, Brading, Melanie, Rikard, Alexander and Verran, Joanna
(eds.)
In Biofilm Communities: Order From Chaos?
BioLine.
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Record type:
Conference or Workshop Item
(Paper)
Abstract
The application of novel technology peptide nucleic acid (PNA) molecular probes for the visualization of Helicobacter pylori in biofilms has been assessed. The probes selected were highly specific for the pathogen and did not hybridize with any of the autochthonous bacteria isolated or with any other component from the water biofilm. The use of this technique made the pathogen detection quick and reliable however, the presence of autofluorescent microorganisms and stacks in the biofilm samples implied that care had to be taken not to misidentify the bacteria. H. pylori can successfully incorporate within biofilms and all three different types of morphology were identified. PNA probes appear to be a promising new technique for the in situ visualization of microorganisms in biofilms and a further development of this technique may allow the simultaneous detection of different species.
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More information
Published date: 1 July 2003
Venue - Dates:
Sixth Meeting of the Biofilm Club, Powys, United Kingdom, 2003-06-30
Organisations:
Environmental
Identifiers
Local EPrints ID: 383162
URI: http://eprints.soton.ac.uk/id/eprint/383162
ISBN: 0-9545756-0-1
PURE UUID: 75ae3aa3-2fc3-4b35-b4f6-9a41e39db03e
Catalogue record
Date deposited: 11 Nov 2015 15:47
Last modified: 12 Dec 2021 03:17
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Contributors
Author:
Nuno Azevedo
Author:
M.J. Vieira
Editor:
Jimmy Walker
Editor:
Andrew McBain
Editor:
David Allison
Editor:
Melanie Brading
Editor:
Alexander Rikard
Editor:
Joanna Verran
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