Howarth, Mark, Williams, Anthony, Tolstrup, Anne B. and Elliott, Tim
Tapasin enhances MHC class I peptide presentation according to peptide half-life
Proceedings of the National Academy of Sciences of the United States of America, 101, (32), . (doi:10.1073/pnas.0306294101). (PMID:15286279).
PDF 2004 Tapasin enhances MHC class I peptide presentation according to peptide half life.pdf
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Understanding how peptides are selected for presentation by MHC class I is crucial to vaccination strategies based on cytotoxic T lymphocyte priming. We have studied this selection of the MHC class I peptide repertoire in terms of the presentation of a series of individual peptides with a wide range of binding to MHC class I. This series was expressed as minigenes, and the presentation of each peptide variant was determined with the same MHC class I peptide-specific antibody. In wild-type cells, the hierarchy of presentation followed peptide half-life. This hierarchy broke down in cells lacking tapasin but not in cells lacking calreticulin or in cells lacking transporter associated with antigen processing-associated ERp57. We demonstrate a key role for tapasin in shaping the MHC class I peptide repertoire, as enhancement of presentation in the presence of tapasin correlated with peptide half-life.
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