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Tapasin enhances MHC class I peptide presentation according to peptide half-life

Record type: Article

Understanding how peptides are selected for presentation by MHC class I is crucial to vaccination strategies based on cytotoxic T lymphocyte priming. We have studied this selection of the MHC class I peptide repertoire in terms of the presentation of a series of individual peptides with a wide range of binding to MHC class I. This series was expressed as minigenes, and the presentation of each peptide variant was determined with the same MHC class I peptide-specific antibody. In wild-type cells, the hierarchy of presentation followed peptide half-life. This hierarchy broke down in cells lacking tapasin but not in cells lacking calreticulin or in cells lacking transporter associated with antigen processing-associated ERp57. We demonstrate a key role for tapasin in shaping the MHC class I peptide repertoire, as enhancement of presentation in the presence of tapasin correlated with peptide half-life.

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Howarth, Mark, Williams, Anthony, Tolstrup, Anne B. and Elliott, Tim (2004) Tapasin enhances MHC class I peptide presentation according to peptide half-life Proceedings of the National Academy of Sciences, 101, (32), pp. 11737-11742. (doi:10.1073/pnas.0306294101). (PMID:15286279).

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Published date: 10 August 2004
Organisations: Cancer Sciences


Local EPrints ID: 384927
ISSN: 0027-8424
PURE UUID: 64cd0033-ea32-4c42-8c85-05f8984b2988
ORCID for Tim Elliott: ORCID iD

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Date deposited: 21 Jan 2016 11:02
Last modified: 17 Jul 2017 20:01

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Author: Mark Howarth
Author: Anne B. Tolstrup
Author: Tim Elliott ORCID iD

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