The University of Southampton
University of Southampton Institutional Repository

Effects of controlled diesel exhaust exposure on apoptosis and proliferation markers in bronchial epithelium – an in vivo bronchoscopy study on asthmatics, rhinitics and healthy subjects

Effects of controlled diesel exhaust exposure on apoptosis and proliferation markers in bronchial epithelium – an in vivo bronchoscopy study on asthmatics, rhinitics and healthy subjects
Effects of controlled diesel exhaust exposure on apoptosis and proliferation markers in bronchial epithelium – an in vivo bronchoscopy study on asthmatics, rhinitics and healthy subjects
Background

Epidemiological evidence demonstrates that exposure to traffic-derived pollution worsens respiratory symptoms in asthmatics, but controlled human exposure studies have failed to provide a mechanism for this effect. Here we investigated whether diesel exhaust (DE) would induce apoptosis or proliferation in the bronchial epithelium in vivo and thus contribute to respiratory symptoms.

Methods

Moderate (n?=?16) and mild (n?=?16) asthmatics, atopic non-asthmatic controls (rhinitics) (n?=?13) and healthy controls (n?=?21) were exposed to filtered air or DE (100 ?g/m 3 ) for 2 h, on two separate occasions. Bronchial biopsies were taken 18 h post-exposure and immunohistochemically analysed for pro-apoptotic and anti-apoptotic proteins (Bad, Bak, p85 PARP, Fas, Bcl-2) and a marker of proliferation (Ki67). Positive staining was assessed within the epithelium using computerized image analysis.

Results

No evidence of epithelial apoptosis or proliferation was observed in healthy, allergic or asthmatic airways following DE challenge.

Conclusion

In the present study, we investigated whether DE exposure would affect markers of proliferation and apoptosis in the bronchial epithelium of asthmatics, rhinitics and healthy controls, providing a mechanistic basis for the reported increased airway sensitivity in asthmatics to air pollutants. In this first in vivo exposure investigation, we found no evidence of diesel exhaust-induced effects on these processes in the subject groups investigated.
1471-2466
1-9
Behndig, Annelie F.
8b7190cd-e3ed-42a0-bc5c-8ae83c402097
Shanmuganathan, Karthika
c3fb27c1-2f48-4ccb-879b-918c512ca0c2
Whitmarsh, Laura
da550151-2aa4-453f-9a3c-34cac4777610
Stenfors, Nikolai
69c8966c-e8f8-40f8-b8b2-8e4f918953f0
Brown, Joanna L.
68251ae9-5625-4980-b752-38c241406ca8
Frew, Anthony J.
4887b766-67c6-4d69-940d-4c06c0890b76
Kelly, Frank J.
8eda554f-c23c-4321-b5e2-b99a72dfd0aa
Mudway, Ian S.
a9f9b1a8-2a5b-4b1f-836d-8649d3621522
Sandström, Thomas
77a4c11d-a84f-4a52-9bfe-4ec83f62363a
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Behndig, Annelie F.
8b7190cd-e3ed-42a0-bc5c-8ae83c402097
Shanmuganathan, Karthika
c3fb27c1-2f48-4ccb-879b-918c512ca0c2
Whitmarsh, Laura
da550151-2aa4-453f-9a3c-34cac4777610
Stenfors, Nikolai
69c8966c-e8f8-40f8-b8b2-8e4f918953f0
Brown, Joanna L.
68251ae9-5625-4980-b752-38c241406ca8
Frew, Anthony J.
4887b766-67c6-4d69-940d-4c06c0890b76
Kelly, Frank J.
8eda554f-c23c-4321-b5e2-b99a72dfd0aa
Mudway, Ian S.
a9f9b1a8-2a5b-4b1f-836d-8649d3621522
Sandström, Thomas
77a4c11d-a84f-4a52-9bfe-4ec83f62363a
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8

Behndig, Annelie F., Shanmuganathan, Karthika, Whitmarsh, Laura, Stenfors, Nikolai, Brown, Joanna L., Frew, Anthony J., Kelly, Frank J., Mudway, Ian S., Sandström, Thomas and Wilson, Susan J. (2015) Effects of controlled diesel exhaust exposure on apoptosis and proliferation markers in bronchial epithelium – an in vivo bronchoscopy study on asthmatics, rhinitics and healthy subjects. BMC Pulmonary Medicine, 15 (99), 1-9. (doi:10.1186/s12890-015-0096-x). (PMID:26303256)

Record type: Article

Abstract

Background

Epidemiological evidence demonstrates that exposure to traffic-derived pollution worsens respiratory symptoms in asthmatics, but controlled human exposure studies have failed to provide a mechanism for this effect. Here we investigated whether diesel exhaust (DE) would induce apoptosis or proliferation in the bronchial epithelium in vivo and thus contribute to respiratory symptoms.

Methods

Moderate (n?=?16) and mild (n?=?16) asthmatics, atopic non-asthmatic controls (rhinitics) (n?=?13) and healthy controls (n?=?21) were exposed to filtered air or DE (100 ?g/m 3 ) for 2 h, on two separate occasions. Bronchial biopsies were taken 18 h post-exposure and immunohistochemically analysed for pro-apoptotic and anti-apoptotic proteins (Bad, Bak, p85 PARP, Fas, Bcl-2) and a marker of proliferation (Ki67). Positive staining was assessed within the epithelium using computerized image analysis.

Results

No evidence of epithelial apoptosis or proliferation was observed in healthy, allergic or asthmatic airways following DE challenge.

Conclusion

In the present study, we investigated whether DE exposure would affect markers of proliferation and apoptosis in the bronchial epithelium of asthmatics, rhinitics and healthy controls, providing a mechanistic basis for the reported increased airway sensitivity in asthmatics to air pollutants. In this first in vivo exposure investigation, we found no evidence of diesel exhaust-induced effects on these processes in the subject groups investigated.

Text
s12890-015-0096-x.pdf - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 11 August 2015
Published date: 25 August 2015
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 384294
URI: https://eprints.soton.ac.uk/id/eprint/384294
ISSN: 1471-2466
PURE UUID: 4b938667-b908-415c-9103-b9ef29061dfa

Catalogue record

Date deposited: 20 Nov 2015 14:46
Last modified: 17 Jul 2019 20:11

Export record

Altmetrics

Contributors

Author: Annelie F. Behndig
Author: Karthika Shanmuganathan
Author: Laura Whitmarsh
Author: Nikolai Stenfors
Author: Joanna L. Brown
Author: Anthony J. Frew
Author: Frank J. Kelly
Author: Ian S. Mudway
Author: Thomas Sandström
Author: Susan J. Wilson

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×