Proteomic analysis of the human skin proteome after in vivo treatment with sodium dodecyl sulphate
Proteomic analysis of the human skin proteome after in vivo treatment with sodium dodecyl sulphate
BACKGROUND: Skin has a variety of functions that are incompletely understood at the molecular level. As the most accessible tissue in the body it often reveals the first signs of inflammation or infection and also represents a potentially valuable source of biomarkers for several diseases. In this study we surveyed the skin proteome qualitatively using gel electrophoresis, liquid chromatography tandem mass spectrometry (GeLC-MS/MS) and quantitatively using an isobaric tagging strategy (iTRAQ) to characterise the response of human skin following exposure to sodium dodecyl sulphate (SDS).
RESULTS: A total of 653 skin proteins were assigned, 159 of which were identified using GeLC-MS/MS and 616 using iTRAQ, representing the most comprehensive proteomic study in human skin tissue. Statistical analysis of the available iTRAQ data did not reveal any significant differences in the measured skin proteome after 4 hours exposure to the model irritant SDS.
CONCLUSIONS: This study represents the first step in defining the critical response to an irritant at the level of the proteome and provides a valuable resource for further studies at the later stages of irritant exposure.
e97772-[10pp]
Parkinson, Erika
b7294dcc-43d3-46c4-bd19-7f6795b80fe6
Skipp, P.
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Aleksic, Maja
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Garrow, Andrew
8d24d000-5765-45f7-88be-c848fc1568f5
Dadd, Tony
b38a8e6a-8ac1-43ac-a165-d677f8ec94fe
Hughes, Michael
d27c81ff-ee2c-4c40-a663-0f98618cf960
Clough, G
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O'Connor, C.D.
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21 May 2014
Parkinson, Erika
b7294dcc-43d3-46c4-bd19-7f6795b80fe6
Skipp, P.
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
Aleksic, Maja
152a8f09-84c2-4ebb-94c7-79c31edf53aa
Garrow, Andrew
8d24d000-5765-45f7-88be-c848fc1568f5
Dadd, Tony
b38a8e6a-8ac1-43ac-a165-d677f8ec94fe
Hughes, Michael
d27c81ff-ee2c-4c40-a663-0f98618cf960
Clough, G
9f19639e-a929-4976-ac35-259f9011c494
O'Connor, C.D.
c1f6776c-2303-499b-a788-2c4c39a0e5af
Parkinson, Erika, Skipp, P., Aleksic, Maja, Garrow, Andrew, Dadd, Tony, Hughes, Michael, Clough, G and O'Connor, C.D.
(2014)
Proteomic analysis of the human skin proteome after in vivo treatment with sodium dodecyl sulphate.
PLoS ONE, 9 (5), .
(doi:10.1371/journal.pone.0097772).
(PMID:24849295)
Abstract
BACKGROUND: Skin has a variety of functions that are incompletely understood at the molecular level. As the most accessible tissue in the body it often reveals the first signs of inflammation or infection and also represents a potentially valuable source of biomarkers for several diseases. In this study we surveyed the skin proteome qualitatively using gel electrophoresis, liquid chromatography tandem mass spectrometry (GeLC-MS/MS) and quantitatively using an isobaric tagging strategy (iTRAQ) to characterise the response of human skin following exposure to sodium dodecyl sulphate (SDS).
RESULTS: A total of 653 skin proteins were assigned, 159 of which were identified using GeLC-MS/MS and 616 using iTRAQ, representing the most comprehensive proteomic study in human skin tissue. Statistical analysis of the available iTRAQ data did not reveal any significant differences in the measured skin proteome after 4 hours exposure to the model irritant SDS.
CONCLUSIONS: This study represents the first step in defining the critical response to an irritant at the level of the proteome and provides a valuable resource for further studies at the later stages of irritant exposure.
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Accepted/In Press date: 24 April 2014
Published date: 21 May 2014
Organisations:
Faculty of Medicine
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Local EPrints ID: 383567
URI: http://eprints.soton.ac.uk/id/eprint/383567
ISSN: 1932-6203
PURE UUID: 00b3691c-8237-4f13-b14e-22f285150f7f
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Date deposited: 24 Nov 2015 10:04
Last modified: 15 Mar 2024 02:53
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Author:
Maja Aleksic
Author:
Andrew Garrow
Author:
Tony Dadd
Author:
Michael Hughes
Author:
C.D. O'Connor
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