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Inhibition of tumour necrosis factor-alpha and interleukin 6 production by mononuclear cells following dietary fish-oil supplementation in healthy men and response to antioxidant co-supplementation.

Inhibition of tumour necrosis factor-alpha and interleukin 6 production by mononuclear cells following dietary fish-oil supplementation in healthy men and response to antioxidant co-supplementation.
Inhibition of tumour necrosis factor-alpha and interleukin 6 production by mononuclear cells following dietary fish-oil supplementation in healthy men and response to antioxidant co-supplementation.
Increased dietary consumption of the n-3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (20 : 5n-3; EPA) and docosahexaenoic acid (22 : 6n-6; DHA) is associated with their incorporation into circulating phospholipid and increased production of lipid peroxide metabolites. The relationship between peripheral blood mononuclear cell (PBMC) function, n-3 PUFA intake and antioxidant co-supplementation is poorly defined. We therefore investigated tumour necrosis factor (TNF)-alpha and interleukin (IL) 6 production by PBMC and phospholipid fatty acid composition in plasma and erythrocytes of healthy male subjects (n 16) receiving supplemental intakes of 0.3, 1.0 and 2.0 g EPA+DHA/d, as consecutive 4-week courses. All subjects were randomised in a double-blind manner to receive a concurrent antioxidant supplement (200 microg Se, 3 mg Mn, 30 mg D-alpha-tocopheryl succinate, 90 mg ascorbic acid, 450 microg vitamin A (beta-carotene and retinol)) or placebo. There was a positive dose-dependent relationship between dietary n-3 PUFA intake and EPA and DHA incorporation into plasma phosphatidylcholine and erythrocyte phosphatidylethanolamine, with a tendency towards a plateau at higher levels of intake. Production of TNF-alpha and IL-6 by PBMC decreased with increasing n-3 PUFA intake but tended towards a 'U-shaped' dose response. Both responses appeared to be augmented by antioxidant co-supplementation at intermediate supplementary n-3 PUFA intakes. Thus, increased dietary n-3 PUFA consumption resulted in defined but contrasting patterns of modulation of phospholipid fatty acid composition and PBMC function, which were further influenced by antioxidant intake.
fish oil, antioxidants, tumour necrosis factor, interleukin 6
0007-1145
405-412
Trebble, T.
14b25cc8-5a3f-4aef-aa93-a29e97241f7e
Arden, N.K.
23af958d-835c-4d79-be54-4bbe4c68077f
Stroud, M.A.
1665ae65-0898-4848-bf0d-baec8f2bb078
Wootton, S.A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Burdge, G.C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Miles, E.A.
20332899-ecdb-4214-95bc-922dde36d416
Ballinger, A.B.
08cc976f-3c4d-44df-b1bf-eb46172101d8
Thompson, R.L.
1a394a6d-b006-4aec-b9be-b3e6c16fdb7b
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Trebble, T.
14b25cc8-5a3f-4aef-aa93-a29e97241f7e
Arden, N.K.
23af958d-835c-4d79-be54-4bbe4c68077f
Stroud, M.A.
1665ae65-0898-4848-bf0d-baec8f2bb078
Wootton, S.A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Burdge, G.C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Miles, E.A.
20332899-ecdb-4214-95bc-922dde36d416
Ballinger, A.B.
08cc976f-3c4d-44df-b1bf-eb46172101d8
Thompson, R.L.
1a394a6d-b006-4aec-b9be-b3e6c16fdb7b
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Trebble, T., Arden, N.K., Stroud, M.A., Wootton, S.A., Burdge, G.C., Miles, E.A., Ballinger, A.B., Thompson, R.L. and Calder, P.C. (2003) Inhibition of tumour necrosis factor-alpha and interleukin 6 production by mononuclear cells following dietary fish-oil supplementation in healthy men and response to antioxidant co-supplementation. British Journal of Nutrition, 90 (2), 405-412. (doi:10.1079/BJN2003892). (PMID:12908901)

Record type: Article

Abstract

Increased dietary consumption of the n-3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (20 : 5n-3; EPA) and docosahexaenoic acid (22 : 6n-6; DHA) is associated with their incorporation into circulating phospholipid and increased production of lipid peroxide metabolites. The relationship between peripheral blood mononuclear cell (PBMC) function, n-3 PUFA intake and antioxidant co-supplementation is poorly defined. We therefore investigated tumour necrosis factor (TNF)-alpha and interleukin (IL) 6 production by PBMC and phospholipid fatty acid composition in plasma and erythrocytes of healthy male subjects (n 16) receiving supplemental intakes of 0.3, 1.0 and 2.0 g EPA+DHA/d, as consecutive 4-week courses. All subjects were randomised in a double-blind manner to receive a concurrent antioxidant supplement (200 microg Se, 3 mg Mn, 30 mg D-alpha-tocopheryl succinate, 90 mg ascorbic acid, 450 microg vitamin A (beta-carotene and retinol)) or placebo. There was a positive dose-dependent relationship between dietary n-3 PUFA intake and EPA and DHA incorporation into plasma phosphatidylcholine and erythrocyte phosphatidylethanolamine, with a tendency towards a plateau at higher levels of intake. Production of TNF-alpha and IL-6 by PBMC decreased with increasing n-3 PUFA intake but tended towards a 'U-shaped' dose response. Both responses appeared to be augmented by antioxidant co-supplementation at intermediate supplementary n-3 PUFA intakes. Thus, increased dietary n-3 PUFA consumption resulted in defined but contrasting patterns of modulation of phospholipid fatty acid composition and PBMC function, which were further influenced by antioxidant intake.

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Published date: August 2003
Keywords: fish oil, antioxidants, tumour necrosis factor, interleukin 6
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 383823
URI: http://eprints.soton.ac.uk/id/eprint/383823
ISSN: 0007-1145
PURE UUID: d9076ec5-a80a-4517-ac2f-d98f936b868a
ORCID for G.C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967
ORCID for E.A. Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for P.C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 26 Nov 2015 11:34
Last modified: 15 Mar 2024 02:50

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Contributors

Author: T. Trebble
Author: N.K. Arden
Author: M.A. Stroud
Author: S.A. Wootton
Author: G.C. Burdge ORCID iD
Author: E.A. Miles ORCID iD
Author: A.B. Ballinger
Author: R.L. Thompson
Author: P.C. Calder ORCID iD

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