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Stable-isotope method for determining the gastrointestinal handling of [1-13C]palmitic acid

Stable-isotope method for determining the gastrointestinal handling of [1-13C]palmitic acid
Stable-isotope method for determining the gastrointestinal handling of [1-13C]palmitic acid
The 13C enrichment in individual fatty acids extracted from human feces following the oral administration of [1-13C]palmitic acid has been determined using a novel approach based upon gas chromatography-isotope ratio mass spectrometry. The method was established and tested for precision and repeatability. Analytical precision was determined from 10 repeated injections of a sample containing 16:0 and 18:0 with levels of delta 13C abundance measured at -34.01 +/- 0.60 and -23.62 +/- 0.95 delta per mil (parts per thousand) (/1000), respectively (mean +/- SD). For the repeatability study, measurement of enrichment of the same mixture of unlabeled fatty acid methyl ester (FAME) standards (13:0, 14:0, 16:0, and 18:0) was found to have standard deviations (0.45, 0.56, 1.46 and 1.54/1000, respectively). When labeled [1-13C]palmitic acid was serially diluted with naturally enriched palmitic acid, a linear relationship was obtained to a dilution of 10% enriched compound (530/1000). FAME were prepared from two fecal samples from a normal healthy adult; the first, a baseline specimen, containing no added label and the second, followed a single oral dose of [1-13C]palmitic acid and was enriched. Enrichment in 13C was confined to the solvent-soluble fraction following lipid extraction, and was only identified with prior acidification. The enrichments were measured in triplicate, baseline sample -32.66 +/- 0.5/1000, enriched sample +268.61 +/- 8.0/1000. Enrichment was restricted to the labeled species consumed, 16:0. The methodology described here allows for the separation of compounds prior to the determination of enrichment and can be utilized to contribute to a more complete description of the gastrointestinal handling of labeled substrates than previously obtained.
0024-4201
337-340
Stolinski, M.
56fb652b-6b99-4aac-adf4-01f49a03b080
Murphy, J.L.
99e7863d-f870-438a-b4f5-bec8a2e78ebd
Jones, A.E.
61f65ad1-ae5c-47f6-b159-63aa6a5178b5
Jackson, A.A.
c9a12d7c-b4d6-4c92-820e-890a688379ef
Wootton, S.A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Stolinski, M.
56fb652b-6b99-4aac-adf4-01f49a03b080
Murphy, J.L.
99e7863d-f870-438a-b4f5-bec8a2e78ebd
Jones, A.E.
61f65ad1-ae5c-47f6-b159-63aa6a5178b5
Jackson, A.A.
c9a12d7c-b4d6-4c92-820e-890a688379ef
Wootton, S.A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c

Stolinski, M., Murphy, J.L., Jones, A.E., Jackson, A.A. and Wootton, S.A. (1997) Stable-isotope method for determining the gastrointestinal handling of [1-13C]palmitic acid. Lipids, 32 (3), 337-340. (PMID:9076672)

Record type: Article

Abstract

The 13C enrichment in individual fatty acids extracted from human feces following the oral administration of [1-13C]palmitic acid has been determined using a novel approach based upon gas chromatography-isotope ratio mass spectrometry. The method was established and tested for precision and repeatability. Analytical precision was determined from 10 repeated injections of a sample containing 16:0 and 18:0 with levels of delta 13C abundance measured at -34.01 +/- 0.60 and -23.62 +/- 0.95 delta per mil (parts per thousand) (/1000), respectively (mean +/- SD). For the repeatability study, measurement of enrichment of the same mixture of unlabeled fatty acid methyl ester (FAME) standards (13:0, 14:0, 16:0, and 18:0) was found to have standard deviations (0.45, 0.56, 1.46 and 1.54/1000, respectively). When labeled [1-13C]palmitic acid was serially diluted with naturally enriched palmitic acid, a linear relationship was obtained to a dilution of 10% enriched compound (530/1000). FAME were prepared from two fecal samples from a normal healthy adult; the first, a baseline specimen, containing no added label and the second, followed a single oral dose of [1-13C]palmitic acid and was enriched. Enrichment in 13C was confined to the solvent-soluble fraction following lipid extraction, and was only identified with prior acidification. The enrichments were measured in triplicate, baseline sample -32.66 +/- 0.5/1000, enriched sample +268.61 +/- 8.0/1000. Enrichment was restricted to the labeled species consumed, 16:0. The methodology described here allows for the separation of compounds prior to the determination of enrichment and can be utilized to contribute to a more complete description of the gastrointestinal handling of labeled substrates than previously obtained.

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More information

Published date: March 1997
Organisations: Human Development & Health

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Local EPrints ID: 383849
URI: http://eprints.soton.ac.uk/id/eprint/383849
ISSN: 0024-4201
PURE UUID: 1fc4b7d4-1d23-45f9-a0bb-e470583a4ab8

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Date deposited: 26 Nov 2015 12:25
Last modified: 22 Jul 2022 19:38

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Contributors

Author: M. Stolinski
Author: J.L. Murphy
Author: A.E. Jones
Author: A.A. Jackson
Author: S.A. Wootton

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