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Tapasin enhances MHC class I peptide presentation according to peptide half-life

Tapasin enhances MHC class I peptide presentation according to peptide half-life
Tapasin enhances MHC class I peptide presentation according to peptide half-life
Understanding how peptides are selected for presentation by MHC class I is crucial to vaccination strategies based on cytotoxic T lymphocyte priming. We have studied this selection of the MHC class I peptide repertoire in terms of the presentation of a series of individual peptides with a wide range of binding to MHC class I. This series was expressed as minigenes, and the presentation of each peptide variant was determined with the same MHC class I peptide-specific antibody. In wild-type cells, the hierarchy of presentation followed peptide half-life. This hierarchy broke down in cells lacking tapasin but not in cells lacking calreticulin or in cells lacking transporter associated with antigen processing-associated ERp57. We demonstrate a key role for tapasin in shaping the MHC class I peptide repertoire, as enhancement of presentation in the presence of tapasin correlated with peptide half-life.
0027-8424
11737-11742
Howarth, Mark
75054a4c-d313-4a69-ba86-8a08104ca300
Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Tolstrup, Anne B.
7b46dcbd-6410-41e5-aa23-097a0f7cdeb8
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Howarth, Mark
75054a4c-d313-4a69-ba86-8a08104ca300
Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Tolstrup, Anne B.
7b46dcbd-6410-41e5-aa23-097a0f7cdeb8
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e

Howarth, Mark, Williams, Anthony, Tolstrup, Anne B. and Elliott, Tim (2004) Tapasin enhances MHC class I peptide presentation according to peptide half-life Proceedings of the National Academy of Sciences, 101, (32), pp. 11737-11742. (doi:10.1073/pnas.0306294101). (PMID:15286279).

Record type: Article

Abstract

Understanding how peptides are selected for presentation by MHC class I is crucial to vaccination strategies based on cytotoxic T lymphocyte priming. We have studied this selection of the MHC class I peptide repertoire in terms of the presentation of a series of individual peptides with a wide range of binding to MHC class I. This series was expressed as minigenes, and the presentation of each peptide variant was determined with the same MHC class I peptide-specific antibody. In wild-type cells, the hierarchy of presentation followed peptide half-life. This hierarchy broke down in cells lacking tapasin but not in cells lacking calreticulin or in cells lacking transporter associated with antigen processing-associated ERp57. We demonstrate a key role for tapasin in shaping the MHC class I peptide repertoire, as enhancement of presentation in the presence of tapasin correlated with peptide half-life.

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Published date: 10 August 2004
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 384927
URI: http://eprints.soton.ac.uk/id/eprint/384927
ISSN: 0027-8424
PURE UUID: 64cd0033-ea32-4c42-8c85-05f8984b2988
ORCID for Tim Elliott: ORCID iD orcid.org/0000-0003-1097-0222

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Date deposited: 21 Jan 2016 11:02
Last modified: 17 Jul 2017 20:01

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Contributors

Author: Mark Howarth
Author: Anne B. Tolstrup
Author: Tim Elliott ORCID iD

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