Tapasin enhances MHC class I peptide presentation according to peptide half-life


Howarth, Mark, Williams, Anthony, Tolstrup, Anne B. and Elliott, Tim (2004) Tapasin enhances MHC class I peptide presentation according to peptide half-life Proceedings of the National Academy of Sciences of the United States of America, 101, (32), pp. 11737-11742. (doi:10.1073/pnas.0306294101). (PMID:15286279).

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Description/Abstract

Understanding how peptides are selected for presentation by MHC class I is crucial to vaccination strategies based on cytotoxic T lymphocyte priming. We have studied this selection of the MHC class I peptide repertoire in terms of the presentation of a series of individual peptides with a wide range of binding to MHC class I. This series was expressed as minigenes, and the presentation of each peptide variant was determined with the same MHC class I peptide-specific antibody. In wild-type cells, the hierarchy of presentation followed peptide half-life. This hierarchy broke down in cells lacking tapasin but not in cells lacking calreticulin or in cells lacking transporter associated with antigen processing-associated ERp57. We demonstrate a key role for tapasin in shaping the MHC class I peptide repertoire, as enhancement of presentation in the presence of tapasin correlated with peptide half-life.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1073/pnas.0306294101
ISSNs: 0027-8424 (print)
Organisations: Cancer Sciences
ePrint ID: 384927
Date :
Date Event
10 August 2004Published
Date Deposited: 21 Jan 2016 11:02
Last Modified: 17 Apr 2017 04:39
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/384927

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